Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,386 G→A *229* (TAG→TAA)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,3860GA100.0% 136.5 / NA 43*229* (TAG→TAA) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/23);  total (20/23)

GATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAG  >  NC_000913/4424254‑4424519
                                                                                                                                    |                                                                                                                                     
gATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcg                                                                                                                                 <  1:829849/139‑1 (MQ=255)
  tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcgcg                                                                                                                               >  2:420720/1‑139 (MQ=255)
                 aaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                                <  1:61057/139‑1 (MQ=255)
                 aaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                                <  1:210821/139‑1 (MQ=255)
                     ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                            <  2:1418178/139‑1 (MQ=255)
                     ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                            <  1:420720/139‑1 (MQ=255)
                                                 caACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGc                                                                                >  1:295595/1‑139 (MQ=255)
                                                              tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                 >  2:1146631/1‑109 (MQ=255)
                                                              tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                 <  1:1146631/109‑1 (MQ=255)
                                                               cGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGAt                                                                                                    <  1:1499668/105‑1 (MQ=255)
                                                               cGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGAt                                                                                                    >  2:1499668/1‑105 (MQ=255)
                                                               cGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaa                                                                  >  1:812157/1‑139 (MQ=255)
                                                               cGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACAAGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCACCGTATATAACTGATCTAATTCCGCACCCGTTATTCaaaaaa                                                                  >  2:759086/1‑139 (MQ=255)
                                                                   aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACTCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCCGCCGTTTTTCAAAAAAAAGc                                                              <  1:759086/139‑1 (MQ=255)
                                                                   aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                              <  2:107526/139‑1 (MQ=255)
                                                                                         tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTc                                                                                                           >  2:580519/1‑72 (MQ=255)
                                                                                         tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTc                                                                                                           <  1:580519/72‑1 (MQ=255)
                                                                                         tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCg                                                                                    >  2:1023711/1‑95 (MQ=255)
                                                                                         tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCg                                                                                    <  1:1023711/95‑1 (MQ=255)
                                                                                          cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                                <  2:641406/66‑1 (MQ=255)
                                                                                          cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                                >  1:641406/1‑66 (MQ=255)
                                                                                                        gTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaaaa                                                                >  2:207964/1‑100 (MQ=255)
                                                                                                        gTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaaaa                                                                <  1:207964/100‑1 (MQ=255)
                                                                                                         tCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                        >  1:172570/1‑139 (MQ=255)
                                                                                                           cTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                      <  1:1435848/139‑1 (MQ=255)
                                                                                                             aaCGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTgg                                                                                              <  2:918576/65‑1 (MQ=255)
                                                                                                             aaCGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTgg                                                                                              >  1:918576/1‑65 (MQ=255)
                                                                                                             aaCGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                      >  1:336742/1‑137 (MQ=255)
                                                                                                             aaCGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                      <  2:336742/137‑1 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                           <  1:110047/62‑1 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                           >  2:110047/1‑62 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                           >  2:739796/1‑62 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                           <  1:739796/62‑1 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat              >  1:287850/1‑139 (MQ=255)
                                                                                                                   tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat              >  2:1342683/1‑139 (MQ=255)
                                                                                                                          ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                               <  2:343353/99‑1 (MQ=255)
                                                                                                                          ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                               >  1:343353/1‑99 (MQ=255)
                                                                                                                           gCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg      <  2:172570/139‑1 (MQ=255)
                                                                                                                               aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATgg                                >  2:21746/1‑109 (MQ=255)
                                                                                                                               aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATgg                                <  1:21746/109‑1 (MQ=255)
                                                                                                                               aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg  <  2:295595/139‑1 (MQ=255)
                                                                                                                                aGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                      <  1:798559/118‑1 (MQ=255)
                                                                                                                                aGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                      >  2:798559/1‑118 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAG  >  NC_000913/4424254‑4424519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑