Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	349,847	T→C	intergenic (+275/‑165)	prpB → / → prpC	2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	349,847	0	T	C	100.0%	151.2 / NA	59	intergenic (+275/‑165)	prpB/prpC	2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

TCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAC  >  NC_000913/349724‑349981                                                                                                                            |                                                                                                                                       tCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATa                                                                                                                         >  1:1103251/1‑139 (MQ=17)    ggCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCg                                                                                                                      <  2:274636/139‑1 (MQ=17)       cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCg                                                                                                                   >  2:305620/1‑139 (MQ=17)              gTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGa                                                                                                            <  1:29177/139‑1 (MQ=17)               tGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                                           >  1:897343/1‑139 (MQ=17)               tGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                                           >  1:274636/1‑139 (MQ=18)                gCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGAt                                                                                                                                   <  1:1422936/114‑1 (MQ=18)                gCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGAt                                                                                                                                   >  2:1422936/1‑114 (MQ=18)                           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATa                                                                                                                                  <  1:1125676/104‑1 (MQ=18)                           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATa                                                                                                                                  >  2:1125676/1‑104 (MQ=17)                           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                               >  2:140062/1‑139 (MQ=37)                           tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                               >  1:244906/1‑139 (MQ=255)                                cGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcg                                                                                          >  1:1292124/1‑139 (MQ=37)                                cGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcg                                                                                          >  2:1104895/1‑139 (MQ=255)                                cGGATAAGACGAATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcg                                                                                          >  2:891173/1‑139 (MQ=25)                                 ggATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                                                         >  2:398080/1‑139 (MQ=255)                                   aTAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                                                       >  2:502768/1‑139 (MQ=255)                                       gACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                   <  1:305620/139‑1 (MQ=37)                                                cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGAt                                                                                                                                   >  2:423473/1‑82 (MQ=18)                                                cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGAt                                                                                                                                   <  1:423473/82‑1 (MQ=18)                                                cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCg                                                                                                                   >  2:1271117/1‑98 (MQ=21)                                                cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCg                                                                                                                   <  1:1271117/98‑1 (MQ=21)                                                 gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                                                         <  1:891173/139‑1 (MQ=17)                                                 gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                                                         <  1:398080/139‑1 (MQ=18)                                                   cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa                                                                       >  1:40237/1‑139 (MQ=18)                                                   cGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa                                                                       <  2:489558/139‑1 (MQ=17)                                                     cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                     >  1:148066/1‑139 (MQ=17)                                                      aTCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc                                                                    <  1:1351956/139‑1 (MQ=18)                                                             aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                                             <  2:40237/139‑1 (MQ=18)                                                             aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                                             <  2:1103251/139‑1 (MQ=18)                                                             aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                                             <  2:244906/139‑1 (MQ=17)                                                                  aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        <  1:502768/139‑1 (MQ=18)                                                                  aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        <  1:140062/139‑1 (MQ=18)                                                                    gCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaact                                                      <  1:398213/139‑1 (MQ=25)                                                                         aTGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                   >  1:1097164/1‑105 (MQ=18)                                                                         aTGCGCCGCTGACGCGACTTATCAGGCCCACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                   <  2:1097164/105‑1 (MQ=12)                                                                             gccgcTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAg                                             >  1:339215/1‑139 (MQ=21)                                                                              ccgcTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                   >  1:921321/1‑100 (MQ=21)                                                                              ccacTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCtta                                                                                   <  2:921321/97‑3 (MQ=255)                                                                                       cgACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAg                                   <  1:786777/139‑1 (MQ=18)                                                                                         aCTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        >  1:1475669/1‑116 (MQ=14)                                                                                         aCTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGGCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        <  2:1475669/116‑1 (MQ=9)                                                                                           ttATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        >  2:1153370/1‑114 (MQ=14)                                                                                           ttATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        <  1:1153370/114‑1 (MQ=14)                                                                                                 ggCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCg                         <  2:339215/139‑1 (MQ=17)                                                                                                  gCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt                        <  1:304011/139‑1 (MQ=18)                                                                                                    cTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg                      <  1:429820/139‑1 (MQ=17)                                                                                                    cTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg                      >  2:429820/1‑139 (MQ=17)                                                                                                       cGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCAt                   >  2:1373699/1‑139 (MQ=17)                                                                                                            tGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGAt                                      <  2:390322/115‑1 (MQ=17)                                                                                                            tGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGAt                                      >  1:390322/1‑115 (MQ=17)                                                                                                            tGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGac              >  2:304011/1‑139 (MQ=17)                                                                                                            tGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGac              >  1:3465/1‑139 (MQ=17)                                                                                                                     cTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc     <  1:1373699/139‑1 (MQ=38)                                                                                                                      tGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaact                                                      >  2:490099/1‑89 (MQ=25)                                                                                                                      tGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaact                                                      <  1:490099/89‑1 (MQ=25)                                                                                                                       gTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGa   <  2:1292124/139‑1 (MQ=255)                                                                                                                        tAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc  >  2:100018/1‑139 (MQ=255)                                                                                                                         aGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGa   <  1:13848/137‑1 (MQ=255)                                                                                                                            |                                                                                                                                       TCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAC  >  NC_000913/349724‑349981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑