Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 711,724 C→T A59T (GCG→ACG)  ybfE ← LexA‑regulated protein, CopB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913711,7240CT100.0% 154.1 / NA 48A59T (GCG→ACG) ybfELexA‑regulated protein, CopB family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/26);  total (22/26)

ACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCG  >  NC_000913/711589‑711857
                                                                                                                                       |                                                                                                                                     
aCATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGtt                                                                                                                                    <  2:712885/139‑1 (MQ=255)
    gAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGc                                                                                                                                >  2:408595/1‑139 (MQ=255)
    gAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGc                                                                                                                                >  2:432903/1‑139 (MQ=255)
      aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCtt                                                                                                                              >  1:1314672/1‑139 (MQ=255)
               tttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     <  2:1314672/139‑1 (MQ=255)
               tttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     <  1:153066/139‑1 (MQ=255)
                ttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGaca                                                                                                                    <  1:1321591/139‑1 (MQ=255)
                ttAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGaca                                                                                                                    <  2:1561137/139‑1 (MQ=255)
                      gATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTa                                                                                                              <  1:1404313/139‑1 (MQ=255)
                                     cAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTg                                                                                               >  2:1213497/1‑139 (MQ=255)
                                       gcgcCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTaa                                                                                                             >  2:1347127/1‑123 (MQ=255)
                                       gcgcCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTaa                                                                                                             <  1:1347127/123‑1 (MQ=255)
                                         gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     <  2:552312/113‑1 (MQ=255)
                                         gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     >  1:552312/1‑113 (MQ=255)
                                         gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgc                                                                                           >  1:567680/1‑139 (MQ=255)
                                             ccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGttt                                                                                       >  1:1499930/1‑139 (MQ=255)
                                                         ttAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           <  2:1385353/139‑1 (MQ=255)
                                                         ttAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           <  1:408595/139‑1 (MQ=255)
                                                         ttAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           <  1:321185/139‑1 (MQ=255)
                                                         ttAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           <  1:1213497/139‑1 (MQ=255)
                                                                   ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                              >  1:596259/1‑126 (MQ=255)
                                                                   ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGtt                                                                              <  2:596259/126‑1 (MQ=255)
                                                                       aTCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                         <  2:1147426/127‑1 (MQ=255)
                                                                       aTCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                         >  1:1147426/1‑127 (MQ=255)
                                                                                 tACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTa                                                                                                              <  1:1361125/80‑1 (MQ=255)
                                                                                 tACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTa                                                                                                              >  2:1361125/1‑80 (MQ=255)
                                                                                      cTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                               <  1:900322/122‑1 (MQ=255)
                                                                                      cTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                               >  2:900322/1‑122 (MQ=255)
                                                                                         atatTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCGGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGaaa                                           >  1:994444/1‑139 (MQ=255)
                                                                                          tatTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAg                                          <  2:567680/139‑1 (MQ=255)
                                                                                               cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTa                                                                                                              <  2:1162858/66‑1 (MQ=255)
                                                                                               cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTa                                                                                                              >  1:1162858/1‑66 (MQ=255)
                                                                                               cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                         <  2:415365/103‑1 (MQ=255)
                                                                                               cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGtt                                                                         >  1:415365/1‑103 (MQ=255)
                                                                                               cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACCCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                                     <  2:994444/139‑1 (MQ=255)
                                                                                                    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGtt                                                                                        >  2:598424/1‑83 (MQ=255)
                                                                                                    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGtt                                                                                        <  1:598424/83‑1 (MQ=255)
                                                                                                    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                               <  2:1121708/108‑1 (MQ=255)
                                                                                                    cTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                               >  1:1121708/1‑108 (MQ=255)
                                                                                                                 ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     <  1:616944/41‑1 (MQ=255)
                                                                                                                 ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                                     >  2:616944/1‑41 (MQ=255)
                                                                                                                 ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgcg                                                                                          <  2:13392/68‑1 (MQ=255)
                                                                                                                 ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgcg                                                                                          >  1:13392/1‑68 (MQ=255)
                                                                                                                 ttCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCg                   >  2:1300690/1‑139 (MQ=255)
                                                                                                                      cgcCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           >  2:601813/1‑78 (MQ=255)
                                                                                                                      cgcCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                           <  1:601813/78‑1 (MQ=255)
                                                                                                                                 cTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTgcgc   >  1:1113465/1‑139 (MQ=255)
                                                                                                                                  ttCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGcgcg  <  2:1188975/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCG  >  NC_000913/711589‑711857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: