Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 540,515 C→T intergenic (+7/‑50) allB → / → ybbY allantoinase/putative uracil/xanthine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913540,5150CT100.0% 137.9 / NA 43intergenic (+7/‑50)allB/ybbYallantoinase/putative uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/20);  total (23/20)

TGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACAC  >  NC_000913/540388‑540638
                                                                                                                               |                                                                                                                           
tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  <  2:741365/139‑1 (MQ=255)
tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  <  1:588275/139‑1 (MQ=255)
tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  <  1:544223/139‑1 (MQ=255)
       aCCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCtt                                                                                                           >  1:314509/1‑139 (MQ=255)
                                ccATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTgcgg                                                                                                       <  1:907580/118‑1 (MQ=255)
                                ccATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTgcgg                                                                                                       >  2:907580/1‑118 (MQ=255)
                                 cATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg                                                                                 <  2:314509/139‑1 (MQ=255)
                                    cTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtgtgt                                                                              <  1:842135/139‑1 (MQ=255)
                                        cGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtctc                                                                                            <  1:369814/121‑1 (MQ=255)
                                        cGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtctc                                                                                            >  2:369814/1‑121 (MQ=255)
                                            gtgatgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTc                                                                      >  1:250212/1‑139 (MQ=255)
                                                      ttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTg                                                                                                                         >  1:661884/1‑78 (MQ=255)
                                                      ttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTg                                                                                                                         <  2:661884/78‑1 (MQ=255)
                                                       tACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGt                                                           >  1:697335/1‑139 (MQ=255)
                                                         cGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCa                                                         >  1:848928/1‑139 (MQ=255)
                                                         cGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCa                                                         >  1:1458838/1‑139 (MQ=255)
                                                              ttGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgc                                                    >  1:459906/1‑139 (MQ=255)
                                                              ttGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgc                                                    >  1:139044/1‑139 (MQ=255)
                                                                      ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                 >  1:171745/1‑86 (MQ=255)
                                                                      ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                 <  2:171745/86‑1 (MQ=255)
                                                                        cTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGt                                          >  1:316875/1‑139 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  >  2:845114/1‑64 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  <  1:845114/64‑1 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTgc                                                                                                         <  1:6963/73‑1 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTgc                                                                                                         >  2:6963/1‑73 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTAt                                       >  2:94740/1‑139 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTAt                                       >  2:320303/1‑139 (MQ=255)
                                                                                ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGt                                                                                        <  2:1543913/85‑1 (MQ=255)
                                                                                ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGt                                                                                        >  1:1543913/1‑85 (MQ=255)
                                                                                   cgcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTg                                                                                                                         >  1:787270/1‑49 (MQ=255)
                                                                                   cgcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTg                                                                                                                         <  2:787270/49‑1 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  >  1:151778/1‑54 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                  <  2:151778/54‑1 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtctc                                                                                            <  2:390085/76‑1 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtctc                                                                                            >  1:390085/1‑76 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtc                                                                                              <  2:414580/74‑1 (MQ=255)
                                                                                     ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtc                                                                                              >  1:414580/1‑74 (MQ=255)
                                                                                                   tATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTa               <  2:697335/139‑1 (MQ=255)
                                                                                                       cTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTaa                                                                                     >  1:1546353/1‑65 (MQ=255)
                                                                                                       cTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTaa                                                                                     <  2:1546353/65‑1 (MQ=255)
                                                                                                           aaCATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCTGGCATtc                                                                                              <  1:1054510/52‑1 (MQ=255)
                                                                                                           aaCATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGCCGGGCATtc                                                                                              >  2:1054510/1‑52 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac  <  2:139044/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                           
TGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACAC  >  NC_000913/540388‑540638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: