| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,728,118 | (C)8→10 | coding (1254/1455 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 83.3% | 98.6 / 13.4 | 36 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (2/4); new base C (13/17); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,728,110 | 2 | . | C | 83.3% | 96.9 / 13.4 | 36 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (2/4); new base C (13/17); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA > NC_000913/3727972‑3728236 || ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑t > 2:126774/1‑139 (MQ=255)ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑t > 1:1014930/1‑139 (MQ=255) tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccc < 1:1407991/139‑1 (MQ=255) gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACG‑‑T‑‑cccc < 1:774809/139‑1 (MQ=255) aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑cccccccc < 2:35847/139‑1 (MQ=255) ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccg > 2:1437368/1‑136 (MQ=255) gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTaa < 1:600631/139‑1 (MQ=255) gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAg < 2:1345277/139‑1 (MQ=255) cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAgc > 1:461266/1‑139 (MQ=255) gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGGGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAg < 1:535495/139‑1 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccg < 1:1034215/122‑4 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccg > 2:1034215/1‑119 (MQ=255) ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc < 2:461266/139‑1 (MQ=255) aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGAcc > 2:444991/1‑139 (MQ=255) cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAgctgcgcattatggc > 1:993574/1‑129 (MQ=255) aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAgctgct < 2:82141/119‑1 (MQ=255) aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAgctgct > 1:82141/1‑119 (MQ=255) ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt < 1:1437368/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGCACTCGAACCGCTGACCGCTGATCCCCGCCacctcccc > 2:1204059/1‑132 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccgtac < 1:1576309/99‑7 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccgtac > 2:1576309/1‑93 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGa > 2:1149813/1‑107 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGa < 1:1149813/107‑1 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGata > 2:1129996/1‑123 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata < 1:1129996/123‑1 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCCTACCGTAAGCGAGCTTCCGACCGCTGATTTCCCCCCGCATCTgcc > 2:672409/1‑137 (MQ=255) tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg < 2:131570/139‑1 (MQ=255) tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg > 1:131570/1‑139 (MQ=255) gCGGCAACAAATCAATGAGCGATTTCTCTGGAATCGCCGCCATCTGCGCCAGCCTTGCCGCGCCCAGGGCTGGCCCCACAACC‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 1:454416/139‑1 (MQ=255) gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 2:989509/1‑133 (MQ=255) gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 1:989509/133‑1 (MQ=255) caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt < 1:444991/139‑1 (MQ=255) gtcGATTTCTCTCGATTCGCCCCGATCTTCGCCAGCCTTGCTTCGCCCAGTGCTGGCCCCACA‑‑C‑‑CCGCCCCCGTACGGTAATCTAGCTGCTGACCGCTGATATCCACCAGCATCTGACGCCAGTACTCACTACgcgcac < 1:329907/137‑3 (MQ=255) ttcgcaggATTCGTCGTGATCTTCGGCAGCCTTGTTGCGCCCAGTGGAGGCCCCACA‑‑TCCCCCCCCCCGTCCGGTAATCGTGCTGCTGACCGCTAATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc < 1:126774/133‑1 (MQ=255) ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:249120/139‑1 (MQ=255) ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:1014930/139‑1 (MQ=255) aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa < 2:1452502/110‑1 (MQ=255) aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTAAGGTAATCGAGCTGCTGACCGCTGAAATCCGCCAGCATCTGACGCCa > 1:1452502/1‑110 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccgta > 1:380226/1‑54 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑ccccccccccgta < 2:380226/59‑6 (MQ=255) ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt > 2:120464/1‑107 (MQ=255) ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt < 1:120464/107‑1 (MQ=255) gCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTaa < 1:37533/49‑1 (MQ=255) gCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTaa > 2:37533/1‑49 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 1:581276/84‑1 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 2:581276/1‑84 (MQ=255) ccAGTGCTGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa < 1:1204059/139‑1 (MQ=255) tGGCCCCACA‑‑TCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa < 1:672409/139‑1 (MQ=255) gCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt < 2:787295/115‑1 (MQ=255) gCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt > 1:787295/1‑115 (MQ=255) || GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑T‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA > NC_000913/3727972‑3728236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |