Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,148,681 C→T G529S (GGC→AGC)  eptC ← LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,148,6810CT100.0% 212.9 / NA 67G529S (GGC→AGC) eptCLPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/34);  total (33/34)

ATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTA  >  NC_000913/4148550‑4148807
                                                                                                                                   |                                                                                                                              
aTCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCaaa                                                                                                                         >  2:706842/1‑139 (MQ=255)
       tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTGACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  >  1:815103/1‑139 (MQ=255)
       tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  <  2:961454/139‑1 (MQ=255)
       tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  <  1:114211/139‑1 (MQ=255)
       tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  >  1:1481724/1‑139 (MQ=255)
            cAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgtg                                                                                                             <  2:1481724/139‑1 (MQ=255)
               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                          >  1:1398681/1‑139 (MQ=255)
                gtgtCGTAATCGATCAGTTCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                         <  1:706842/139‑1 (MQ=255)
                 tgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTTCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                        >  2:218579/1‑139 (MQ=255)
                    cGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                     >  2:319122/1‑139 (MQ=255)
                          cGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCGGATGTGGATCAGTTCCGc                                                                                               <  2:1266989/139‑1 (MQ=255)
                           gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAg                                                                                                                >  1:962121/1‑121 (MQ=255)
                           gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAg                                                                                                                <  2:962121/121‑1 (MQ=255)
                             tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                            >  1:371902/1‑139 (MQ=255)
                               aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                          <  1:218579/139‑1 (MQ=255)
                               aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                          <  2:815103/139‑1 (MQ=255)
                                                  ggTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAGGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATc                                                                       >  1:212867/1‑139 (MQ=255)
                                                  ggTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATc                                                                       >  2:1359976/1‑139 (MQ=255)
                                                   gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTT‑‑GATCAACa                                                                         >  1:868798/1‑134 (MQ=255)
                                                   gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTT‑‑GATCAACa                                                                         <  2:868798/134‑1 (MQ=255)
                                                               gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAGGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                          <  2:212867/139‑1 (MQ=255)
                                                                     aGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCa                                                                                             >  2:591276/1‑98 (MQ=255)
                                                                     aGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCa                                                                                             <  1:591276/98‑1 (MQ=255)
                                                                           tttGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTc                                              >  1:433292/1‑139 (MQ=255)
                                                                                 cTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  <  1:660027/65‑1 (MQ=255)
                                                                                 cTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  >  2:660027/1‑65 (MQ=255)
                                                                                           accacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  <  2:1372486/55‑1 (MQ=255)
                                                                                           accacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                  >  1:1372486/1‑55 (MQ=255)
                                                                                           accacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           >  1:1246426/1‑78 (MQ=255)
                                                                                           accacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           <  2:1246426/78‑1 (MQ=255)
                                                                                           accacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAACCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTAGCTTGCCATTTTTCTg                              <  2:919394/139‑1 (MQ=255)
                                                                                              acTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                        <  2:1266971/62‑1 (MQ=255)
                                                                                              acTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                        >  1:1266971/1‑62 (MQ=255)
                                                                                              acTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGAGCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACg                           >  2:1023822/1‑139 (MQ=255)
                                                                                               cTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCa                                                                                             <  1:287687/72‑1 (MQ=255)
                                                                                               cTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCa                                                                                             >  2:287687/1‑72 (MQ=255)
                                                                                                 gAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                        >  1:26656/1‑43 (MQ=255)
                                                                                                 gAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                        <  2:26656/43‑1 (MQ=255)
                                                                                                 gAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           >  2:1211116/1‑72 (MQ=255)
                                                                                                 gAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           <  1:1211116/72‑1 (MQ=255)
                                                                                                  aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCca                       >  1:1007383/1‑139 (MQ=255)
                                                                                                       gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagca                  >  1:1299518/1‑139 (MQ=255)
                                                                                                        ttGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagcag                 <  1:1023822/139‑1 (MQ=255)
                                                                                                        ttGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagcag                 <  1:319122/139‑1 (MQ=255)
                                                                                                        ttGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagcag                 <  2:433292/139‑1 (MQ=255)
                                                                                                           gATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGaa              <  2:1299518/139‑1 (MQ=255)
                                                                                                            aTCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTGTTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCcac                      >  2:386434/1‑130 (MQ=255)
                                                                                                            aTCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCcac                      <  1:386434/130‑1 (MQ=255)
                                                                                                               gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa          <  1:1359976/139‑1 (MQ=255)
                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                     >  1:934613/1‑47 (MQ=255)
                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                     <  2:934613/47‑1 (MQ=255)
                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           >  1:1140706/1‑57 (MQ=255)
                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                           <  2:1140706/57‑1 (MQ=255)
                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa         <  2:1007383/139‑1 (MQ=255)
                                                                                                                 aaCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgtg                                                                                                             <  1:714875/38‑1 (MQ=38)
                                                                                                                 aaCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgtg                                                                                                             >  2:714875/1‑38 (MQ=38)
                                                                                                                 aaCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGaa              <  2:220102/133‑1 (MQ=255)
                                                                                                                 aaCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGaa              >  1:220102/1‑133 (MQ=255)
                                                                                                                  aCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                          >  1:712933/1‑88 (MQ=255)
                                                                                                                  aCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                          <  2:712933/88‑1 (MQ=255)
                                                                                                                      tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCGGTTCCGCCAGGCTGTATTTa                                                                                  >  2:1225946/1‑60 (MQ=255)
                                                                                                                      tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCGGTTCCGCCAGGCTGTATTTa                                                                                  <  1:1225946/60‑1 (MQ=255)
                                                                                                                       cgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCt                                                                                         <  2:519109/52‑1 (MQ=255)
                                                                                                                       cgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCt                                                                                         >  1:519109/1‑52 (MQ=255)
                                                                                                                       cgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTa  >  1:1109002/1‑139 (MQ=255)
                                                                                                                             aTAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                            <  2:224443/43‑1 (MQ=255)
                                                                                                                             aTAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                            >  1:224443/1‑43 (MQ=255)
                                                                                                                                   |                                                                                                                              
ATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTA  >  NC_000913/4148550‑4148807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑