Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,658,893	G→A	*176* (TAG→TAA)	gadE →	gad regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,893	0	G	A	100.0%	220.7 / NA	68	*176* (TAG→TAA)	gadE	gad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/31);  total (37/31)

AGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAG  >  NC_000913/3658765‑3659024                                                                                                                                 |                                                                                                                                    aGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTACGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATa                                                                                                                           >  2:298306/1‑139 (MQ=255)           cGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGaa                                                                                                                 >  1:455962/1‑139 (MQ=255)               gACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTg                                                                                                             <  2:723594/139‑1 (MQ=255)                aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                            <  1:1098245/139‑1 (MQ=255)                aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                            >  1:1304972/1‑139 (MQ=255)                aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                            >  1:549144/1‑139 (MQ=255)                      aaTATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctg                                                                                                      >  1:857586/1‑139 (MQ=255)                                          aaaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGAt                                                                                  >  1:502429/1‑139 (MQ=255)                                           aaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                 <  2:502429/139‑1 (MQ=255)                                           aaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                 <  2:1220315/139‑1 (MQ=255)                                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCaa                                                                                                                      <  2:970292/97‑1 (MQ=255)                                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCaa                                                                                                                      >  1:970292/1‑97 (MQ=255)                                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                             >  2:1311652/1‑138 (MQ=255)                                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                             <  1:1311652/138‑1 (MQ=255)                                                   gAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                    >  1:1508001/1‑112 (MQ=255)                                                   gAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                    <  2:1508001/112‑1 (MQ=255)                                                             aTTATGTACAAGCTGAAACTAAGACGTATGAGCGTCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCg                                                               <  2:1195312/139‑1 (MQ=255)                                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa                                                           >  2:1243094/1‑139 (MQ=255)                                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa                                                           >  1:1117654/1‑139 (MQ=255)                                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACt                                                      >  2:821695/1‑139 (MQ=255)                                                                       aGCTGAGACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa                                                     >  1:811731/1‑139 (MQ=255)                                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                           >  2:968295/1‑96 (MQ=255)                                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                           <  1:968295/96‑1 (MQ=255)                                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                                <  2:1206698/139‑1 (MQ=255)                                                                                  aGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCt                                          >  1:471105/1‑139 (MQ=255)                                                                                      gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg                                                                                                 <  2:1130717/80‑1 (MQ=255)                                                                                      gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg                                                                                                 >  1:1130717/1‑80 (MQ=255)                                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                 >  1:1115511/1‑94 (MQ=255)                                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                 <  2:1115511/94‑1 (MQ=255)                                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGa                                   <  1:1243094/139‑1 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                                 <  2:1394774/103‑1 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                                 >  1:1394774/1‑103 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTaaa                                       >  1:1380815/1‑129 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTaaa                                       <  2:1380815/129‑1 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAt                             >  2:224691/1‑139 (MQ=255)                                                                                               aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAt                             >  2:1233706/1‑139 (MQ=255)                                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                             >  2:349033/1‑90 (MQ=255)                                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                             <  1:349033/90‑1 (MQ=255)                                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAtt                            <  2:455962/139‑1 (MQ=255)                                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTc                           >  2:635434/1‑139 (MQ=255)                                                                                                       aCCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTgg                     <  1:1233706/139‑1 (MQ=255)                                                                                                       aCCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTgg                     <  1:224691/139‑1 (MQ=255)                                                                                                         ccTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                   <  2:1304972/139‑1 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                              <  1:60026/124‑1 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                              >  2:60026/1‑124 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                         <  2:782279/129‑1 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                         <  1:1160782/129‑1 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                         >  2:1160782/1‑129 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                         >  1:782279/1‑129 (MQ=255)                                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAtgtg               >  2:1249541/1‑139 (MQ=255)                                                                                                               tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa                                                           >  1:207336/1‑93 (MQ=255)                                                                                                               tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa                                                           <  2:207336/93‑1 (MQ=255)                                                                                                               tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa             <  1:635434/139‑1 (MQ=255)                                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                   <  1:1049854/130‑1 (MQ=255)                                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                   >  2:1049854/1‑130 (MQ=255)                                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                    <  2:399844/48‑1 (MQ=255)                                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                    >  1:399844/1‑48 (MQ=255)                                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg                                                                                                 <  2:1368604/51‑1 (MQ=255)                                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg                                                                                                 >  1:1368604/1‑51 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATTCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCGTGGTATGTGTATCCACCAg     >  2:1004007/1‑139 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                          <  2:173205/86‑1 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                          >  1:1386236/1‑86 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                          >  1:173205/1‑86 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                          <  2:1386236/86‑1 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                              >  2:230767/1‑114 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                              <  1:230767/114‑1 (MQ=255)                                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg     >  1:189943/1‑139 (MQ=255)                                                                                                                          aTTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAg  >  2:403771/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                    AGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAG  >  NC_000913/3658765‑3659024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑