Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,750,092	G→A	*287* (TAG→TAA)  E3K (GAG→AAG)	sgbU → sgbE →	putative L‑xylulose 5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,750,092	0	G	A	100.0%	158.4 / NA	49	*287* (TAG→TAA)  E3K (GAG→AAG)	sgbU sgbE	putative L‑xylulose 5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

ACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGT  >  NC_000913/3749971‑3750227                                                                                                                          |                                                                                                                                        aCTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGa                                                                                                                         <  2:845478/138‑1 (MQ=255) aCTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGa                                                                                                                         >  1:845478/1‑138 (MQ=255)     tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGc                                                                                                                    <  2:587089/139‑1 (MQ=255)      cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCt                                                                                                                   >  2:74284/1‑139 (MQ=255)      cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCt                                                                                                                   >  1:347645/1‑139 (MQ=255)          gTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTggcg                                                                                                               <  2:347645/139‑1 (MQ=255)                     aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGa                                                                                                                                <  2:536481/111‑1 (MQ=255)                     aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGa                                                                                                                                >  1:536481/1‑111 (MQ=255)                     aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgc                                                                                                    >  2:1256139/1‑139 (MQ=255)                                gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                              >  2:411903/1‑118 (MQ=255)                                gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                              <  1:411903/118‑1 (MQ=255)                                 aCCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        <  1:74284/139‑1 (MQ=255)                                                 agCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTc                                                                        <  2:189652/139‑1 (MQ=255)                                                            gagaTTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCaa                                                             <  2:1019939/139‑1 (MQ=255)                                                                ttattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTc                                                         >  1:486458/1‑139 (MQ=255)                                                                 tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCa                                                        >  2:774405/1‑139 (MQ=255)                                                                 tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCa                                                        >  1:308070/1‑139 (MQ=255)                                                                   ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTAAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGCTCACCTGGGGCAATGTCAg                                                       <  1:1208836/138‑1 (MQ=255)                                                                   ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTAAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGCTCACCTGGGGCAATGTCAg                                                       >  2:1208836/1‑138 (MQ=255)                                                                     cAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                    <  1:142853/139‑1 (MQ=255)                                                                        gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGaa                                                                                                                               >  2:1018841/1‑61 (MQ=255)                                                                        gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGaa                                                                                                                               <  1:1018841/61‑1 (MQ=255)                                                                         cgcgGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCt                                                                                                 >  1:394502/1‑90 (MQ=255)                                                                         cgcgGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCt                                                                                                 <  2:394502/90‑1 (MQ=255)                                                                         cgcgGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                              <  1:1444968/93‑1 (MQ=255)                                                                         cgcgGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                              >  2:1444968/1‑93 (MQ=255)                                                                             gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTg                                                                                                                  <  1:203157/69‑1 (MQ=255)                                                                             gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTg                                                                                                                  >  2:203157/1‑69 (MQ=255)                                                                                       gAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                  <  2:486458/139‑1 (MQ=255)                                                                                       gAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                  <  1:1256139/139‑1 (MQ=255)                                                                                             cgTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                            >  2:1375172/1‑139 (MQ=255)                                                                                             cgTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                            <  1:774405/139‑1 (MQ=255)                                                                                               tATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatgg                          >  1:1379283/1‑139 (MQ=255)                                                                                                     ggaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCa                    >  2:2122/1‑139 (MQ=255)                                                                                                       aggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGt                                                                               <  2:839523/78‑1 (MQ=255)                                                                                                       aggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGt                                                                               >  1:839523/1‑78 (MQ=255)                                                                                                        ggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                          <  2:1431596/82‑1 (MQ=255)                                                                                                        ggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                          >  1:1431596/1‑82 (MQ=255)                                                                                                        ggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                    <  2:664917/88‑1 (MQ=255)                                                                                                        ggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                    >  1:664917/1‑88 (MQ=255)                                                                                                         gCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                    >  2:598619/1‑87 (MQ=255)                                                                                                         gCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                    <  1:598619/87‑1 (MQ=255)                                                                                                          cTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                           >  1:860072/1‑127 (MQ=255)                                                                                                          cTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                           <  2:860072/127‑1 (MQ=255)                                                                                                              aTTTATATGTTAAAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                              >  2:1019740/1‑40 (MQ=255)                                                                                                              aTTTATATGTTAAAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                              <  1:1019740/40‑1 (MQ=255)                                                                                                                ttATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGc         >  2:743341/1‑139 (MQ=255)                                                                                                                    atGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCg     >  1:1099272/1‑139 (MQ=255)                                                                                                                       ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGt  >  2:209585/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                        ACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGT  >  NC_000913/3749971‑3750227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑