Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,516 C→T *92* (TAG→TAA yjfY ← YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,5160CT100.0% 82.6 / NA 27*92* (TAG→TAAyjfYYhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

AAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGC  >  NC_000913/4424381‑4424650
                                                                                                                                       |                                                                                                                                      
aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     <  2:295595/139‑1 (MQ=255)
          cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGa                                                                                                                                  <  2:80167/132‑1 (MQ=255)
          cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGa                                                                                                                                  >  1:80167/1‑132 (MQ=255)
           gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATc                                                                                                                          >  2:1030949/1‑139 (MQ=255)
                                              gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     >  1:1422486/1‑93 (MQ=255)
                                              gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTc                                                                                      >  2:656842/1‑139 (MQ=255)
                                              gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTc                                                                                      >  2:1489028/1‑139 (MQ=255)
                                              gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATCGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     <  2:1422486/93‑1 (MQ=255)
                                               tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTcc                                                                                     <  1:1342683/139‑1 (MQ=255)
                                                    ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                <  1:1489028/139‑1 (MQ=255)
                                                    ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                <  1:656842/139‑1 (MQ=255)
                                                         cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                      <  1:895692/97‑1 (MQ=255)
                                                         cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                           >  1:1374789/1‑139 (MQ=255)
                                                         cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                           >  2:438605/1‑139 (MQ=255)
                                                         cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTCAGACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                      >  2:895692/1‑97 (MQ=255)
                                                             cGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAACCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAc                                                                       <  2:460574/139‑1 (MQ=255)
                                                               tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                      >  2:1075155/1‑91 (MQ=255)
                                                               tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                      <  1:1075155/91‑1 (MQ=255)
                                                                                            ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                <  2:1411660/68‑1 (MQ=255)
                                                                                            ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                >  1:1411660/1‑68 (MQ=255)
                                                                                                aagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttctt                                    <  2:287850/139‑1 (MQ=255)
                                                                                                      aCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                               <  2:1979/138‑1 (MQ=255)
                                                                                                      aCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                               >  1:1979/1‑138 (MQ=255)
                                                                                                           ggCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                         >  1:932802/1‑139 (MQ=255)
                                                                                                                            tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                      >  1:756482/1‑77 (MQ=255)
                                                                                                                            tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                      <  2:756482/77‑1 (MQ=255)
                                                                                                                            tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc        >  2:162626/1‑139 (MQ=255)
                                                                                                                                    gtgtTAGCGATAAATATCCGCGCTGGCGTAATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc  >  2:178291/1‑137 (MQ=255)
                                                                                                                                    gtgtTAGCGATAAATATCCGCGCTGGCGTAATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc  <  1:178291/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGC  >  NC_000913/4424381‑4424650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: