Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,842,455	C→T	*445* (TAG→TAA)	adeQ ←	adenine permease, high affinity, adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,842,455	0	C	T	100.0%	225.8 / NA	70	*445* (TAG→TAA)	adeQ	adenine permease, high affinity, adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

CCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTT  >  NC_000913/3842349‑3842582                                                                                                           |                                                                                                                                ccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACag                                                                                                 >  1:839311/1‑139 (MQ=255)           accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacc                                                                                       >  2:418855/1‑139 (MQ=255)                      tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                            <  1:988996/91‑1 (MQ=255)                      tACAGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                            >  2:988996/1‑91 (MQ=255)                        ccGCCAGCGTGGCGAGCATTGTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGACCTGCGACCACCACcacaca                                                                            >  2:901019/1‑137 (MQ=255)                        ccGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       <  2:250346/94‑1 (MQ=255)                        ccGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       >  1:250346/1‑94 (MQ=255)                        ccGCCAGCGTGGCGAGCATCATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  1:901019/137‑1 (MQ=255)                                             tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCGTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:149094/1‑139 (MQ=255)                                             tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  1:418855/139‑1 (MQ=255)                                                  aTCATTAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTTCGACCAGCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                                <  2:1384066/139‑1 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCTTCGCCAGTTATTAATccac                                                                                                                         <  2:1111275/66‑1 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCTTCGCCAGTTATTAATccac                                                                                                                         >  1:1111275/1‑66 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTg                                                                                            <  2:681899/95‑1 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTg                                                                                            >  1:681899/1‑95 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:350324/1‑111 (MQ=255)                                                  aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:350324/111‑1 (MQ=255)                                                            ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   <  1:1459454/94‑1 (MQ=255)                                                            ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   >  2:1459454/1‑94 (MQ=255)                                                            ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:877221/1‑124 (MQ=255)                                                            ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  2:877221/124‑1 (MQ=255)                                                            ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                      >  1:1151269/1‑139 (MQ=255)                                                                    tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                 <  2:1120969/136‑1 (MQ=255)                                                                    tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                 >  1:1120969/1‑136 (MQ=255)                                                                    tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAg                              >  2:143463/1‑139 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       >  1:314173/1‑47 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       <  2:314173/47‑1 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                      <  2:780172/80‑1 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                      >  1:780172/1‑80 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:889024/90‑1 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:889024/1‑90 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGGGCCAGCGCCCGGTGCCTACTTTCATGATGCAGTAc                           >  2:1156499/1‑139 (MQ=255)                                                                       tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc                           >  2:1318438/1‑139 (MQ=255)                                                                            aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACAt                      <  2:1151269/139‑1 (MQ=255)                                                                              tCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:618088/83‑1 (MQ=255)                                                                              tCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:618088/1‑83 (MQ=255)                                                                                aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                               <  1:1129506/94‑1 (MQ=255)                                                                                aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                               >  2:1129506/1‑94 (MQ=255)                                                                                 tGATCTCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcataca                                                                            <  2:987320/80‑1 (MQ=255)                                                                                 tGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:987320/1‑80 (MQ=255)                                                                                     atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAg             >  1:1125506/1‑139 (MQ=255)                                                                                     atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAg             >  2:423583/1‑139 (MQ=255)                                                                                        tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                           <  2:512277/106‑1 (MQ=255)                                                                                        tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                           >  1:512277/1‑106 (MQ=255)                                                                                        tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc          >  2:371335/1‑139 (MQ=255)                                                                                         ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa         <  2:149094/139‑1 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                           >  1:1077812/1‑72 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                           <  2:1077812/72‑1 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                      <  1:1095424/77‑1 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                      >  2:1095424/1‑77 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa        <  1:143463/139‑1 (MQ=255)                                                                                          gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa        <  2:1125506/139‑1 (MQ=255)                                                                                           aaaCCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAAt       >  1:1171937/1‑139 (MQ=255)                                                                                                cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCGAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTACAATCCCtt  >  2:907247/1‑139 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                        <  2:377480/51‑1 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                        >  1:377480/1‑51 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  2:1149165/1‑63 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:497207/1‑63 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:497207/63‑1 (MQ=255)                                                                                                  cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  1:1149165/63‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   <  1:507374/54‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   >  2:507374/1‑54 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  2:532142/84‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:532142/1‑84 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                              >  1:690881/1‑91 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                              <  2:690881/91‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                            >  1:761574/1‑109 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                            <  2:761574/109‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg               <  1:1192098/122‑1 (MQ=255)                                                                                                    ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg               >  2:1192098/1‑122 (MQ=255)                                                                                                           |                                                                                                                                CCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTT  >  NC_000913/3842349‑3842582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑