Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,420	G→A	*33* (TAG→TAA)	ilvL →	ilvG operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,420	0	G	A	100.0%	247.9 / NA	76	*33* (TAG→TAA)	ilvL	ilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/36);  total (40/36)

TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554                                                                                                                                  |                                                                                                                                       ttCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGc                                                                                                                               >  1:334145/1‑139 (MQ=255)  tCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGcc                                                                                                                              >  1:472318/1‑139 (MQ=255)      tCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTaa                                                                                                                          <  1:1430304/139‑1 (MQ=255)        gAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACg                                                                                                                        >  2:1285900/1‑139 (MQ=255)              gATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  1:686308/1‑139 (MQ=255)               aTGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAg                                                                                                                 <  2:472318/139‑1 (MQ=255)                   aaGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAccc                                                                                                             >  2:11439/1‑139 (MQ=255)                               aaTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAg                                                                                                 >  1:779570/1‑139 (MQ=255)                                     aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGGTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCgg                                                                                           >  2:77184/1‑139 (MQ=255)                                     aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  1:1435033/1‑116 (MQ=255)                                     aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  2:1435033/116‑1 (MQ=255)                                                  gTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGa                                                                              >  2:603729/1‑139 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAAc                                                                                                                         >  2:855735/1‑93 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAAc                                                                                                                         <  1:855735/93‑1 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  2:584626/1‑100 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  1:584626/100‑1 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCt                                                                           >  1:1479425/1‑139 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCt                                                                           >  2:1318498/1‑139 (MQ=255)                                                             ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACa                                                                   >  1:751210/1‑139 (MQ=255)                                                             ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACa                                                                   <  1:11439/139‑1 (MQ=255)                                                                   gATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCg                                                             >  1:1301973/1‑139 (MQ=255)                                                                        gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCgaggag                                                        <  1:1318498/139‑1 (MQ=255)                                                                          gtggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  1:1285900/139‑1 (MQ=255)                                                                            ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  2:1330034/139‑1 (MQ=255)                                                                            ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:1023257/139‑1 (MQ=255)                                                                            ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:275983/139‑1 (MQ=255)                                                                            ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:46851/139‑1 (MQ=255)                                                                             gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTg                                                                               >  2:180671/1‑111 (MQ=255)                                                                             gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTg                                                                               <  1:180671/111‑1 (MQ=255)                                                                              tggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                  <  1:603729/139‑1 (MQ=255)                                                                                      attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa                                          <  2:1479425/139‑1 (MQ=255)                                                                                        tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATaa                                                                >  2:914700/1‑115 (MQ=255)                                                                                        tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATaa                                                                <  1:914700/115‑1 (MQ=255)                                                                                          tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg                                                                                            <  1:526116/85‑1 (MQ=255)                                                                                          tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg                                                                                            >  2:526116/1‑85 (MQ=255)                                                                                          tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                      >  2:85124/1‑139 (MQ=255)                                                                                             cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca                                   <  1:85124/139‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAAc                                                                                                                         <  1:845113/51‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAAc                                                                                                                         >  2:845113/1‑51 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  1:400041/1‑58 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  2:400041/58‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCg                                                                                                          >  1:625275/1‑66 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCg                                                                                                          <  2:625275/66‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACa                                                                   <  1:969861/105‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACa                                                                   >  2:969861/1‑105 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                 >  1:177399/1‑139 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                 >  1:1282969/1‑139 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                 >  2:390090/1‑139 (MQ=255)                                                                                                 gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                               >  2:309928/1‑139 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          <  2:695858/95‑1 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          >  1:695858/1‑95 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAtt                              >  1:857042/1‑139 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAtt                              >  2:1343509/1‑139 (MQ=255)                                                                                                    cGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca                                   <  1:1103800/132‑1 (MQ=255)                                                                                                    cGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca                                   >  2:1103800/1‑132 (MQ=255)                                                                                                    cGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                            >  1:964123/1‑139 (MQ=255)                                                                                                     ggggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                           >  2:698558/1‑139 (MQ=255)                                                                                                       ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                         >  2:298953/1‑139 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGt                                                                                       <  1:790622/72‑1 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGt                                                                                       >  2:790622/1‑72 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  2:454467/105‑1 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      >  1:454467/1‑105 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                    <  1:698558/139‑1 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCg                                                                                                          >  2:253202/1‑48 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCg                                                                                                          <  1:253202/48‑1 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt               >  2:75959/1‑139 (MQ=255)                                                                                                                   aCGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa             <  2:964123/139‑1 (MQ=255)                                                                                                                       ggAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc         <  2:751210/139‑1 (MQ=255)                                                                                                                        gAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg        >  1:60725/1‑139 (MQ=255)                                                                                                                        gAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg        <  1:714595/139‑1 (MQ=255)                                                                                                                             gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   <  2:869026/43‑1 (MQ=255)                                                                                                                             gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   >  1:869026/1‑43 (MQ=255)                                                                                                                             gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaa   <  1:309928/139‑1 (MQ=255)                                                                                                                             gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaa   <  2:177399/139‑1 (MQ=255)                                                                                                                             gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaa   <  1:1343509/139‑1 (MQ=255)                                                                                                                              cTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac  <  1:298953/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑