Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,612,289 G→A *54* (TAG→TAA ytjA → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,612,2890GA100.0% 86.5 / NA 29*54* (TAG→TAAytjAuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

CGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCA  >  NC_000913/4612172‑4612418
                                                                                                                     |                                                                                                                                 
cgccgcACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAAt                                                                                                              >  2:187806/1‑139 (MQ=255)
       cTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       <  2:1206468/139‑1 (MQ=255)
       cTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       <  1:187806/139‑1 (MQ=255)
                                  gCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            <  1:13472/139‑1 (MQ=255)
                                      cAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      >  1:1077985/1‑125 (MQ=255)
                                      cAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      <  2:1077985/125‑1 (MQ=255)
                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                                  >  2:521896/1‑89 (MQ=255)
                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                                  <  1:521896/89‑1 (MQ=255)
                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                  <  2:767490/137‑1 (MQ=255)
                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                  >  1:767490/1‑137 (MQ=255)
                                                                   gggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCttt                                                                              >  2:1488191/1‑104 (MQ=255)
                                                                   gggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCttt                                                                              <  1:1488191/104‑1 (MQ=255)
                                                                               ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaat                                                              >  2:1448732/1‑108 (MQ=255)
                                                                               ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaat                                                              <  1:1448732/108‑1 (MQ=255)
                                                                               ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcac                               >  1:1520493/1‑139 (MQ=255)
                                                                                    ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTTAGAAAAtt                                                                                                             <  1:996444/56‑1 (MQ=255)
                                                                                    ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAAtt                                                                                                             >  2:996444/1‑56 (MQ=255)
                                                                                    ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           <  2:372090/74‑1 (MQ=255)
                                                                                    ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           >  1:372090/1‑74 (MQ=255)
                                                                                    ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCAcaacaa                          >  1:1025841/1‑139 (MQ=255)
                                                                                          tttGTTCATGGGCCGAAAACGACCCTAAATTTCACACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAACAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAg                    <  1:1003310/139‑1 (MQ=255)
                                                                                            tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            <  2:516344/81‑1 (MQ=255)
                                                                                            tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            >  1:516344/1‑81 (MQ=255)
                                                                                                cATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGc              <  1:769078/139‑1 (MQ=255)
                                                                                                            aCGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCa  <  2:337440/139‑1 (MQ=255)
                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            >  1:543330/1‑62 (MQ=255)
                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            <  2:543330/62‑1 (MQ=255)
                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt                                          <  2:115039/96‑1 (MQ=255)
                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt                                          >  1:115039/1‑96 (MQ=255)
                                                                                                                     |                                                                                                                                 
CGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCA  >  NC_000913/4612172‑4612418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: