Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,375	C→T	A255A (GCG→GCA)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,375	0	C	T	100.0%	160.9 / NA	50	A255A (GCG→GCA)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

CCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCG  >  NC_000913/4490240‑4490485                                                                                                                                        |                                                                                                               ccGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                             <  2:1489011/139‑1 (MQ=255)   gACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                           <  1:474974/139‑1 (MQ=255)          atCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCa                                                                                                    <  2:72697/139‑1 (MQ=255)              aaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAatat                                                                                                >  1:801657/1‑139 (MQ=255)                    ggAGTAATTACGCTGGCGAGGCTTGGGCTCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                          >  2:915255/1‑139 (MQ=255)                    ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgtgc                                                                                                              <  2:223893/119‑1 (MQ=255)                     gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                          >  1:1151186/1‑138 (MQ=255)                     gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                          <  2:1151186/138‑1 (MQ=255)                      aGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTg                                                                                        >  2:1504661/1‑139 (MQ=255)                                cTGGCGAGGCTTGGGATCATTTGCCAGCCAATCTCCAGCCAATAAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATa                                                                              <  1:844402/139‑1 (MQ=255)                                  ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                             <  2:581519/106‑1 (MQ=255)                                  ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                             >  1:581519/1‑106 (MQ=255)                                  ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                            <  2:261457/139‑1 (MQ=255)                                  ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                            <  2:186781/139‑1 (MQ=255)                                             ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATc                                                                                               >  1:937239/1‑109 (MQ=255)                                             ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATc                                                                                               <  2:937239/109‑1 (MQ=255)                                             ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCt                                                                      <  1:969588/134‑1 (MQ=255)                                             ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCt                                                                      >  2:969588/1‑134 (MQ=255)                                                  aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                                            <  1:1504661/139‑1 (MQ=255)                                                         tgccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                      <  1:580410/90‑1 (MQ=255)                                                         tgccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                      >  2:580410/1‑90 (MQ=255)                                                           ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCa                                                                                                         <  2:1118597/85‑1 (MQ=255)                                                           ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCa                                                                                                         >  1:1118597/1‑85 (MQ=255)                                                            cAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAg                                                  >  2:39664/1‑139 (MQ=255)                                                                 tcCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                             <  1:915255/139‑1 (MQ=255)                                                                     gCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                 <  1:12337/115‑1 (MQ=255)                                                                     gCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                 >  2:12337/1‑115 (MQ=255)                                                                      cccATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                             >  1:410978/1‑134 (MQ=255)                                                                      cccATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                             <  2:410978/134‑1 (MQ=255)                                                                         aTGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAtt                                     <  1:39664/139‑1 (MQ=255)                                                                           gAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                            >  2:99377/1‑98 (MQ=255)                                                                           gAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                            <  1:99377/98‑1 (MQ=255)                                                                              aaCCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTg                                <  1:1341039/139‑1 (MQ=255)                                                                                  cGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                           >  2:1396947/1‑60 (MQ=255)                                                                                  cGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                           <  1:1396947/60‑1 (MQ=255)                                                                                    cAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgc                                                        >  2:282592/1‑109 (MQ=255)                                                                                    cAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgc                                                        <  1:282592/109‑1 (MQ=255)                                                                                    cAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                             <  1:1431399/120‑1 (MQ=255)                                                                                    cAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                                             >  2:1431399/1‑120 (MQ=255)                                                                                     aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCt                                                     <  1:1114318/111‑1 (MQ=255)                                                                                     aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCt                                                     >  2:1114318/1‑111 (MQ=255)                                                                                      aTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGc                                        >  1:393816/1‑123 (MQ=255)                                                                                      aTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGc                                        <  2:393816/123‑1 (MQ=255)                                                                                             tttGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAat                                                                                                  >  1:1448132/1‑58 (MQ=255)                                                                                             tttGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAat                                                                                                  <  2:1448132/58‑1 (MQ=255)                                                                                                     ggCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                             <  2:839488/39‑1 (MQ=255)                                                                                                     ggCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCa                                                                                                             >  1:839488/1‑39 (MQ=255)                                                                                                         cGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAACTGAATGGATGAGATGgc     >  1:550180/1‑139 (MQ=255)                                                                                                           gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAat                                                                                                  >  2:560302/1‑44 (MQ=255)                                                                                                           gCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAat                                                                                                  <  1:560302/44‑1 (MQ=255)                                                                                                            cGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGcgcg  <  1:1497813/139‑1 (MQ=255)                                                                                                                                        |                                                                                                               CCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCG  >  NC_000913/4490240‑4490485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑