Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,285,401	+G	coding (1026/1761 nt)	yejM →	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,285,397	1	.	G	100.0%	212.8 / NA	59	L341R (CTG→CGG)	yejM	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (33/26);  total (33/26)

GCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCT  >  NC_000913/2285271‑2285523                                                                                                                                |                                                                                                                               gCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATtct                                                                                                                     >  2:967590/1‑139 (MQ=255)       cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                               <  1:967590/139‑1 (MQ=255)       cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                               <  2:253986/139‑1 (MQ=255)       cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                               <  1:1561608/139‑1 (MQ=255)                aCGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑gggg                                                                                                                            >  2:1220292/1‑116 (MQ=255)                aCGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑gggg                                                                                                                            <  1:1220292/116‑1 (MQ=255)                                 ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGc                                                                                     >  1:642380/1‑139 (MQ=255)                                 ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGc                                                                                     >  2:179718/1‑139 (MQ=255)                                 ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                           >  2:452325/1‑99 (MQ=255)                                 ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                           <  1:452325/100‑2 (MQ=255)                                      tGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGc                                                                                >  2:813062/1‑139 (MQ=255)                                      tGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGc                                                                                >  1:144007/1‑139 (MQ=255)                                      tGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAACTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGcc                                                                                    >  1:1413851/1‑135 (MQ=255)                                      tGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAACTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGcc                                                                                    <  2:1413851/135‑1 (MQ=255)                                        gCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAt                                                                              <  2:30927/139‑1 (MQ=255)                                         cATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                           <  2:169958/92‑2 (MQ=255)                                         cATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                           >  1:169958/1‑91 (MQ=255)                                           tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCt                                                                                                                                 <  1:244227/85‑1 (MQ=255)                                           tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCt                                                                                                                                 >  2:244227/1‑85 (MQ=255)                                           tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                               >  2:1406362/1‑103 (MQ=255)                                           tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                               <  1:1406362/103‑1 (MQ=255)                                            ctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgtt                                                                          >  2:1376167/1‑139 (MQ=255)                                             tcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTa                                                                                                      <  2:894948/110‑1 (MQ=255)                                             tcGCCGAGCTATATGGACAGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTa                                                                                                      >  1:894948/1‑110 (MQ=255)                                                               ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCg                                                       >  1:902269/1‑139 (MQ=255)                                                                  aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGccc                                                                                               <  2:1443971/96‑1 (MQ=255)                                                                  aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGccc                                                                                               >  1:1443971/1‑96 (MQ=255)                                                                    tCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                           >  2:1304385/1‑82 (MQ=255)                                                                    tCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                           <  1:1304385/82‑1 (MQ=255)                                                                    tCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGt                                                  >  1:534320/1‑139 (MQ=255)                                                                      tGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATtct                                                                                                                     <  2:980800/70‑1 (MQ=255)                                                                      tGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATtct                                                                                                                     >  1:980800/1‑70 (MQ=255)                                                                                tACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATc                                      >  2:1401957/1‑139 (MQ=255)                                                                                  cGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCcg                                    >  1:1046054/1‑139 (MQ=255)                                                                                    ccTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCcgac                                  <  2:642380/139‑1 (MQ=255)                                                                                      tGCGGCATTAATTACTGCGCTTAATCAGCGAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGcc                                                                                                >  1:882109/1‑75 (MQ=255)                                                                                      tGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGcc                                                                                                <  2:882109/75‑1 (MQ=255)                                                                                       gCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAg                               >  2:1567164/1‑139 (MQ=255)                                                                                         ggCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCa                             <  1:1376167/139‑1 (MQ=255)                                                                                            attaattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGAc                          <  1:1567164/139‑1 (MQ=255)                                                                                                 ttaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCa                     <  1:179718/139‑1 (MQ=255)                                                                                                 ttaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCa                     <  2:534320/139‑1 (MQ=255)                                                                                                   aCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACg                   <  2:1046054/139‑1 (MQ=255)                                                                                                     tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCa                 >  1:503253/1‑139 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGccc                                                                                               >  2:1585586/1‑59 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGccc                                                                                               <  1:1585586/59‑1 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAg                                                     >  1:781817/1‑101 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAg                                                     <  2:781817/101‑1 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGt               >  2:1422975/1‑139 (MQ=255)                                                                                                       cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGt               >  1:1451091/1‑139 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGc                                                                                <  1:441331/72‑1 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGc                                                                                >  2:441331/1‑72 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCa                                             >  2:1056289/1‑107 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCa                                             <  1:1056289/107‑1 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTgg             >  2:229041/1‑139 (MQ=255)                                                                                                         cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTgg             >  2:464879/1‑139 (MQ=255)                                                                                                          ttAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCt                                                                                            >  1:914434/1‑59 (MQ=255)                                                                                                          ttAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCt                                                                                            <  2:914434/59‑1 (MQ=255)                                                                                                          ttAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGa                                                      >  2:871245/1‑97 (MQ=255)                                                                                                          ttAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGa                                                      <  1:871245/97‑1 (MQ=255)                                                                                                           tAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAg                                                                                                  >  2:41929/1‑52 (MQ=255)                                                                                                           tAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAg                                                                                                  <  1:41929/52‑1 (MQ=255)                                                                                                            aaTCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGa                           >  2:1385063/1‑122 (MQ=255)                                                                                                            aaTCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGa                           <  1:1385063/122‑1 (MQ=255)                                                                                                                  cAAGGCTATCAGCTGGGGGTTATTCTTATCAGATGGCTTTa                                                                                                      <  2:980594/41‑1 (MQ=255)                                                                                                                  cAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTa                                                                                                      >  1:980594/1‑41 (MQ=255)                                                                                                                    aGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAActggct  >  2:1237424/1‑139 (MQ=255)                                                                                                                                |                                                                                                                               GCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCT  >  NC_000913/2285271‑2285523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑