Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,768	G→A	*97* (TAG→TAA)	yiaG →	HTH_CROC1 family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,768	0	G	A	100.0%	173.7 / NA	54	*97* (TAG→TAA)	yiaG	HTH_CROC1 family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/28);  total (26/28)

GCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGA  >  NC_000913/3719634‑3719898                                                                                                                                       |                                                                                                                                   gCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACtt                                                                                                                                <  1:1318443/139‑1 (MQ=255)                         gCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                        <  2:974432/139‑1 (MQ=255)                         gCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                        <  2:1229802/139‑1 (MQ=255)                                        tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                           <  1:1283317/105‑1 (MQ=255)                                        tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                           >  2:1283317/1‑105 (MQ=255)                                         gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                          <  2:448285/121‑1 (MQ=255)                                         gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                          >  1:448285/1‑121 (MQ=255)                                         gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                        <  2:1252688/139‑1 (MQ=255)                                         gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                        <  1:1281689/139‑1 (MQ=255)                                         gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                        <  2:617965/139‑1 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTc                                                                                                     >  2:687814/1‑125 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTc                                                                                                     <  1:687814/125‑1 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                       >  2:748149/1‑139 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                       >  1:1252552/1‑139 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTATATCCACTTTa                                                                                                                    <  2:122194/110‑1 (MQ=255)                                          ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTATATCCACTTTa                                                                                                                    >  1:122194/1‑110 (MQ=255)                                           gAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCg                                                                                      <  1:1078896/139‑1 (MQ=255)                                                   aCGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCaa                                                                              >  2:426/1‑139 (MQ=255)                                                       gTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                          <  2:203208/139‑1 (MQ=255)                                                         gAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTGAAGCCAACCCGGCATTAAGGAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGaat                                                                        <  2:382610/139‑3 (MQ=255)                                                             ccTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                    >  1:976090/1‑139 (MQ=255)                                                                 cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                   >  2:730914/1‑104 (MQ=255)                                                                 cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                   <  1:730914/104‑1 (MQ=255)                                                                 cAAGTGCCGAACTAAAATTGATGCGTTTCATTCAAGCCAACCCGGCATTAAGTAAGCAGTTAATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATaa                                                                >  2:993190/1‑139 (MQ=255)                                                                          aaCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                                 <  1:774957/81‑1 (MQ=255)                                                                          aaCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                                 >  2:774957/1‑81 (MQ=255)                                                                                    gATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgttg                                             >  1:828053/1‑139 (MQ=255)                                                                                      tGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                           <  1:664269/139‑1 (MQ=255)                                                                                      tGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                           >  2:664269/1‑139 (MQ=255)                                                                                       gCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGaaa                                                        <  2:611241/125‑1 (MQ=255)                                                                                       gCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGaaa                                                        >  1:611241/1‑125 (MQ=255)                                                                                          tttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTCTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAc                                       <  2:828053/139‑1 (MQ=255)                                                                                            tGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                                 <  2:1182050/63‑1 (MQ=255)                                                                                            tGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                                 >  1:1182050/1‑63 (MQ=255)                                                                                                    gCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTa                                                                                                                    >  2:306061/1‑52 (MQ=255)                                                                                                    gCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTa                                                                                                                    <  1:306061/52‑1 (MQ=255)                                                                                                     ccAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCg                            <  1:211931/139‑1 (MQ=255)                                                                                                       aaCCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTg                          <  1:535587/139‑1 (MQ=255)                                                                                                        aCCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTgg                         >  2:767175/1‑139 (MQ=255)                                                                                                         cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGc                        >  1:689970/1‑139 (MQ=255)                                                                                                         cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGc                        >  2:232027/1‑139 (MQ=255)                                                                                                          ccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCt                       <  1:922663/139‑1 (MQ=255)                                                                                                                   agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgttg                                             <  1:347767/108‑1 (MQ=255)                                                                                                                   agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgttg                                             >  2:347767/1‑108 (MQ=255)                                                                                                                          aGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                         >  1:653295/1‑73 (MQ=255)                                                                                                                          aGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                         <  2:653295/73‑1 (MQ=255)                                                                                                                            ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                        >  2:368223/1‑56 (MQ=255)                                                                                                                            ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                        <  1:368223/56‑1 (MQ=255)                                                                                                                            ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt     >  2:1113156/1‑139 (MQ=255)                                                                                                                               aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                           >  2:1194785/1‑98 (MQ=255)                                                                                                                               aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                           <  1:1194785/98‑1 (MQ=255)                                                                                                                               aTGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGa  >  1:1331699/1‑139 (MQ=255)                                                                                                                                   aaTAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACg        >  1:1192819/1‑129 (MQ=255)                                                                                                                                   aaTAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACg        <  2:1192819/129‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGA  >  NC_000913/3719634‑3719898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑