Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,892,455 G→A A281T (GCG→ACG)  mdtL → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,892,4550GA100.0% 140.0 / NA 44A281T (GCG→ACG) mdtLmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

GCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGC  >  NC_000913/3892324‑3892589
                                                                                                                                   |                                                                                                                                       
gcgGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGAt                                                                                                                                  <  1:219308/139‑1 (MQ=255)
                    aTGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCtt                                                                                                              <  1:1000806/139‑1 (MQ=255)
                          cTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATg                                                                                                        >  2:848924/1‑139 (MQ=255)
                                      gTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCATCTCGCAGGTGTTATTCCTCGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCTGTTTCTCTGt                                                                                           <  2:881855/139‑1 (MQ=255)
                                         aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:304791/1‑132 (MQ=255)
                                         aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:304791/132‑1 (MQ=255)
                                                     tCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                          <  2:916138/125‑1 (MQ=255)
                                                     tCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCGTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                          >  1:916138/1‑125 (MQ=255)
                                                             cACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                          <  1:195876/117‑1 (MQ=255)
                                                             cACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                          >  2:195876/1‑117 (MQ=255)
                                                             cACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                                    >  1:757945/1‑139 (MQ=255)
                                                                   aTTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGAt                                                                                                                                  <  2:749169/72‑1 (MQ=255)
                                                                   aTTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGAt                                                                                                                                  >  1:749169/1‑72 (MQ=255)
                                                                     ttGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGAt                                                                                                                                  >  1:15868/1‑70 (MQ=255)
                                                                     ttGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGAt                                                                                                                                  <  2:15868/70‑1 (MQ=255)
                                                                             ggAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCActct                                                                                                                            >  1:1472813/1‑68 (MQ=255)
                                                                             ggAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCActct                                                                                                                            <  2:1472813/68‑1 (MQ=255)
                                                                                    ttAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgtgt                                             <  2:1442416/139‑1 (MQ=255)
                                                                                          cACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGAt                                       <  2:516661/139‑1 (MQ=255)
                                                                                                        atCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCa                                                                                                          <  1:969010/59‑1 (MQ=255)
                                                                                                        atCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCa                                                                                                          >  2:969010/1‑59 (MQ=255)
                                                                                                          cACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:150158/1‑67 (MQ=255)
                                                                                                          cACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:150158/67‑1 (MQ=255)
                                                                                                          cACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGt                                                         <  2:1158542/106‑1 (MQ=255)
                                                                                                          cACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGt                                                         >  1:1158542/1‑106 (MQ=255)
                                                                                                           aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                                                                                               <  1:725054/67‑1 (MQ=255)
                                                                                                           aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                                                                                               >  2:725054/1‑67 (MQ=255)
                                                                                                           aCCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGc                      >  1:1340988/1‑139 (MQ=255)
                                                                                                               cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:730852/62‑1 (MQ=255)
                                                                                                               cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:72550/62‑1 (MQ=255)
                                                                                                               cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:730852/1‑62 (MQ=255)
                                                                                                               cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:72550/1‑62 (MQ=255)
                                                                                                               cGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtt                  >  1:393080/1‑139 (MQ=255)
                                                                                                                gCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGttt                 <  2:757945/139‑1 (MQ=255)
                                                                                                                 cAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtttt                >  2:246510/1‑139 (MQ=255)
                                                                                                                 cAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtttt                >  1:494929/1‑139 (MQ=255)
                                                                                                                     tgtTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGtt                                                                                                   >  1:1316876/1‑53 (MQ=255)
                                                                                                                     tgtTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGtt                                                                                                   <  2:1316876/53‑1 (MQ=255)
                                                                                                                       ttATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAc          >  2:718579/1‑139 (MQ=255)
                                                                                                                          ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg       <  1:246510/139‑1 (MQ=255)
                                                                                                                          ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg       <  1:718579/139‑1 (MQ=255)
                                                                                                                          ttCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg       <  1:848924/139‑1 (MQ=255)
                                                                                                                              tGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:804865/1‑47 (MQ=255)
                                                                                                                              tGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:804865/47‑1 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATg                                                                                                        <  2:783341/38‑1 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATg                                                                                                        >  1:783341/1‑38 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTAt                                                                                    <  1:407656/57‑1 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTAt                                                                                    >  2:407656/1‑57 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGc  >  2:408396/1‑139 (MQ=255)
                                                                                                                               ggcgacGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGc  >  2:34358/1‑139 (MQ=255)
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GCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGC  >  NC_000913/3892324‑3892589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: