Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,645 G→A *442* (TAG→TAA
N429N (AAC→AAT
zraR →
purD ←
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,6450GA100.0% 212.1 / NA 66*442* (TAG→TAA
N429N (AAC→AAT
zraR
purD
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/32);  total (34/32)

TATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGC  >  NC_000913/4204513‑4204775
                                                                                                                                    |                                                                                                                                  
tatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGc                                                                                                                              <  2:804884/139‑1 (MQ=255)
        cGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTc                                                                                                                      >  2:85689/1‑139 (MQ=255)
                              ggTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCt                                                                                                >  2:234364/1‑139 (MQ=255)
                                     cTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGc                                                                                         <  1:85689/139‑1 (MQ=255)
                                         cggcgCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGaa                                                                                     <  2:1191009/139‑1 (MQ=255)
                                         cggcgCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCACAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGaa                                                                                     >  1:297941/1‑139 (MQ=255)
                                             gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                 <  1:37895/139‑1 (MQ=255)
                                              cTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                 <  2:964985/138‑1 (MQ=255)
                                              cTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                 >  1:964985/1‑138 (MQ=255)
                                                           gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGc                                                                                   <  2:720733/123‑1 (MQ=255)
                                                           gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGc                                                                                   >  1:720733/1‑123 (MQ=255)
                                                           gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           <  2:654070/131‑1 (MQ=255)
                                                           gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           >  1:654070/1‑131 (MQ=255)
                                                                aacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGa                                                                                      <  2:4415/115‑1 (MQ=255)
                                                                aacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGa                                                                                      >  1:4415/1‑115 (MQ=255)
                                                                         gAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAgg                                                     >  2:425551/1‑139 (MQ=255)
                                                                                cccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgc                                              >  2:1414629/1‑139 (MQ=255)
                                                                                 ccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGAt                                                                                                                   <  2:243916/69‑1 (MQ=255)
                                                                                 ccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGAt                                                                                                                   >  1:243916/1‑69 (MQ=255)
                                                                                       gTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           <  1:1429989/103‑1 (MQ=255)
                                                                                       gTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           >  2:1429989/1‑103 (MQ=255)
                                                                                          aGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTgc                                    >  2:645447/1‑139 (MQ=255)
                                                                                             gATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           >  2:1087840/1‑97 (MQ=255)
                                                                                             gATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           <  1:1087840/97‑1 (MQ=255)
                                                                                              aTCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            >  1:45373/1‑63 (MQ=255)
                                                                                              aTCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            <  2:45373/63‑1 (MQ=255)
                                                                                                cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                            >  2:365989/1‑93 (MQ=255)
                                                                                                cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                            <  1:365989/93‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                            <  1:295184/43‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                            >  2:295184/1‑43 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            <  1:1383314/59‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            >  2:1383314/1‑59 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCttt                                                                                              >  2:749637/1‑73 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCttt                                                                                              <  1:749637/73‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                <  2:567430/87‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                >  1:567430/1‑87 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                               <  1:171233/104‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                               >  2:171233/1‑104 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                             <  2:611373/106‑1 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                             >  1:611373/1‑106 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                            >  2:864566/1‑139 (MQ=255)
                                                                                                  cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                            >  2:652280/1‑139 (MQ=255)
                                                                                                        aaaCGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           <  2:994924/86‑1 (MQ=255)
                                                                                                        aaaCGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           >  1:994924/1‑86 (MQ=255)
                                                                                                             cTATTGGCAAAACTGTCGCGTTAATTCTGATCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAATCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAc                 >  1:1025995/1‑139 (MQ=255)
                                                                                                              tATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                <  2:63845/139‑1 (MQ=255)
                                                                                                                 tGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcg                                                                              >  1:1053897/1‑74 (MQ=255)
                                                                                                                 tGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcg                                                                              <  2:1053897/74‑1 (MQ=255)
                                                                                                                     aaaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGcgcg         <  1:645447/139‑1 (MQ=255)
                                                                                                                     aaaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGcgcg         <  1:1414629/139‑1 (MQ=255)
                                                                                                                      aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            <  1:1197914/39‑1 (MQ=255)
                                                                                                                      aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                            >  2:1197914/1‑39 (MQ=255)
                                                                                                                      aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCttt                                                                                              >  1:882944/1‑53 (MQ=255)
                                                                                                                      aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCttt                                                                                              <  2:882944/53‑1 (MQ=255)
                                                                                                                      aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCgg        <  1:864566/139‑1 (MQ=255)
                                                                                                                       aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                        <  2:1171759/42‑1 (MQ=255)
                                                                                                                       aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                        >  1:1171759/1‑42 (MQ=255)
                                                                                                                       aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           >  2:1473072/1‑71 (MQ=255)
                                                                                                                       aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                           <  1:1473072/71‑1 (MQ=255)
                                                                                                                            tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGa                                                                                      >  1:299580/1‑55 (MQ=255)
                                                                                                                            tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGa                                                                                      <  2:299580/55‑1 (MQ=255)
                                                                                                                            tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                               <  2:527590/78‑1 (MQ=255)
                                                                                                                            tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                               >  1:527590/1‑78 (MQ=255)
                                                                                                                            tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGc  >  1:1343011/1‑139 (MQ=255)
                                                                                                                             cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgt                                                                             >  1:84564/1‑63 (MQ=255)
                                                                                                                             cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgt                                                                             <  2:84564/63‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
TATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGC  >  NC_000913/4204513‑4204775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: