Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 227.4 / NA 71*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/35);  total (36/35)

GTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGC  >  NC_000913/4490012‑4490275
                                                                                                                                 |                                                                                                                                      
cattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                               <  2:1011300‑M2/24‑1 (MQ=255)
    cccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  2:521962‑M2/112‑139 (MQ=255)
     ccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGcc                                                                                                                          <  2:366386‑M2/29‑1 (MQ=255)
                        tgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCg                                                                                                       <  1:521962‑M2/48‑1 (MQ=255)
                          tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcatcgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     >  2:630161‑M2/90‑139 (MQ=255)
                               ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGACAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  2:1240925‑M2/55‑1 (MQ=255)
                                  gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtatagccaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                             <  2:1097880‑M2/58‑1 (MQ=255)
                                      gggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcatcgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                         <  1:630161‑M2/62‑1 (MQ=255)
                                                      ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagcGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGCGATGTGGATGTGATCGTTa                                                                         >  2:1518727‑M2/62‑139 (MQ=255)
                                                        cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGtt                                                                          <  2:571152‑M2/77‑1 (MQ=255)
                                                        cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGtt                                                                          >  1:571152‑M2/60‑136 (MQ=255)
                                                           tcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAttttt                                                                    >  1:745904‑M2/57‑139 (MQ=255)
                                                                agatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAAttt                                                               >  2:1208111‑M2/52‑139 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     <  2:1492798‑M2/50‑1 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     >  1:1492798‑M2/47‑96 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                          >  2:1486484‑M2/47‑139 (MQ=255)
                                                                       ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  1:382989‑M2/45‑72 (MQ=255)
                                                                       ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           <  2:382989‑M2/28‑1 (MQ=255)
                                                                       ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtttt                                                                     <  1:43616‑M2/82‑1 (MQ=255)
                                                                       ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtttt                                                                     >  2:43616‑M2/45‑126 (MQ=255)
                                                                       ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg                                                        <  1:1208111‑M2/95‑1 (MQ=255)
                                                                         ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                      <  2:1470854‑M2/49‑1 (MQ=255)
                                                                         ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                      >  1:1470854‑M2/43‑91 (MQ=255)
                                                                         ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCa                                                      >  1:467580‑M2/43‑139 (MQ=255)
                                                                           agtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:1361278‑M2/55‑1 (MQ=255)
                                                                           agtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                >  2:1361278‑M2/41‑95 (MQ=255)
                                                                             tttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                                          >  2:909778‑M2/39‑83 (MQ=255)
                                                                             tttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                                          <  1:909778‑M2/45‑1 (MQ=255)
                                                                               tcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                >  2:720009‑M2/37‑91 (MQ=255)
                                                                               tcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:720009‑M2/55‑1 (MQ=255)
                                                                                cctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                    >  1:973555‑M2/36‑54 (MQ=255)
                                                                                cctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                    <  2:973555‑M2/19‑1 (MQ=255)
                                                                                    cctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                                           <  1:1486484‑M2/108‑1 (MQ=255)
                                                                                       ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           <  1:1203939‑M2/28‑1 (MQ=255)
                                                                                       ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  2:1203939‑M2/29‑56 (MQ=255)
                                                                                         agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     >  2:491470‑M2/27‑76 (MQ=255)
                                                                                         agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     <  1:491470‑M2/50‑1 (MQ=255)
                                                                                             taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  2:403262‑M2/23‑50 (MQ=255)
                                                                                             taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           <  1:403262‑M2/28‑1 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                >  2:949759‑M2/22‑76 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTACCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:949759‑M2/55‑1 (MQ=255)
                                                                                                  aacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAat                             >  1:986818‑M2/18‑139 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTg                                                             <  1:1333313‑M2/90‑1 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTg                                                             >  2:1333313‑M2/17‑106 (MQ=255)
                                                                                                    cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                        <  1:309672‑M2/47‑1 (MQ=255)
                                                                                                    cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                        >  2:309672‑M2/16‑62 (MQ=255)
                                                                                                    cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAatat                           >  2:1497813‑M2/16‑139 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGcc                                                                                                                          >  2:1490552‑M2/15‑43 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGcc                                                                                                                          <  1:1490552‑M2/29‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           <  2:560873‑M2/28‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  1:560873‑M2/15‑42 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                                    >  1:1482431‑M2/15‑49 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                                    <  2:1482431‑M2/35‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                        >  2:1507591‑M2/15‑61 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                        <  1:1507591‑M2/47‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                                            >  1:517127‑M2/15‑89 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                                            <  2:517127‑M2/75‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATc                          >  2:924170‑M2/15‑139 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATc                          >  2:528158‑M2/15‑139 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGGGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATc                          >  1:1341428‑M2/15‑139 (MQ=255)
                                                                                                      tccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                             >  1:1534142‑M2/14‑71 (MQ=255)
                                                                                                      tccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                             <  2:1534142‑M2/58‑1 (MQ=255)
                                                                                                           caaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACg                    <  2:986818‑M2/131‑1 (MQ=255)
                                                                                                               gagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGa                <  1:528158‑M2/135‑1 (MQ=255)
                                                                                                                agaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  2:1039589‑M2/55‑1 (MQ=255)
                                                                                                                agaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                >  1:1039589‑M2/4‑58 (MQ=255)
                                                                                                                       agaAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                                      <  2:1141513/109‑1 (MQ=255)
                                                                                                                       agaAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                                      >  1:1141513/1‑109 (MQ=255)
                                                                                                                            aTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTg                                               >  2:834983/1‑95 (MQ=255)
                                                                                                                            aTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTg                                               <  1:834983/95‑1 (MQ=255)
                                                                                                                             tAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGc  <  2:1341428/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                      
GTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGC  >  NC_000913/4490012‑4490275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: