Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,765	0	C	T	100.0%	166.5 / NA	51	intergenic (+134/‑58)	kefC/folA	potassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NC_000913/49637‑49896                                                                                                                                 |                                                                                                                                    agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           >  2:50258/1‑139 (MQ=255)      cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  1:353271/139‑1 (MQ=255)         aaaTCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                                                                                   <  1:634062/139‑1 (MQ=255)                 cTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgac                                                                                                                                  >  1:1351278/1‑116 (MQ=255)                 cTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgac                                                                                                                                  <  2:1351278/116‑1 (MQ=255)                    tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTgg                                                                                                        >  2:1249896/1‑139 (MQ=255)                                  tgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttttt                                                                                          <  2:382961/139‑1 (MQ=255)                                        tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCggg                                                                                    >  1:101479/1‑139 (MQ=255)                                         gtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                   <  2:101479/139‑1 (MQ=255)                                          tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  1:171356/103‑1 (MQ=255)                                          tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  2:171356/1‑103 (MQ=255)                                          tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa                                                                                  >  2:467446/1‑139 (MQ=255)                                          tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa                                                                                  >  2:140377/1‑139 (MQ=255)                                              aTTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  1:1271615/100‑1 (MQ=255)                                              aTTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     >  2:1271615/1‑100 (MQ=255)                                                 ggaaTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTGTTTTTATCGGGAAATCTCaa                                                                           <  1:948973/137‑1 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:680139/94‑1 (MQ=255)                                                    aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     >  1:680139/1‑94 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  2:1193143/1‑90 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  1:1193143/90‑1 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                   <  2:1457219/125‑1 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                   >  1:1457219/1‑125 (MQ=255)                                                       cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                                                                     >  2:368826/1‑139 (MQ=255)                                                                  gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                                 <  1:845951/116‑1 (MQ=255)                                                                  gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                                 >  2:845951/1‑116 (MQ=255)                                                                  gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTgcg                                                          <  1:1249896/139‑1 (MQ=255)                                                                      gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  2:855348/139‑1 (MQ=255)                                                                         agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAg                                                   >  2:1581554/1‑139 (MQ=255)                                                                           cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                                                                                       >  2:223982/1‑85 (MQ=255)                                                                           cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                                                                                       <  1:223982/85‑1 (MQ=255)                                                                             tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  1:157327/1‑68 (MQ=255)                                                                             tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  2:157327/68‑1 (MQ=255)                                                                                   cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATcg                                         >  2:125006/1‑139 (MQ=255)                                                                                     ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     >  1:935546/1‑61 (MQ=255)                                                                                     ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:935546/61‑1 (MQ=255)                                                                                         cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                   <  1:1581554/139‑1 (MQ=255)                                                                                                      tttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGc                                                  <  2:1605604/111‑1 (MQ=255)                                                                                                      tttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGc                                                  >  1:1605604/1‑111 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     >  1:484521/1‑42 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:484521/42‑1 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGa                                                                                                     <  1:263268/58‑1 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGa                                                                                                     >  2:263268/1‑58 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt                             >  2:203694/1‑130 (MQ=255)                                                                                                        tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAGCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt                             <  1:203694/130‑1 (MQ=255)                                                                                                           tCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt                 <  1:467446/139‑1 (MQ=255)                                                                                                               catcatCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg             >  1:1554858/1‑139 (MQ=255)                                                                                                                         gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  2:1238453/88‑1 (MQ=255)                                                                                                                         gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      >  1:1238453/1‑88 (MQ=255)                                                                                                                          cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                       <  2:1222270/70‑1 (MQ=255)                                                                                                                          cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                       >  1:1222270/1‑70 (MQ=255)                                                                                                                          cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                   <  2:1164023/106‑1 (MQ=255)                                                                                                                          cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                   >  1:1164023/1‑106 (MQ=255)                                                                                                                          cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc  >  2:922615/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                    AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NC_000913/49637‑49896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑