Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,757,753	Δ5 bp	coding (969‑973/1005 nt)	ldtE ←	murein L,D‑transpeptidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1757753	1757757	5	52 [0]	[0] 52	ldtE	murein L,D‑transpeptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1757752	0 (0.000)	50 (0.790)	38/206	0.5	100%	coding (974/1005 nt)	ldtE	murein L,D‑transpeptidase
	?	NC_000913	1757758 =	0 (0.000)					coding (968/1005 nt)	ldtE	murein L,D‑transpeptidase

CATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1757633‑1757752 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTT  >  NC_000913/1757758‑1757894                                                                                                                                                                                                                                                                   CATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACT                                                                                                                        >  2:979033/1‑139              TACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGC                                                                                                           >  2:63562/1‑139                   CAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGC                                                                                                      >  1:663520/1‑139                             TACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTG                                                                                                <  1:856916/135‑1                             TACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATGCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTG                                                                                                >  2:856916/1‑135                              ACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                            >  2:656320/1‑138                              ACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGG                                                                                            <  1:656320/138‑1                               CCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAG                                                                                          <  1:63562/139‑1                               CCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACT                                                                                                                        <  2:507645/109‑1                               CCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACT                                                                                                                        >  1:507645/1‑109                                CTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGT                                                                                         >  1:1031872/1‑139                                CTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                            >  1:1091944/1‑104                                CTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                            <  2:1091944/104‑1                                  GTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                            <  1:200977/102‑1                                  GTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCT                                                                                                                            >  2:200977/1‑102                                       GTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCA                                                                                  >  1:1116601/1‑139                                                  CGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGA                                                                       <  1:263582/139‑1                                                  CGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTG                                                                                                                  >  1:1014861/1‑96                                                  CGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTG                                                                                                                  <  2:1014861/96‑1                                                           TGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATA                                                              >  1:1462208/1‑139                                                           TGCGCCA‑TTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATAC                                                             >  2:1377481/1‑139                                                             CGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGG                                                                                                   >  1:211486/1‑100                                                             CGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGG                                                                                                   <  2:211486/100‑1                                                             CGCCATTTTTTTTGTCTGCCGTTTACCGTTACCGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAG                                                                                          >  2:723993/1‑109                                                             CGCCATTTTTTTTGTCTGCCGTTTACCGTTACCGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAG                                                                                          <  1:723993/109‑1                                                                 ATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCG                                                                                                                              <  2:1088863/69‑1                                                                 ATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCG                                                                                                                              >  1:1088863/1‑69                                                                   TTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCC                                                      >  1:1219538/1‑139                                                                                     ACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTAT                                    <  1:1377481/139‑1                                                                                     ACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTAT                                    <  2:1462208/139‑1                                                                                        gtTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGA                                 <  2:1031872/137‑1                                                                                         tTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGAC                                >  2:1488559/2‑139                                                                                         tTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGC                                                                                                                             <  2:1468146/45‑1                                                                                         tTACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGC                                                                                                                             >  1:1468146/2‑46                                                                                          TACTGCGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACT                               <  2:1116601/139‑1                                                                                            CTGCGTCACGCGTAACATATTCTCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                         <  2:86529/111‑1                                                                                            CTGCGTCACGCGTAACATATTCTCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG                                                         >  1:86529/1‑111                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTT                          >  2:205427/1‑139                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTT                          <  2:663520/139‑1                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTG                                                                                       >  2:1096232/1‑78                                                                                               CGTCACGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTG                                                                                       <  1:1096232/78‑1                                                                                                    CGCGTAACATATTCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGAT                     >  2:869189/1‑139                                                                                                                TCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTA          >  1:1363759/1‑138                                                                                                                TCCCTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTA          <  2:1363759/138‑1                                                                                                                   CTTGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGT      >  2:1240721/1‑139                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCT    >  2:1489783/1‑139                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCT    >  2:1089756/1‑139                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCT                                                                                                     >  2:394143/1‑42                                                                                                                     TGCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCT                                                                                                     <  1:394143/42‑1                                                                                                                      GCTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCC‑TATTGTCTTT  >  2:94782/1‑139                                                                                                                        TTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGA                                 <  2:593021/107‑1                                                                                                                        TTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGA                                 >  1:593021/1‑107                                                                                                                                                                                                                                                                   CATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1757633‑1757752 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTT  >  NC_000913/1757758‑1757894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.