Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,500	G→A	*302* (TAG→TAA)	insD1 →	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,500	0	G	A	100.0%	265.0 / NA	86	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/47);  total (39/47)

GCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGT  >  NC_000913/4499375‑4499617                                                                                                                              |                                                                                                                      gCAGAGTCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  2:239791/139‑1 (MQ=11) gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  2:189705/139‑1 (MQ=11) gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  1:165820/139‑1 (MQ=11)          ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGa                                                                                                 >  1:866405/1‑139 (MQ=255)               gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGa                                                                                            >  2:856928/1‑139 (MQ=255)                cATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGaa                                                                                           <  2:866405/139‑1 (MQ=255)                     taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  2:63315/139‑1 (MQ=255)                     taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  1:856928/139‑1 (MQ=255)                     taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  1:1369718/139‑1 (MQ=255)                     taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  1:1203087/139‑1 (MQ=255)                       aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGat                                                                                    <  1:1187757/139‑1 (MQ=255)                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  1:730718/114‑1 (MQ=11)                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          >  2:730718/1‑114 (MQ=11)                           aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                >  1:767380/1‑139 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  1:653713/131‑1 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      >  1:1380611/1‑131 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      >  2:653713/1‑131 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  2:1380611/131‑1 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTta                                                                              >  2:732110/1‑139 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAAGTCTGGAAATATAAGGGCAAATACACCTTGTGCTGATATGAATACGGATATTTta                                                                              >  2:1378187/1‑139 (MQ=255)                               cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtata                                                                            <  1:1260835/139‑1 (MQ=255)                               cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtata                                                                            <  2:381483/139‑1 (MQ=255)                                   cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGa                                                                        <  2:1339093/139‑1 (MQ=255)                                   cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGa                                                                        >  1:1339093/1‑139 (MQ=255)                                        aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  2:687426/100‑1 (MQ=14)                                        aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          >  1:687426/1‑100 (MQ=14)                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          >  2:1380482/1‑98 (MQ=14)                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAcc                                                                                                          <  1:1380482/98‑1 (MQ=14)                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                  <  2:781022/138‑1 (MQ=255)                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                  >  1:781022/1‑138 (MQ=255)                                           gcgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                <  1:1378187/139‑1 (MQ=255)                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGat                                                                                    >  2:817556/1‑115 (MQ=255)                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGat                                                                                    <  1:817556/115‑1 (MQ=255)                                                gggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      <  2:1366335/112‑1 (MQ=255)                                                gggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                      >  1:1366335/1‑112 (MQ=255)                                                 ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTAc                                                          >  1:728129/1‑139 (MQ=255)                                                 ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTAc                                                          >  1:768030/1‑139 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          >  2:16731/1‑120 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          <  1:16731/120‑1 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAg                                                       >  2:1001918/1‑139 (MQ=255)                                                     aTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                      <  2:209537/139‑1 (MQ=255)                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGata                                                                                               <  1:432032/97‑1 (MQ=255)                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGata                                                                                               >  2:432032/1‑97 (MQ=255)                                                       cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                    <  2:768030/139‑1 (MQ=255)                                                         ctgtccacttgAATATATTCGGCAGCGGGCTTGTAATGGGTTATGTGAGAACAGATGTCTGGAAATATAAGTGGATATCGACATTGTGCTGATATGATTACGGATATTTTATACAGAATACTGTTCAGTACAAGCGACt                                                  <  2:1171924/129‑1 (MQ=21)                                                           cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                              >  1:423885/1‑125 (MQ=255)                                                           cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                              <  2:423885/125‑1 (MQ=255)                                                            gcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCGACCTtgtg                                                                                                     >  2:1100343/1‑85 (MQ=255)                                                            gcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTtgtg                                                                                                     <  1:1100343/85‑1 (MQ=255)                                                              cACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTAtt                                             >  2:1231388/1‑139 (MQ=255)                                                              cACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACAGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTAtt                                             <  1:1001918/139‑1 (MQ=255)                                                               aCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTa                                            <  1:732110/139‑1 (MQ=255)                                                                      tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                     <  1:648923/139‑1 (MQ=255)                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          <  1:829558/100‑1 (MQ=255)                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          >  2:829558/1‑100 (MQ=255)                                                                         cTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                  <  2:728129/139‑1 (MQ=255)                                                                          tGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCat                                 <  1:1231388/139‑1 (MQ=255)                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                        <  2:300496/129‑1 (MQ=255)                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                        >  1:300496/1‑129 (MQ=255)                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatata                              >  1:490589/1‑139 (MQ=255)                                                                              gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                      >  2:748099/1‑114 (MQ=255)                                                                              gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                      <  1:748099/114‑1 (MQ=255)                                                                                 gCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTtgtg                                                                                                     <  1:725079/64‑1 (MQ=255)                                                                                 gCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTtgtg                                                                                                     >  2:725079/1‑64 (MQ=255)                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          >  2:523351/1‑90 (MQ=255)                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          <  1:523351/90‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                      <  2:1158217/97‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                      >  1:1158217/1‑97 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                    <  2:1390384/99‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                    >  1:1390384/1‑99 (MQ=255)                                                                                                 ttAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa          >  1:1136984/1‑139 (MQ=255)                                                                                                 ttAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa          <  2:490589/139‑1 (MQ=255)                                                                                                    aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGa                           >  1:319752/1‑119 (MQ=255)                                                                                                    aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGa                           <  2:319752/119‑1 (MQ=255)                                                                                                    aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCagaaagaa       >  2:863478/1‑139 (MQ=255)                                                                                                         tAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                >  1:399823/1‑77 (MQ=255)                                                                                                         tAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                <  2:399823/77‑1 (MQ=255)                                                                                                         tAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATATTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGt  >  2:743429/1‑139 (MQ=255)                                                                                                               aTGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGat                                                                                                >  1:1306924/1‑39 (MQ=21)                                                                                                               aTGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGat                                                                                                <  2:1306924/39‑1 (MQ=21)                                                                                                               aTGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGa                 >  2:728554/1‑118 (MQ=255)                                                                                                               aTGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGa                 <  1:728554/118‑1 (MQ=255)                                                                                                                  tCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtg                    >  1:1431866/1‑112 (MQ=255)                                                                                                                  tCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtg                    <  2:1431866/112‑1 (MQ=255)                                                                                                                           ataAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          <  1:1307697/49‑1 (MQ=255)                                                                                                                           ataAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                          >  2:1307697/1‑49 (MQ=255)                                                                                                                              |                                                                                                                      GCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGT  >  NC_000913/4499375‑4499617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑