Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,022	(T)6→5	coding (68/1284 nt)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,017	0	T	.	100.0%	137.1 / NA	34	coding (63/1284 nt)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/18);  total (16/18)

ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACC  >  NC_000913/1868882‑1869139                                                                                                                                        |                                                                                                                           aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt                                                                                                                         >  1:1190485/1‑139 (MQ=255)  cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttt                                                                                                                        >  2:1170270/1‑139 (MQ=255)  cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttt                                                                                                                        >  1:605954/1‑139 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     >  2:730319/1‑136 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     <  2:592381/139‑4 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     <  1:730319/139‑4 (MQ=255)          cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     >  2:515268/1‑131 (MQ=255)          cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     <  1:515268/134‑4 (MQ=255)                 cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                        >  2:1019429/1‑139 (MQ=255)                           gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                              <  2:1190485/139‑1 (MQ=255)                                                    gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGCATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                        >  1:42986/1‑104 (MQ=255)                                                    gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     >  2:946676/1‑89 (MQ=255)                                                    gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                     <  1:946676/92‑4 (MQ=255)                                                    gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                        <  2:42986/104‑1 (MQ=255)                                                    gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                     <  2:605954/139‑1 (MQ=255)                                                                     ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                >  2:1178998/1‑111 (MQ=255)                                                                     ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                <  1:1178998/111‑1 (MQ=255)                                                                      gCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACg                                                   >  2:1351771/1‑139 (MQ=255)                                                                        atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAAc                                                                                             >  2:872034/1‑95 (MQ=255)                                                                        atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAAc                                                                                             <  1:872034/95‑1 (MQ=255)                                                                         tatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCg                                                >  1:1615247/1‑139 (MQ=255)                                                                                  gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                       <  2:1476765/60‑2 (MQ=255)                                                                                  gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                       >  1:1476765/1‑59 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                       >  1:622583/1‑134 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                       <  2:622583/134‑1 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                  <  2:1615247/139‑1 (MQ=255)                                                                                        tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                       <  1:131085/54‑2 (MQ=255)                                                                                        tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                       >  2:131085/1‑53 (MQ=255)                                                                                            cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                             >  2:809487/1‑139 (MQ=255)                                                                                                 tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                   <  2:549327/128‑1 (MQ=255)                                                                                                 tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                   >  1:549327/1‑128 (MQ=255)                                                                                                  cTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCATATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTcc                       <  1:1019429/139‑1 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                              <  2:27520/82‑1 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                              >  1:27520/1‑82 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                     <  1:1170270/139‑1 (MQ=255)                                                                                                        ggCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                 <  2:1566312/139‑1 (MQ=255)                                                                                                         gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                              >  2:1335372/1‑109 (MQ=255)                                                                                                         gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                              <  1:1335372/109‑1 (MQ=255)                                                                                                                     gCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                        >  2:287786/1‑103 (MQ=255)                                                                                                                     gCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                        <  1:287786/103‑1 (MQ=255)                                                                                                                        tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc  >  2:1110984/1‑138 (MQ=255)                                                                                                                        tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc  <  1:1110984/138‑1 (MQ=255)                                                                                                                         ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                             <  1:730/62‑1 (MQ=255)                                                                                                                         ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                             >  2:730/1‑62 (MQ=255)                                                                                                                               tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                 <  2:561459/52‑1 (MQ=255)                                                                                                                               tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                 >  1:561459/1‑52 (MQ=255)                                                                                                                               tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                       <  1:248965/94‑1 (MQ=255)                                                                                                                               tCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                       >  2:248965/1‑94 (MQ=255)                                                                                                                                        |                                                                                                                           ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACC  >  NC_000913/1868882‑1869139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑