Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,386	C→T	R78C (CGT→TGT)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,386	0	C	T	100.0%	114.4 / NA	37	R78C (CGT→TGT)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

GCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTA  >  NC_000913/3927254‑3927520                                                                                                                                     |                                                                                                                                       gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGa                                                                                                                                  >  1:1140951/1‑139 (MQ=255)        ttCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAgg                                                                                                                           >  2:797386/1‑139 (MQ=255)                  tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                                 >  2:123887/1‑139 (MQ=255)                        ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgc                                                                                                           <  2:1140951/139‑1 (MQ=255)                              cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGc                                                                                                     >  1:1358861/1‑139 (MQ=255)                                  cAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAg                                                                                                 <  1:797386/139‑1 (MQ=255)                                    ggATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGACGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAggg                                                                                               <  2:524205/139‑1 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGc                                                                                                                         >  1:449026/1‑102 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGc                                                                                                                         <  2:449026/102‑1 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                                          <  2:821899/117‑1 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                                          >  1:821899/1‑117 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAAGGCAACACGACTTCAGCGCGGGCGa                                                                                                   >  2:58952/1‑124 (MQ=255)                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAAGGCAACACGACTTCAGCGCGGGCGa                                                                                                   <  1:58952/124‑1 (MQ=255)                                                                               aaaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGCAGTGGCAATGTCAAACCTTAGGGCAACACATCTTCAGCGCGGGCGAAGGGATGTACACGCACATGAAAGCCCTTCGCCCCGATTAAGACCGTc                                                    >  2:598222/1‑139 (MQ=255)                                                                                aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCt                                                   >  2:251919/1‑139 (MQ=255)                                                                                      tGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTcc                                             >  1:400272/1‑139 (MQ=255)                                                                                            aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCa                                       >  1:1155544/1‑139 (MQ=255)                                                                                             tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                      <  2:709434/139‑1 (MQ=255)                                                                                                 cAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                  >  2:557820/1‑139 (MQ=255)                                                                                                 cAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCGTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                  <  2:717955/139‑1 (MQ=255)                                                                                                  aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAaca                                                                                                                     <  2:1003710/55‑1 (MQ=255)                                                                                                  aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAaca                                                                                                                     >  1:1003710/1‑55 (MQ=255)                                                                                                  aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                 <  1:557820/139‑1 (MQ=255)                                                                                                    ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              >  1:1011073/1‑92 (MQ=255)                                                                                                    ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCt                               >  2:646440/1‑139 (MQ=255)                                                                                                    ttCGAAGAGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              <  2:1011073/92‑1 (MQ=255)                                                                                                         aGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                                                                                                   >  1:695604/1‑66 (MQ=255)                                                                                                         aGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                                                                                                   <  2:695604/66‑1 (MQ=255)                                                                                                                cattcaCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              >  2:141427/1‑80 (MQ=255)                                                                                                                cattcaCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              <  1:141427/80‑1 (MQ=255)                                                                                                                     aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAc              <  2:1339898/139‑1 (MQ=255)                                                                                                                     aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAc              >  1:1339898/1‑139 (MQ=255)                                                                                                                       tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                           <  2:652857/108‑1 (MQ=255)                                                                                                                       tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                           >  1:652857/1‑108 (MQ=255)                                                                                                                                 gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATTAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                   <  2:991508/122‑1 (MQ=255)                                                                                                                                 gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa  >  1:1099981/1‑139 (MQ=255)                                                                                                                                 gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTGACCAGt                   >  1:991508/1‑122 (MQ=255)                                                                                                                                     |                                                                                                                                       GCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTA  >  NC_000913/3927254‑3927520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑