| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,078,438 | G→A | *330* (TAG→TAA) | yiiD → | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,078,438 | 0 | G | A | 98.2% | 176.6 / ‑4.3 | 57 | *330* (TAG→TAA) | yiiD | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (28/28); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
CGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGT > NC_000913/4078313‑4078558 | cGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAggg > 2:337137/1‑139 (MQ=255) tGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg > 1:1098800/1‑139 (MQ=255) cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg > 1:1041330/1‑139 (MQ=255) cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg > 1:390195/1‑139 (MQ=255) tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:1424921/139‑1 (MQ=255) tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:337137/139‑1 (MQ=255) tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:274077/139‑1 (MQ=255) ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctct > 2:899964/1‑139 (MQ=255) tCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGgag > 2:1076294/1‑119 (MQ=255) tCGAAATCTTGGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGgag < 1:1076294/119‑1 (MQ=255) tCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGA‑GGGCGGTGCTGCCTCTCTCATTCAg < 2:627705/139‑1 (MQ=255) tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg < 1:204123/139‑1 (MQ=255) gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCGCGCGAAGCCATTTCGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 2:1098800/139‑1 (MQ=255) gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 2:1041330/139‑1 (MQ=255) gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 1:149751/139‑1 (MQ=255) gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 1:899964/139‑1 (MQ=255) cgaGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTtat > 2:1439991/1‑139 (MQ=255) cgaGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTtat > 1:621522/1‑139 (MQ=255) ttttAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGTCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAtt < 2:1119288/137‑1 (MQ=255) tttGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGTCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAtt > 1:1119288/1‑137 (MQ=255) tGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg < 2:390195/139‑1 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGTCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt < 2:307272/107‑1 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt > 1:307272/1‑107 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt > 1:1193808/1‑106 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 2:1193808/106‑1 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat > 1:1362134/1‑137 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat < 2:1362134/137‑1 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTa > 1:1493260/1‑139 (MQ=255) tCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAg < 1:1439991/139‑1 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTtata < 1:1325639/125‑1 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTtata > 2:1325639/1‑125 (MQ=255) tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 1:419336/1‑76 (MQ=255) tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 2:419336/76‑1 (MQ=255) cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:51640/74‑1 (MQ=255) cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 2:51640/1‑74 (MQ=255) cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg < 1:1050743/117‑1 (MQ=255) cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg > 2:1050743/1‑117 (MQ=255) cgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc < 2:979659/86‑1 (MQ=255) cgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc > 1:979659/1‑86 (MQ=255) cATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCGGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTAt > 2:143983/1‑139 (MQ=255) aTTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAt < 2:1220206/101‑1 (MQ=255) aTTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAt > 1:1220206/1‑101 (MQ=255) gCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAg < 2:1197527/71‑1 (MQ=255) gCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAg > 1:1197527/1‑71 (MQ=255) ccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGtt < 2:1493260/139‑1 (MQ=255) tATGAAGAGGGCGGGAACGAAGCAGAGTAACTGATGGAGAGGGGCGGTGCTGCATCTCTCATTCAgg < 2:1083444/67‑1 (MQ=255) tATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg > 1:1496507/1‑44 (MQ=255) tATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg < 2:1496507/44‑1 (MQ=255) tATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg > 1:1083444/1‑67 (MQ=255) ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgt > 2:656871/1‑139 (MQ=255) gtgggAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctga < 1:942226/41‑2 (MQ=255) gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 2:942226/1‑43 (MQ=255) gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGCCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg > 2:956259/1‑139 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg < 1:445772/76‑1 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg > 2:445772/1‑76 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtgt > 2:189195/1‑139 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtgt < 2:621522/139‑1 (MQ=255) | CGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGT > NC_000913/4078313‑4078558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |