| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,240,754 | (C)5→6 | coding (915/1041 nt) | galS ← | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,240,749 | 1 | . | C | 96.6% | 201.0 / ‑0.5 | 59 | coding (920/1041 nt) | galS | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (25/32); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATG > NC_000913/2240611‑2240873 | tcacAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg < 1:736500/139‑1 (MQ=255) cacaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCtgctg‑c > 1:1027465/1‑139 (MQ=255) acaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cc < 2:1273226/139‑1 (MQ=255) aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑c < 1:1371793/139‑1 (MQ=255) aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccc > 1:673719/1‑138 (MQ=255) tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctg < 1:1088655/139‑4 (MQ=255) tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctg > 1:514613/1‑136 (MQ=255) gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCa > 2:176467/1‑139 (MQ=255) aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg < 1:35188/139‑1 (MQ=255) ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTgg > 2:1363511/1‑139 (MQ=255) tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGc < 2:1027465/139‑1 (MQ=255) tGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg < 1:337849/139‑1 (MQ=255) tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 2:671568/139‑1 (MQ=255) tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 1:176467/139‑1 (MQ=255) cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 1:1392191/133‑1 (MQ=255) cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 2:1392191/1‑133 (MQ=255) cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAAt > 2:899588/1‑139 (MQ=255) ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg > 2:532691/1‑139 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:532691/139‑1 (MQ=255) gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 1:1253088/1‑118 (MQ=255) gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 2:1253088/118‑1 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAg > 1:1247550/1‑84 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAg < 2:1247550/84‑1 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt < 2:1120231/103‑1 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCCGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt > 1:1120231/1‑103 (MQ=255) gACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg < 1:45224/139‑1 (MQ=255) cAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCg < 2:514613/139‑1 (MQ=255) aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt < 2:929447/139‑1 (MQ=255) aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt < 2:1587822/139‑1 (MQ=255) aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCTCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt > 1:693433/1‑139 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 1:84401/139‑1 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 1:27047/139‑1 (MQ=255) tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt > 1:1565358/1‑137 (MQ=255) tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt < 2:1565358/137‑1 (MQ=255) tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg > 2:681829/1‑139 (MQ=255) gACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGgtgt > 2:1244010/1‑139 (MQ=255) aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa < 1:1202721/139‑1 (MQ=255) gCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAAc > 2:1451375/1‑139 (MQ=255) cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt < 1:1244010/139‑1 (MQ=255) cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCTCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt < 2:693433/139‑1 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg < 2:620214/82‑1 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg > 1:620214/1‑82 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:748875/100‑1 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 1:748875/1‑100 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGaa > 2:1178367/1‑139 (MQ=255) aTAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAata < 1:1363511/139‑1 (MQ=255) tggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg < 2:67768/52‑1 (MQ=255) tggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg > 1:67768/1‑52 (MQ=255) ggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAc < 1:1178367/139‑1 (MQ=255) ggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat > 2:295523/1‑139 (MQ=255) cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGTCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg > 2:1187652/1‑84 (MQ=255) cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGTCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg < 1:1187652/84‑1 (MQ=255) ggATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 1:110034/139‑1 (MQ=255) aaTATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATg > 1:559506/1‑139 (MQ=255) ttGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:550467/68‑1 (MQ=255) ttGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:550467/1‑68 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 1:326489/54‑1 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa > 2:326489/1‑54 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:45698/65‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:45698/1‑65 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg > 1:1068516/1‑94 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg < 2:1068516/94‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa < 2:429484/114‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa > 1:429484/1‑114 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga < 2:700033/126‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga > 1:700033/1‑126 (MQ=255) tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg < 1:904983/65‑1 (MQ=255) tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg > 2:904983/1‑65 (MQ=255) tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 2:690861/125‑1 (MQ=255) tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag > 1:690861/1‑125 (MQ=255) | TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATG > NC_000913/2240611‑2240873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |