Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	217.6 / NA	60	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

AGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTC  >  NC_000913/4389798‑4390063                                                                                                                                         |                                                                                                                                  aGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCggtggtggt                                                                                                                                 >  2:429933/1‑139 (MQ=255)     aaaTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                             <  2:1054869/139‑1 (MQ=255)      aaTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcgc                                                                                                                            >  1:270444/1‑139 (MQ=255)                tcgttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                  <  2:1380988/139‑1 (MQ=255)                     aCGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTg                                                                                                             <  1:371538/139‑1 (MQ=255)                       gggCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                           <  1:657941/139‑1 (MQ=255)                                 aTTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                          >  2:1310382/1‑114 (MQ=255)                                 aTTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                          <  1:1310382/114‑1 (MQ=255)                                   tCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACg                                                                                                   >  1:868155/1‑135 (MQ=255)                                   tCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACg                                                                                                   <  2:868155/135‑1 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                           <  2:1243542/123‑1 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                           >  1:1243542/1‑123 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                           >  2:1308870/1‑139 (MQ=255)                                                  ccGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                <  1:1430221/139‑1 (MQ=255)                                                                 cGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                             >  1:47808/1‑79 (MQ=255)                                                                 cGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                             <  2:47808/79‑1 (MQ=255)                                                                    agaagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                              >  1:1169752/1‑123 (MQ=255)                                                                    agaagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                              <  2:1169752/123‑1 (MQ=255)                                                                       agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                >  2:823894/1‑118 (MQ=255)                                                                       agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                <  1:823894/118‑1 (MQ=255)                                                                           aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCggtggtggt                                                                                                                                 <  2:1347299/65‑1 (MQ=255)                                                                           aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                >  1:324874/1‑98 (MQ=255)                                                                           aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                <  2:324874/98‑1 (MQ=255)                                                                                ccGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGAACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGc                                                  >  1:1281016/1‑139 (MQ=255)                                                                                     aCGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACaa                                                                                                      >  2:1131857/1‑82 (MQ=255)                                                                                     aCGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACaa                                                                                                      <  1:1131857/82‑1 (MQ=255)                                                                                          gATCATCTCACGCAGAATACCGTTGCCCGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                        <  2:656772/139‑1 (MQ=255)                                                                                              atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                                <  1:890900/127‑1 (MQ=255)                                                                                              atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                        >  1:810998/1‑135 (MQ=255)                                                                                              atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTg                                        <  2:810998/135‑1 (MQ=255)                                                                                              atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGt                                    >  1:1244771/1‑139 (MQ=255)                                                                                              atcTAACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                                >  2:890900/1‑127 (MQ=255)                                                                                                 tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                              >  1:893167/1‑46 (MQ=255)                                                                                                 tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                              <  2:893167/46‑1 (MQ=255)                                                                                                   aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCAGAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTgg                               >  2:1047395/1‑139 (MQ=255)                                                                                                   aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGt                                                                                             >  1:700985/1‑77 (MQ=255)                                                                                                   aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGt                                                                                             <  2:700985/77‑1 (MQ=255)                                                                                                   aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGc                                          >  1:1280467/1‑128 (MQ=255)                                                                                                   aCGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGc                                          <  2:1280467/128‑1 (MQ=255)                                                                                                     gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGga                                                                                                                       >  2:1189581/1‑49 (MQ=255)                                                                                                     gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGga                                                                                                                       <  1:1189581/49‑1 (MQ=255)                                                                                                      cAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCt                            <  1:429933/139‑1 (MQ=255)                                                                                                         aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                >  1:1112522/1‑52 (MQ=255)                                                                                                         aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                <  2:1112522/52‑1 (MQ=255)                                                                                                         aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                         <  1:1308870/139‑1 (MQ=255)                                                                                                                ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa                  >  1:273118/1‑139 (MQ=255)                                                                                                                ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa                  >  1:451738/1‑139 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                  >  2:761105/1‑40 (MQ=39)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                  <  1:761105/40‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                >  2:458435/1‑42 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                <  1:458435/42‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                         <  1:882899/49‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                         >  2:882899/1‑49 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcctgc                                                           <  1:8600/95‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTtgcctgc                                                           >  2:8600/1‑95 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa               >  1:1370318/1‑139 (MQ=255)                                                                                                                        cATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGaa                                                                                      <  2:1392172/63‑1 (MQ=255)                                                                                                                        cATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAATGGTCACAAACGTACCGTTGCGGaa                                                                                      >  1:1392172/1‑63 (MQ=255)                                                                                                                             gtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                              <  2:136980/66‑1 (MQ=255)                                                                                                                             gtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                              >  1:136980/1‑66 (MQ=255)                                                                                                                               aCGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATcttctt   <  2:273118/139‑1 (MQ=255)                                                                                                                                cgcgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCttcttc  >  1:1237352/1‑139 (MQ=255)                                                                                                                                     tggtggTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCa                                                                           <  1:826627/61‑1 (MQ=255)                                                                                                                                     tggtggTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCa                                                                           >  2:826627/1‑61 (MQ=255)                                                                                                                                      ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                >  2:1065695/1‑39 (MQ=255)                                                                                                                                      ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                                <  1:1065695/39‑1 (MQ=255)                                                                                                                                      ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCa                                                                           >  1:351748/1‑60 (MQ=255)                                                                                                                                         |                                                                                                                                  AGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTC  >  NC_000913/4389798‑4390063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑