Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,380,329	(G)5→6	coding (182/660 nt)	ycjU →	beta‑phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,380,324	1	.	G	100.0%	214.5 / NA	60	H59Q (CAC→CAG)	ycjU	beta‑phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (29/31);  total (29/31)

AATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑GGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCA  >  NC_000913/1380186‑1380456                                                                                                                                            |                                                                                                                                     aaTCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCGGCAGATTGCCGCTTAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAacac                                                                                                                                       <  2:1068807/139‑1 (MQ=255)      ccGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑ggggg                                                                                                                                 <  1:619605/139‑1 (MQ=255)               cgcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAgg                                                                                                                         >  1:385663/1‑139 (MQ=255)               cgcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAgg                                                                                                                         >  1:919745/1‑139 (MQ=255)               cgcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAgg                                                                                                                         >  2:723195/1‑139 (MQ=255)                     tGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACt                                                                                                                   >  2:1293483/1‑139 (MQ=255)                        aTTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTa                                                                                                                <  1:1236182/139‑1 (MQ=255)                            ccAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTc                                                                                                            >  2:1216408/1‑139 (MQ=255)                                 cGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAgg                                                                                                       >  2:1323738/1‑139 (MQ=255)                                   tGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGgag                                                                                                     >  2:1246638/1‑139 (MQ=255)                                     gcagcaGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGagag                                                                                                   >  1:557383/1‑139 (MQ=255)                                       agcagATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAggg                                                                                                 <  1:1461769/139‑1 (MQ=255)                                              tGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAAc                                                                                                              >  2:1290031/1‑119 (MQ=255)                                              tGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAAc                                                                                                              <  1:1290031/119‑1 (MQ=255)                                                   cTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGt                                                                                     <  2:1564513/139‑1 (MQ=255)                                                         ttGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCa                                                                               <  1:723195/139‑1 (MQ=255)                                                              aTCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCACAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          >  1:762264/1‑139 (MQ=255)                                                              aTCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCACAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          <  2:762264/139‑1 (MQ=255)                                                                    aTTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                                    <  2:18347/139‑1 (MQ=255)                                                                      tGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTa                                                                  >  2:811915/1‑139 (MQ=255)                                                                       gATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGGGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTAt                                                                 <  1:1216979/139‑1 (MQ=255)                                                                        aTGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          >  1:597039/1‑129 (MQ=255)                                                                        aTGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          <  2:597039/129‑1 (MQ=255)                                                                        aTGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATg                                                                <  2:919745/139‑1 (MQ=255)                                                                             cAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCcac                                                           >  2:1356509/1‑139 (MQ=255)                                                                              aGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCcact                                                          <  1:811915/139‑1 (MQ=255)                                                                                  tAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCca                                                            <  2:1571440/133‑1 (MQ=255)                                                                                  tAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCca                                                            >  1:1571440/1‑133 (MQ=255)                                                                                   aaCGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTa                                                     >  1:138708/1‑139 (MQ=255)                                                                                       aaTCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTAcgcg                                                 <  1:1356509/139‑1 (MQ=255)                                                                                          cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                        >  1:383019/1‑65 (MQ=255)                                                                                          cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                        <  2:383019/65‑1 (MQ=255)                                                                                          cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACtt                                                                                                                  >  2:1342493/1‑71 (MQ=255)                                                                                          cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACtt                                                                                                                  <  1:1342493/71‑1 (MQ=255)                                                                                                 aGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGTGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGt                                       <  1:471255/139‑1 (MQ=255)                                                                                                    gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAGAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCg                                                                                      >  1:272613/1‑89 (MQ=255)                                                                                                    gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAag                                                                                                                            >  2:406629/1‑51 (MQ=255)                                                                                                    gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAag                                                                                                                            <  1:406629/51‑1 (MQ=255)                                                                                                    gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCg                                                                                      <  2:272613/89‑1 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAggg                                                                                                 >  1:1585514/1‑77 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAggg                                                                                                 <  2:1585514/77‑1 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATc                                                                         <  2:1257283/101‑1 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATc                                                                         >  1:1257283/1‑101 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                                    <  1:1358602/106‑1 (MQ=255)                                                                                                     aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                                    >  2:1358602/1‑106 (MQ=255)                                                                                                      tCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACg                                  <  1:1293483/139‑1 (MQ=255)                                                                                                       cAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑gg                                                                                                                                    >  2:931629/1‑39 (MQ=255)                                                                                                       cAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑gg                                                                                                                                    <  1:931629/39‑1 (MQ=255)                                                                                                         gCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTa                                                                                                                >  2:1579721/1‑58 (MQ=255)                                                                                                         gCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTa                                                                                                                <  1:1579721/58‑1 (MQ=255)                                                                                                         gCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          >  1:645426/1‑96 (MQ=255)                                                                                                         gCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                          <  2:645426/96‑1 (MQ=255)                                                                                                                    tctcTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAAc                                                                                                              <  2:102274/49‑1 (MQ=255)                                                                                                                    tctcTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAAc                                                                                                              >  1:102274/1‑49 (MQ=255)                                                                                                                       ctGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCAtt                 <  2:385663/139‑1 (MQ=255)                                                                                                                        tGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATc                                                                         >  2:1160426/1‑82 (MQ=255)                                                                                                                        tGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATc                                                                         <  1:1160426/82‑1 (MQ=255)                                                                                                                            gcgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGt                                       >  2:239920/1‑112 (MQ=255)                                                                                                                            gcgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGt                                       <  1:239920/112‑1 (MQ=255)                                                                                                                             cgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGctct           <  1:1468056/139‑1 (MQ=255)                                                                                                                                 ttCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                                    >  2:1418464/1‑78 (MQ=255)                                                                                                                                 ttCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                                    <  1:1418464/78‑1 (MQ=255)                                                                                                                                  tCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGCt      >  2:493484/1‑139 (MQ=255)                                                                                                                                  tCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTC‑‑‑‑TCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCa  <  1:739759/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     AATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑GGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCA  >  NC_000913/1380186‑1380456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑