Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,422,793 C→T A203V (GCC→GTC)  ulaD → 3‑keto‑L‑gulonate 6‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,422,7930CT100.0% 164.9 / NA 51A203V (GCC→GTC) ulaD3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/23);  total (28/23)

GGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACG  >  NC_000913/4422672‑4422916
                                                                                                                         |                                                                                                                           
ggCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAAc                                                                                                            >  2:166181/1‑139 (MQ=255)
 gCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACg                                                                                                           >  1:1129549/1‑139 (MQ=255)
   ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGtt                                                                                                         >  1:583379/1‑139 (MQ=255)
         gtcaccgtcaccGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCg                                                                                                   <  2:583379/139‑1 (MQ=255)
          tcaccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGc                                                                                                  >  2:1125469/1‑139 (MQ=255)
                          ccTGGCGCTGGCAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              >  2:890035/1‑127 (MQ=255)
                          ccTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                      <  1:1331157/119‑1 (MQ=255)
                          ccTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                      >  2:1331157/1‑119 (MQ=255)
                          ccTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              <  1:890035/127‑1 (MQ=255)
                            tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                <  1:1125469/139‑1 (MQ=255)
                              gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                        <  2:204485/113‑1 (MQ=255)
                              gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                        >  1:204485/1‑113 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGtt                                                                                                                 <  2:32213/73‑1 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGtt                                                                                                                 >  1:32213/1‑73 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTggg                                                                                       <  2:1091320/99‑1 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTggg                                                                                       >  1:1091320/1‑99 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                   <  2:1510848/103‑1 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                   >  1:1510848/1‑103 (MQ=255)
                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg                                               >  1:849051/1‑139 (MQ=255)
                                                              tCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                              <  1:166181/139‑1 (MQ=255)
                                                                   ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                        <  1:1003402/76‑1 (MQ=255)
                                                                   ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                        >  2:1003402/1‑76 (MQ=255)
                                                                   ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCt                                                                                    >  2:433085/1‑96 (MQ=255)
                                                                   ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCt                                                                                    <  1:433085/96‑1 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              <  2:1400834/69‑1 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              >  1:1400834/1‑69 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTg                                                >  2:712087/1‑115 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTg                                                <  1:712087/115‑1 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATc                                           >  1:1261696/1‑120 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATc                                           <  2:1261696/120‑1 (MQ=255)
                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccg                        >  2:1515444/1‑139 (MQ=255)
                                                                                       cGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                           <  2:378115/69‑1 (MQ=255)
                                                                                       cGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                           >  1:378115/1‑69 (MQ=255)
                                                                                              tCCGTGATGCCGTTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTg              <  2:914608/139‑1 (MQ=255)
                                                                                                   gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                      >  2:1129189/1‑46 (MQ=255)
                                                                                                   gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                      <  1:1129189/46‑1 (MQ=255)
                                                                                                    aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAGACGTTCCATCACTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACGAATCCCGCTTg                                                >  1:976247/1‑99 (MQ=255)
                                                                                                    aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTg                                                <  2:976247/99‑1 (MQ=255)
                                                                                                    aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct        >  2:503588/1‑139 (MQ=255)
                                                                                                      gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                              <  2:1403282/99‑1 (MQ=255)
                                                                                                      gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                              >  1:1403282/1‑99 (MQ=255)
                                                                                                      gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg      >  1:114581/1‑139 (MQ=255)
                                                                                                       ccGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                             >  2:124284/1‑115 (MQ=255)
                                                                                                       ccGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                             <  1:124284/115‑1 (MQ=255)
                                                                                                          cTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                >  1:708456/1‑61 (MQ=255)
                                                                                                          cTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                <  2:708456/61‑1 (MQ=255)
                                                                                                          cTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACg  >  1:467553/1‑139 (MQ=255)
                                                                                                            tctcCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                           >  2:1488242/1‑48 (MQ=255)
                                                                                                            tctcCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                           <  1:1488242/48‑1 (MQ=255)
                                                                                                                  gTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              <  1:875962/39‑1 (MQ=255)
                                                                                                                  gTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                              >  2:875962/1‑39 (MQ=255)
                                                                                                                         |                                                                                                                           
GGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACG  >  NC_000913/4422672‑4422916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑