Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,789,352 G→A R244C (CGC→TGC)  waaH ← LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,789,3520GA100.0% 200.3 / NA 63R244C (CGC→TGC) waaHLPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/37);  total (26/37)

GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTA  >  NC_000913/3789217‑3789482
                                                                                                                                       |                                                                                                                                  
gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCATCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:129271/139‑1 (MQ=255)
gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:1078789/139‑1 (MQ=255)
gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:1035311/139‑1 (MQ=255)
gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGAGAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:1035787/139‑1 (MQ=255)
    gCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATc                                                                                                                             <  1:1232551/139‑1 (MQ=255)
           aGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:1396525/128‑1 (MQ=255)
           aGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  1:1396525/1‑128 (MQ=255)
              atatCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGt                                                                                                                   <  1:1167116/139‑1 (MQ=255)
              atatCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGt                                                                                                                   <  1:975044/139‑1 (MQ=255)
                atCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:1015905/123‑1 (MQ=255)
                atCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  2:1015905/1‑123 (MQ=255)
                  ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               <  2:758238/123‑1 (MQ=255)
                  ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               >  1:758238/1‑123 (MQ=255)
                  ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACg                                                                                                               >  1:1194017/1‑139 (MQ=255)
                  ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACg                                                                                                               >  1:896006/1‑139 (MQ=255)
                   cGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGcaggcagg                                                                                                                                  >  2:544446/1‑119 (MQ=255)
                   cGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGcaggcagg                                                                                                                                  <  1:544446/119‑1 (MQ=255)
                      ctctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               >  1:310076/1‑119 (MQ=255)
                      ctctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               <  2:310076/119‑1 (MQ=255)
                       tctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  1:41896/1‑116 (MQ=255)
                       tctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:41896/116‑1 (MQ=255)
                       tctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               <  1:1483371/118‑1 (MQ=255)
                       tctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                               >  2:1483371/1‑118 (MQ=255)
                         tttGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATaa                                                                                                        >  1:419695/1‑139 (MQ=255)
                                    cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGttttt                                                                                             >  2:53929/1‑139 (MQ=255)
                                    cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGttttt                                                                                             >  2:705942/1‑139 (MQ=255)
                                         cAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                        <  2:1194017/139‑1 (MQ=255)
                                            acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                        >  1:1367603/1‑136 (MQ=255)
                                            acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                        <  2:1367603/136‑1 (MQ=255)
                                                gCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCt                                                                                 <  2:419695/139‑1 (MQ=255)
                                                 cAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTa                                                                                >  1:670831/1‑139 (MQ=255)
                                                       cTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAAc                                                                          <  1:1370869/139‑1 (MQ=255)
                                                         cGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:1378447/82‑1 (MQ=255)
                                                         cGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  1:1378447/1‑82 (MQ=255)
                                                            aaGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                                     <  2:548183/139‑1 (MQ=255)
                                                              gTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa                                                                   <  1:508265/139‑1 (MQ=255)
                                                                tATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  1:644689/1‑75 (MQ=255)
                                                                tATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:644689/75‑1 (MQ=255)
                                                                 aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                                <  1:705942/139‑1 (MQ=255)
                                                                   ttGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTCATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                                     <  2:928749/132‑1 (MQ=255)
                                                                   ttGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                                     >  1:928749/1‑132 (MQ=255)
                                                                       tgatgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                        >  1:894762/1‑109 (MQ=255)
                                                                       tgatgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                        <  2:894762/109‑1 (MQ=255)
                                                                         atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAAc                                                                          <  2:1091615/121‑1 (MQ=255)
                                                                         atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAAc                                                                          >  1:1091615/1‑121 (MQ=255)
                                                                         atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                                <  2:1379515/131‑1 (MQ=255)
                                                                         atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                                >  1:1379515/1‑131 (MQ=255)
                                                                                   ggATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  2:1011749/56‑1 (MQ=255)
                                                                                   ggATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  1:1011749/1‑56 (MQ=255)
                                                                                     aTAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAatat                                            <  2:842612/139‑1 (MQ=255)
                                                                                         aTCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:574958/50‑1 (MQ=255)
                                                                                         aTCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  2:574958/1‑50 (MQ=255)
                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  2:78095/1‑45 (MQ=255)
                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:78095/45‑1 (MQ=255)
                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                                >  1:1474059/1‑110 (MQ=255)
                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                                <  2:1474059/110‑1 (MQ=255)
                                                                                                ttttGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGa                                 <  1:53929/139‑1 (MQ=255)
                                                                                                   tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                                                                                       <  1:747857/82‑1 (MQ=255)
                                                                                                   tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                                                                                       >  2:747857/1‑82 (MQ=255)
                                                                                                     tCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 <  1:330139/38‑1 (MQ=255)
                                                                                                     tCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                                 >  2:330139/1‑38 (MQ=255)
                                                                                                                       aaTTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                                     >  1:740761/1‑80 (MQ=255)
                                                                                                                       aaTTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                                     <  2:740761/80‑1 (MQ=255)
                                                                                                                               cagcagGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTa  <  2:435401/139‑1 (MQ=255)
                                                                                                                               cagcagGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTa  <  2:670831/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTA  >  NC_000913/3789217‑3789482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: