Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,463	T→C	V683A (GTG→GCG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,463	0	T	C	100.0%	196.4 / NA	54	V683A (GTG→GCG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/27);  total (27/27)

TTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACG  >  NC_000913/522330‑522596                                                                                                                                      |                                                                                                                                      ttCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                                  <  2:114202/139‑1 (MQ=255) ttCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                                  <  1:1576732/139‑1 (MQ=255)                   aaCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGGAGCGATGGTGGTACTGATCACCGc                                                                                                                  <  2:985110/137‑1 (MQ=255)                   aaCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                  >  1:985110/1‑137 (MQ=255)                    aCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTg                                                                                                               <  2:199359/139‑1 (MQ=255)                       gCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCgg                                                                                                            >  2:484561/1‑139 (MQ=255)                           gTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtg                                                                                                        >  1:1113675/1‑139 (MQ=255)                                cacaACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                   <  2:1273843/139‑1 (MQ=255)                                     cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                              >  1:569250/1‑139 (MQ=255)                                     cTCAACCGCCAGTTCCCGACCATTAGCCCGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                              >  2:405693/1‑139 (MQ=255)                                      tCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                             <  2:569250/139‑1 (MQ=255)                                             ccAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcag                                                                                      >  2:1070196/1‑139 (MQ=255)                                                   cccGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtgcag                                                                                <  2:936236/139‑1 (MQ=255)                                                           ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                             <  1:273302/86‑1 (MQ=255)                                                           ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                             >  2:273302/1‑86 (MQ=255)                                                           ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAAt                                                                        >  1:209816/1‑139 (MQ=255)                                                                 tGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtca                                                                  <  2:933163/139‑1 (MQ=255)                                                                         atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                   >  1:358303/1‑114 (MQ=255)                                                                         atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                   <  2:358303/114‑1 (MQ=255)                                                                          ttGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatca                                                         <  2:209816/139‑1 (MQ=255)                                                                           tGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                          >  2:1196428/1‑105 (MQ=255)                                                                           tGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                          <  1:1196428/105‑1 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                            <  2:1315133/69‑1 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                            >  1:1315133/1‑69 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                  <  1:1194515/79‑1 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                  >  2:1194515/1‑79 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                      >  2:774293/1‑139 (MQ=255)                                                                             gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                      >  1:2299/1‑139 (MQ=255)                                                                                           cAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGAGgc                                        >  2:1081595/1‑139 (MQ=255)                                                                                            aGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACGGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcg                                       <  1:1021278/139‑1 (MQ=255)                                                                                            aGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcg                                       <  1:774293/139‑1 (MQ=255)                                                                                             ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTg                                                                                                               <  2:418822/66‑1 (MQ=255)                                                                                             ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTg                                                                                                               >  1:418822/1‑66 (MQ=255)                                                                                             ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                      >  2:88015/1‑139 (MQ=255)                                                                                              gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                             <  2:1555026/131‑1 (MQ=255)                                                                                              gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                             >  1:1555026/1‑131 (MQ=255)                                                                                              gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                     >  1:490125/1‑139 (MQ=255)                                                                                                cggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGcaca                                   >  1:495742/1‑139 (MQ=255)                                                                                                  gtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGcac                                                                                        >  1:257322/1‑84 (MQ=255)                                                                                                  gtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGcac                                                                                        <  2:257322/84‑1 (MQ=255)                                                                                                  gtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                                 <  2:2299/139‑1 (MQ=255)                                                                                                     aGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtg                                                  >  2:1446493/1‑119 (MQ=255)                                                                                                     aGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtg                                                  <  1:1446493/119‑1 (MQ=255)                                                                                                                     aaGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActg              >  2:241171/1‑139 (MQ=255)                                                                                                                       gTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct            <  2:495742/139‑1 (MQ=255)                                                                                                                        tCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                          >  1:695711/1‑60 (MQ=255)                                                                                                                        tCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                          <  2:695711/60‑1 (MQ=255)                                                                                                                         cGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACtgctgc          <  1:484561/139‑1 (MQ=255)                                                                                                                          gggCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                   <  2:120696/49‑1 (MQ=255)                                                                                                                          gggCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                   >  1:120696/1‑49 (MQ=255)                                                                                                                           ggCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGt        >  1:255582/1‑139 (MQ=255)                                                                                                                            gcgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAATCTGCTGCGTa       <  1:405693/139‑1 (MQ=255)                                                                                                                             cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAc      >  1:592940/1‑139 (MQ=255)                                                                                                                                 ggAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACg  <  2:1342865/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACG  >  NC_000913/522330‑522596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑