Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,391 G→A G90S (GGC→AGC)  bdcR → transcriptional repressor for divergent bdcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,3910GA96.8% 285.6 / ‑1.9 95G90S (GGC→AGC) bdcRtranscriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (45/47);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTC  >  NC_000913/4474255‑4474517
                                                                                                                                        |                                                                                                                              
ttATCTTGGTATTAACCCCGCGAGCCTCTACGCGGCTTTTGGTAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGc                                                                                                                              <  1:397467/139‑1 (MQ=255)
       ggTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGcc                                                                                                                       <  2:1324265/139‑1 (MQ=255)
       ggTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGcc                                                                                                                       >  1:1324265/1‑139 (MQ=255)
             aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         <  2:44507/131‑1 (MQ=255)
             aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         >  1:44507/1‑131 (MQ=255)
              aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                >  2:343142/1‑139 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGc                                                                                                                              <  2:473310/120‑1 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGc                                                                                                                              >  1:473310/1‑120 (MQ=255)
                       gCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTaaa                                                                                                       >  1:293776/1‑139 (MQ=255)
                          tctACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAaaga                                                                                                    >  1:961580/1‑139 (MQ=255)
                           ctACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAaagaa                                                                                                   <  1:1141435/139‑1 (MQ=255)
                             aCGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgc                                                                                                 >  1:739875/1‑139 (MQ=255)
                              cgcgGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcg                                                                                                <  1:1498454/139‑1 (MQ=255)
                              cgcgGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcg                                                                                                <  1:1345340/139‑1 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                <  2:520636/120‑1 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                >  1:520636/1‑120 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                >  1:1246752/1‑120 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGa                                                                                                                <  2:1246752/120‑1 (MQ=255)
                                   cTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                           >  1:1171388/1‑139 (MQ=255)
                                      ttGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcggcg                                                                                             <  2:244101/134‑1 (MQ=255)
                                      ttGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcggcg                                                                                             >  1:244101/1‑134 (MQ=255)
                                        ggCAGTAAAGCTGGGTTATTTAGCCGTGTGCTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAACCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGat                                                                                      >  2:919979/1‑139 (MQ=255)
                                        ggCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGat                                                                                      <  1:374840/139‑1 (MQ=255)
                                          cAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGatat                                                                                    <  2:739875/139‑1 (MQ=255)
                                             tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                        >  2:625304/1‑100 (MQ=255)
                                             tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGc                                                                                                                        <  1:625304/100‑1 (MQ=255)
                                             tAAAGCTGGGTTATTTAGCCGGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                >  1:385503/1‑139 (MQ=255)
                                                           ttAGCTGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACggcggc                                                                     >  2:203124/1‑137 (MQ=255)
                                                           ttAGCTGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACggcggc                                                                     <  1:203124/137‑1 (MQ=255)
                                                             aGCCGTTTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGTTATTAAAAGAGGCGGCGCGCAGTTATAGCCAAACGGCGGCTgcgc                                                                <  1:143264/139‑1 (MQ=255)
                                                             aGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcg                                                                 <  2:1171388/139‑1 (MQ=255)
                                                              gCCGTGTATTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAAGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                <  2:91923/139‑1 (MQ=255)
                                                               ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         >  2:1070999/1‑81 (MQ=255)
                                                               ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         <  1:1070999/81‑1 (MQ=255)
                                                               ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgct                                                               >  2:270944/1‑139 (MQ=255)
                                                                 gtgtACTCAATGAATACGTCGGTACGGAAGCTATTCAGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGc                                                                                                                              >  1:1290798/1‑74 (MQ=255)
                                                                  tgtACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctggc                                                            >  2:756082/1‑139 (MQ=255)
                                                                  tgtACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctggc                                                            <  2:1117391/139‑1 (MQ=255)
                                                                  tgtACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctggc                                                            >  1:1117391/1‑139 (MQ=255)
                                                                    tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctggctg                                                          >  1:120782/1‑139 (MQ=255)
                                                                      cTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                        <  2:385503/139‑1 (MQ=255)
                                                                      cTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                        <  1:756082/139‑1 (MQ=255)
                                                                       tCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTAt                                                       <  1:270944/139‑1 (MQ=255)
                                                                              aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCtt                                                >  1:219083/1‑139 (MQ=255)
                                                                                tataagagacagAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGa                                              >  2:786607/12‑139 (MQ=255)
                                                                                aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGtt                                                                                                                  >  1:1258600/1‑71 (MQ=255)
                                                                                aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGtt                                                                                                                  <  2:1258600/71‑1 (MQ=255)
                                                                                aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATg                                                      <  2:506242/131‑1 (MQ=255)
                                                                                aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATg                                                      >  1:506242/1‑131 (MQ=255)
                                                                                 cGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                             <  1:343142/139‑1 (MQ=255)
                                                                                 cGTAGGTACGGTAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                             <  1:993941/139‑1 (MQ=255)
                                                                                   tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAgg                                           >  2:1265608/1‑139 (MQ=255)
                                                                                   tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAgg                                           >  1:1314172/1‑139 (MQ=255)
                                                                                   tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAgg                                           >  1:1204427/1‑139 (MQ=255)
                                                                                     ggTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTa                                         >  1:356012/1‑139 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                <  1:383880/114‑1 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                >  2:383880/1‑114 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                               >  1:680571/1‑131 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                               <  2:680571/131‑1 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTAtt                                       >  2:1340512/1‑139 (MQ=255)
                                                                                         cGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                <  1:938924/112‑1 (MQ=255)
                                                                                         cGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                >  2:938924/1‑112 (MQ=255)
                                                                                             aGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGt                                 <  2:961580/139‑1 (MQ=255)
                                                                                                 aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                <  2:859753/104‑1 (MQ=255)
                                                                                                 aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                >  1:859753/1‑104 (MQ=255)
                                                                                                 aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATg                             <  2:219083/139‑1 (MQ=255)
                                                                                                       cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         >  2:989089/1‑41 (MQ=255)
                                                                                                       cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTg                                                                                                                         <  1:989089/41‑1 (MQ=255)
                                                                                                       cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                 >  2:218455/1‑49 (MQ=255)
                                                                                                       cTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTg                                                                                                                 <  1:218455/49‑1 (MQ=255)
                                                                                                            cGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcg                  <  2:293776/139‑1 (MQ=255)
                                                                                                                    ttcGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGatat                                                                                    <  1:940409/65‑1 (MQ=255)
                                                                                                                    ttcGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGatat                                                                                    >  2:940409/1‑65 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                               <  1:772898/36‑1 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAg                                                                                                               >  2:772898/1‑36 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                           >  2:186301/1‑56 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                           <  1:186301/56‑1 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                     >  1:857800/1‑110 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                     >  2:578993/1‑110 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                     <  1:578993/110‑1 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                     <  2:857800/110‑1 (MQ=255)
                                                                                                                         gatgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCt                                                 >  2:30367/1‑95 (MQ=255)
                                                                                                                         gatgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCt                                                 <  1:30367/95‑1 (MQ=255)
                                                                                                                         gatgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCg     <  1:1265608/139‑1 (MQ=255)
                                                                                                                         gatgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGACATTGCCg     <  2:120782/139‑1 (MQ=255)
                                                                                                                           tgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGtt   <  2:356012/139‑1 (MQ=255)
                                                                                                                           tgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGACGCGCAGATATAGCCAAAACGGCGGCTGCgctgg                                                             <  1:727862/81‑1 (MQ=255)
                                                                                                                           tgatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGACGCGCAGATATAGCCAAAACGGCGGCTGCgctgg                                                             >  2:727862/1‑81 (MQ=255)
                                                                                                                            gatCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTc  <  1:1340512/139‑1 (MQ=255)
                                                                                                                             atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                             >  1:264492/1‑95 (MQ=255)
                                                                                                                             atCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                             <  2:264492/95‑1 (MQ=255)
                                                                                                                               cGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCAt                              >  1:1148810/1‑108 (MQ=255)
                                                                                                                               cGTCCGGTAAGCGAGTGCCTGGTGGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCAt                              <  2:1148810/108‑1 (MQ=255)
                                                                                                                                     gTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGTGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                <  2:1249414/68‑1 (MQ=255)
                                                                                                                                     gTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGTGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                >  1:1249414/1‑68 (MQ=255)
                                                                                                                                        |                                                                                                                              
TTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTC  >  NC_000913/4474255‑4474517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: