Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,102	C→T	*363* (TAG→TAA)	rlpA ←	septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,102	0	C	T	100.0%	231.5 / NA	71	*363* (TAG→TAA)	rlpA	septal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/31);  total (40/31)

GAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCG  >  NC_000913/663969‑664233                                                                                                                                      |                                                                                                                                    gAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTg                                                                                                                                <  2:1158858/139‑1 (MQ=255)  aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  2:725872/1‑139 (MQ=255)  aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               <  2:521351/139‑1 (MQ=255)        gACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgta                                                                                                                         >  1:914362/1‑139 (MQ=255)                tGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTCGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  2:859809/1‑125 (MQ=255)                tGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTCGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               <  1:859809/125‑1 (MQ=255)                     gAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                                        >  1:1056864/1‑127 (MQ=255)                     gAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                                        <  2:1056864/127‑1 (MQ=255)                                 tGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCtt                                                                                                <  1:725872/139‑1 (MQ=255)                                 tGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCtt                                                                                                <  2:1312378/139‑1 (MQ=255)                                  gCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTc                                                                                               >  2:356260/1‑139 (MQ=255)                                              cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACg                                                                                   >  1:1061824/1‑139 (MQ=255)                                               aaaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                  <  1:356260/139‑1 (MQ=255)                                                aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGtt                                                                                 >  2:933426/1‑139 (MQ=255)                                                aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATAGGGCTCCGGTTTGCAAACGtt                                                                                 >  1:778942/1‑139 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGGTTTACGTTACTgcg                                                                                                                              >  1:1020342/1‑92 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:1020342/92‑1 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:654423/92‑1 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:105561/92‑1 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  1:654423/1‑92 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  1:105561/1‑92 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            >  2:469203/1‑94 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            <  1:469203/94‑1 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  2:1401734/1‑139 (MQ=255)                                                  tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  2:1092590/1‑139 (MQ=255)                                                   gCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                              >  2:1515188/1‑139 (MQ=255)                                                   gCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                              >  2:800967/1‑139 (MQ=255)                                                     aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  2:1396913/1‑88 (MQ=255)                                                     aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               <  1:1396913/88‑1 (MQ=255)                                                      cTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  1:1401734/139‑1 (MQ=255)                                                      cTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  2:1061824/139‑1 (MQ=255)                                                       tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaataa                                                                                                                  >  1:536059/1‑99 (MQ=255)                                                       tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaataa                                                                                                                  <  2:536059/99‑1 (MQ=255)                                                        aCCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          <  2:792417/90‑1 (MQ=255)                                                        aCCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          >  1:792417/1‑90 (MQ=255)                                                          cGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:1191912/84‑1 (MQ=255)                                                          cGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  1:1191912/1‑84 (MQ=255)                                                            gcaggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTa                                                                                                                                   >  1:1559100/1‑77 (MQ=255)                                                            gcaggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTa                                                                                                                                   <  2:1559100/77‑1 (MQ=255)                                                                   tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTg                                                                                                                                <  1:1535483/73‑1 (MQ=255)                                                                   tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTg                                                                                                                                >  2:1535483/1‑73 (MQ=255)                                                                   tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTc                                                              >  2:26721/1‑139 (MQ=255)                                                                   tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTc                                                              >  2:384414/1‑139 (MQ=255)                                                                          ttCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTg                                                       >  1:19009/1‑139 (MQ=255)                                                                                  gACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                                               <  1:1092590/139‑1 (MQ=255)                                                                                     tttGTTAACGTCATTTACAGAAATTGAC‑CATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                                        <  1:711312/62‑1 (MQ=255)                                                                                     tttGTTAACGTCATTTACAGAAATTGAC‑CATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                                        >  2:711312/1‑62 (MQ=255)                                                                                       tGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGc                                          <  2:19009/139‑1 (MQ=255)                                                                                           aaCGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAACGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAg                                      <  2:914362/139‑1 (MQ=255)                                                                                            aCGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcgc                                                                                                                             >  1:488421/1‑51 (MQ=255)                                                                                            aCGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcgc                                                                                                                             <  2:488421/51‑1 (MQ=255)                                                                                             cGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCt                                                     <  1:746920/122‑1 (MQ=255)                                                                                             cGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCt                                                     >  2:746920/1‑122 (MQ=255)                                                                                              gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCt                                                     >  1:104521/1‑121 (MQ=255)                                                                                              gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCt                                                     <  2:104521/121‑1 (MQ=255)                                                                                               tCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                  <  1:800967/139‑1 (MQ=255)                                                                                                   ttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATcc                              >  1:831753/1‑139 (MQ=255)                                                                                                              gACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcgcg                   >  1:758444/1‑139 (MQ=255)                                                                                                               acacATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCgcgc                  >  2:787438/1‑139 (MQ=255)                                                                                                                 acaTCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCa                >  2:1526443/1‑139 (MQ=255)                                                                                                                   aTCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                                                >  2:1472568/1‑73 (MQ=255)                                                                                                                   aTCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                                                <  1:1472568/73‑1 (MQ=255)                                                                                                                     cAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  2:1582939/76‑1 (MQ=255)                                                                                                                     cAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           >  1:1582939/1‑76 (MQ=255)                                                                                                                         tGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGt        >  1:116773/1‑139 (MQ=255)                                                                                                                             tGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           >  2:24241/1‑68 (MQ=255)                                                                                                                             tGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  1:24241/68‑1 (MQ=255)                                                                                                                             tGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCATGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcg    >  2:506167/1‑139 (MQ=255)                                                                                                                               cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcgcg  >  1:253001/1‑139 (MQ=255)                                                                                                                                  tACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGc                            <  2:1250663/110‑1 (MQ=255)                                                                                                                                  tACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGc                            >  1:1250663/1‑110 (MQ=255)                                                                                                                                      |                                                                                                                                    GAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCG  >  NC_000913/663969‑664233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑