Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,947	G→A	*226* (TAG→TAA)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,947	0	G	A	100.0%	132.0 / NA	41	*226* (TAG→TAA)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/18);  total (23/18)

CGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAT  >  NC_000913/812811‑813072                                                                                                                                         |                                                                                                                              cGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAAcc                                                                                                                             >  1:1406924/1‑139 (MQ=255)    gAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                          <  1:803879/139‑1 (MQ=255)             aCGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTg                                                                                                                 >  1:878867/1‑139 (MQ=255)                    atatatGA‑CACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAAtt                                                                                                         <  1:627304/139‑1 (MQ=255)                                gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTa                                                                                                                     <  2:406686/116‑1 (MQ=255)                                gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTa                                                                                                                     >  1:406686/1‑116 (MQ=255)                                gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                              <  2:1406924/139‑1 (MQ=255)                                 cTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                             <  2:878867/139‑1 (MQ=255)                                     cccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                         >  1:140896/1‑139 (MQ=255)                                      ccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTc                                                                                        >  1:800509/1‑139 (MQ=255)                                              ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcga                                                                                                     >  1:1447786/1‑118 (MQ=255)                                              ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcga                                                                                                     <  2:1447786/118‑1 (MQ=255)                                              ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTg                                                                                >  1:882706/1‑139 (MQ=255)                                                cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              >  2:673836/1‑139 (MQ=255)                                                 ccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                             <  1:1143286/139‑1 (MQ=255)                                                       cgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                         >  2:695811/1‑121 (MQ=255)                                                       cgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                         <  1:695811/121‑1 (MQ=255)                                                        gctgctGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAAttt                                                                      <  1:673836/139‑1 (MQ=255)                                                                      tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTa                                                                                                                     >  1:1126157/1‑78 (MQ=255)                                                                      tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTa                                                                                                                     <  2:1126157/78‑1 (MQ=255)                                                                           tGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgag                                                                                                    <  1:766209/90‑1 (MQ=255)                                                                           tGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgag                                                                                                    >  2:766209/1‑90 (MQ=255)                                                                           tGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaaa                                                           <  1:563853/131‑1 (MQ=255)                                                                           tGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaaa                                                           >  2:563853/1‑131 (MQ=255)                                                                             gCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              >  2:713865/1‑110 (MQ=255)                                                                             gCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              <  1:713865/110‑1 (MQ=255)                                                                               ttGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAaataat                                               >  2:1429699/1‑139 (MQ=255)                                                                                 gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                             >  2:584384/1‑91 (MQ=255)                                                                                 gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                             <  1:584384/91‑1 (MQ=255)                                                                                  cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCAc                                            >  1:1096301/1‑139 (MQ=255)                                                                                  cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAAATGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCAc                                            >  1:1158740/1‑139 (MQ=255)                                                                                     aaaaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaa                                         <  2:882706/139‑1 (MQ=255)                                                                                       aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                          <  2:1559825/56‑1 (MQ=255)                                                                                       aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                          >  1:1559825/1‑56 (MQ=255)                                                                                                   gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTc                           >  2:474693/1‑139 (MQ=255)                                                                                                          ccGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             >  2:670729/1‑98 (MQ=255)                                                                                                          ccGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             <  1:670729/98‑1 (MQ=255)                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                             <  2:1464474/77‑1 (MQ=255)                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                             >  1:1464474/1‑77 (MQ=255)                                                                                                                            ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt  >  1:740681/1‑139 (MQ=255)                                                                                                                            ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt  >  1:1094312/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                              CGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAT  >  NC_000913/812811‑813072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑