Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,584	G→A	*275* (TAG→TAA)	dinD →	DNA damage‑inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,584	0	G	A	100.0%	175.6 / NA	55	*275* (TAG→TAA)	dinD	DNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/27);  total (28/27)

TTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCATTT  >  NC_000913/3818457‑3818718                                                                                                                                |                                                                                                                                       ttGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaa                                                                                                                             >  1:1272872/1‑139 (MQ=255)   gACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaaa                                                                                                                           >  2:1490318/1‑139 (MQ=255)         ggTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCttt                                                                                                                     <  2:1272872/139‑1 (MQ=255)              cAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttgtt                                                                                                                >  1:720964/1‑139 (MQ=255)                            ccATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCg                                                                                                  <  1:1161417/139‑1 (MQ=255)                            ccATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCg                                                                                                  <  2:317003/139‑1 (MQ=255)                            ccATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCg                                                                                                  >  1:286536/1‑139 (MQ=255)                                       cTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGcc                                                                                       >  2:1282265/1‑139 (MQ=255)                                             ggAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTc                                                                                 <  1:1490318/139‑1 (MQ=255)                                                  cATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                            <  1:1282265/139‑1 (MQ=255)                                                     gCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                         >  2:1062325/1‑139 (MQ=255)                                                     gCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                         <  2:286536/139‑1 (MQ=255)                                                     gCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                         <  1:1062325/139‑1 (MQ=255)                                                          aggagTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGaa                                                                    <  2:598700/139‑1 (MQ=255)                                                              gTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGTAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                                <  2:553935/139‑1 (MQ=255)                                                               ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     >  1:1368644/1‑117 (MQ=255)                                                               ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     <  2:1368644/117‑1 (MQ=255)                                                               ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACaa                                                               >  2:1441952/1‑139 (MQ=255)                                                                tGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGc                                                                                        <  1:355156/113‑1 (MQ=255)                                                                tGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGc                                                                                        >  2:355156/1‑113 (MQ=255)                                                                tGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAgg                                                                                   >  2:236850/1‑118 (MQ=255)                                                                tGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAgg                                                                                   <  1:236850/118‑1 (MQ=255)                                                                 gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTATAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTcc                                                                                <  2:18837/120‑1 (MQ=255)                                                                 gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTcc                                                                                >  1:18837/1‑120 (MQ=255)                                                                    gACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCt                                                          >  2:1433920/1‑139 (MQ=255)                                                                     cccccGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            <  1:350493/103‑1 (MQ=255)                                                                     cccccGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            >  2:350493/2‑104 (MQ=255)                                                                      ccccGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAAc                                                                                                                          <  1:753090/73‑1 (MQ=255)                                                                      ccccGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAAc                                                                                                                          >  2:753090/1‑73 (MQ=255)                                                                         cGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAata                                                     >  2:214258/1‑139 (MQ=255)                                                                                 aGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGaa                                                                    >  1:619038/1‑116 (MQ=255)                                                                                 aGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGaa                                                                    <  2:619038/116‑1 (MQ=255)                                                                                   cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGaa                                                                                                                                   <  2:1211504/51‑1 (MQ=255)                                                                                   cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGaa                                                                                                                                   >  1:1211504/1‑51 (MQ=255)                                                                                   cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACcttt                                           >  1:168709/1‑139 (MQ=255)                                                                                        aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                                        >  1:1437420/1‑73 (MQ=255)                                                                                        aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                                        <  2:1437420/73‑1 (MQ=255)                                                                                        aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTACACCTTTCtttt                                      >  2:204908/1‑139 (MQ=255)                                                                                             ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            <  2:1201423/80‑1 (MQ=255)                                                                                             ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            >  1:1201423/1‑80 (MQ=255)                                                                                             ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     >  1:448811/1‑87 (MQ=255)                                                                                             ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     <  2:448811/87‑1 (MQ=255)                                                                                              tGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCt                                <  1:1441952/139‑1 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            >  2:957189/1‑70 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                            <  1:957189/70‑1 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                            <  2:155122/86‑1 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                            >  1:155122/1‑86 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTaa                       >  2:520574/1‑139 (MQ=255)                                                                                                       gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTaa                       <  1:520574/139‑1 (MQ=255)                                                                                                        tgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAAc                      <  1:204908/139‑1 (MQ=255)                                                                                                                ttACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTAttttt              >  1:813590/1‑139 (MQ=255)                                                                                                                  aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     >  2:155447/1‑66 (MQ=255)                                                                                                                  aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                                     <  1:155447/66‑1 (MQ=255)                                                                                                                  aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTc            >  2:1389036/1‑139 (MQ=255)                                                                                                                            aaTAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCAttt  <  1:214258/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                       TTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCATTT  >  NC_000913/3818457‑3818718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑