| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 835,596 | Δ1 bp | coding (349/963 nt) | ybiB → | putative family 3 glycosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 835,593 | 0 | G | . | 97.5% | 156.7 / ‑2.5 | 40 | coding (346/963 nt) | ybiB | putative family 3 glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (20/19); minor base C (1/0); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGGCGC > NC_000913/835463‑835726 | gCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCg < 1:544535/139‑1 (MQ=255) cagcagGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAAcc < 2:29200/139‑1 (MQ=255) cAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGc < 2:448869/139‑1 (MQ=255) atgccgatTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGctgactga > 1:1437782/1‑139 (MQ=255) aTCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTC‑CCGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGa > 2:218057/1‑139 (MQ=255) cccAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCAAAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATATTTGAAtt > 2:958125/1‑139 (MQ=255) aGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGAt < 1:218057/139‑1 (MQ=255) gTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg > 2:588315/1‑139 (MQ=255) ttACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATgg > 1:714351/1‑139 (MQ=255) gcgcgTAAATACGCCAACCTGACCCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGcc > 1:1298185/1‑139 (MQ=255) gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAc > 1:751642/1‑112 (MQ=255) gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAc < 2:751642/112‑1 (MQ=255) gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGcc > 2:1383292/1‑139 (MQ=255) cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg > 1:1598229/1‑127 (MQ=255) cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg < 2:1598229/127‑1 (MQ=255) cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCa > 2:463281/1‑139 (MQ=255) aaCAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTg < 1:588315/139‑1 (MQ=255) gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTg > 1:1270086/1‑89 (MQ=255) gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTg < 2:1270086/89‑1 (MQ=255) gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGct < 1:516707/91‑1 (MQ=255) gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGct > 2:516707/1‑91 (MQ=255) gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcgg < 2:1356526/139‑1 (MQ=255) ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggtt > 2:761236/1‑65 (MQ=255) ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggtt < 1:761236/67‑3 (MQ=255) ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcggc > 2:1121442/1‑139 (MQ=255) aaCCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcggcgg < 1:1121442/139‑1 (MQ=255) ttgttgGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcgcaggc > 2:326711/1‑139 (MQ=255) ttCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTcgac > 2:76093/1‑139 (MQ=255) tctcCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTcgacg < 1:1383292/139‑1 (MQ=255) aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 1:958125/139‑1 (MQ=255) aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 1:326711/139‑1 (MQ=255) aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa > 1:844773/1‑72 (MQ=255) aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa < 2:844773/72‑1 (MQ=255) aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg > 1:78832/1‑111 (MQ=255) aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg < 2:78832/111‑1 (MQ=255) tggtggTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTAt > 2:1237220/1‑69 (MQ=255) tggtggTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTAt < 1:1237220/69‑1 (MQ=255) gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 2:1480466/119‑1 (MQ=255) gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg > 1:1480466/1‑119 (MQ=255) gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCgg < 2:1432012/136‑1 (MQ=255) gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCgg > 1:1432012/1‑136 (MQ=255) gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGgcgc > 1:757381/1‑139 (MQ=255) | GCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGGCGC > NC_000913/835463‑835726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |