Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,242	C→T	P413P (CCG→CCA)	ybiO ←	mechanosensitive channel protein, intermediate conductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,242	0	C	T	100.0%	116.6 / NA	37	P413P (CCG→CCA)	ybiO	mechanosensitive channel protein, intermediate conductance
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

CGCCGTTTTGCAGCCAGTTCCAGAAATCGAACAATCCCCATGCGCTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCA  >  NC_000913/844106‑844356                                                                                                                                         |                                                                                                                   cgcCGTTTTGCAGCCAGTTCCAGAAATCGAACAATCCCCATGCGCTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTgg                                                                                                                  >  1:153700/1‑139 (MQ=255)                  ttCCAGAAATCGAACAATCCCCATGCGCTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTAt                                                                                                 >  1:1130175/1‑139 (MQ=255)                                             cTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCgcca                                                                      >  1:1026350/1‑139 (MQ=255)                                             cTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGATGAGTATGTGGCGAGAGAGTGATgg                                                                               <  2:1332512/130‑1 (MQ=255)                                             cTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGATGAGTATGTGGCGAGAGAGTGATgg                                                                               >  1:1332512/1‑130 (MQ=255)                                                aaCAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGTCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCgccagcc                                                                   <  1:1531034/139‑1 (MQ=255)                                                aaCAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCgccagcc                                                                   >  2:1149531/1‑139 (MQ=255)                                                  cAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGccagccag                                                                 <  1:1149531/139‑1 (MQ=255)                                                        cATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGaa                                                           <  2:1026350/139‑1 (MQ=255)                                                                  aCGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCga                                                                                          >  2:155221/1‑98 (MQ=255)                                                                  aCGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCga                                                                                          <  1:155221/98‑1 (MQ=255)                                                                   cGCAGACCGTCAGAATACGCGCCGGTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGa                                                >  2:985211/1‑139 (MQ=255)                                                                        aCTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGaaacaa                                           >  2:891085/1‑139 (MQ=255)                                                                         cTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGaaacaaa                                          <  1:985211/139‑1 (MQ=255)                                                                           gTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAGAACAt                                                      <  1:1339111/125‑1 (MQ=255)                                                                           gTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAGAACAt                                                      >  2:1339111/1‑125 (MQ=255)                                                                           gTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACg                                        <  1:445660/139‑1 (MQ=255)                                                                                aaTACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCg                                   <  2:292379/139‑1 (MQ=255)                                                                                aaTACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCg                                   <  2:153700/139‑1 (MQ=255)                                                                                  tACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGAt                                 >  2:1199065/1‑139 (MQ=255)                                                                                    cgcgCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATAc                               >  2:353564/1‑139 (MQ=255)                                                                                      cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACg                                                                                                         <  2:592714/63‑1 (MQ=255)                                                                                      cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACg                                                                                                         >  1:592714/1‑63 (MQ=255)                                                                                      cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTAtg                                                                                                >  1:246823/1‑72 (MQ=255)                                                                                      cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTAtg                                                                                                <  2:246823/72‑1 (MQ=255)                                                                                                   gccgccGACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGc                >  2:276916/1‑139 (MQ=255)                                                                                                       ccgACAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcg            <  1:891085/139‑1 (MQ=255)                                                                                                       ccgACAGCCAGCCATTCAACCGCGTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcg            <  1:1199065/139‑1 (MQ=255)                                                                                                          aCAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCgccagcc                                                                   >  2:115672/1‑81 (MQ=255)                                                                                                          aCAGCCAGCCATTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCgccagcc                                                                   <  1:115672/81‑1 (MQ=255)                                                                                                                 gccaTTCAACCGTTTTTGCAGCTCTGGATAGTTAc                                                                                                          <  2:609218/35‑1 (MQ=255)                                                                                                                 gccaTTCAACCGTTTTTGCAGCTCTGGATAGTTAc                                                                                                          >  1:609218/1‑35 (MQ=255)                                                                                                                 gccaTTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCa  <  2:120213/139‑1 (MQ=255)                                                                                                                  ccaTTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATAc                               <  2:423371/109‑1 (MQ=255)                                                                                                                  ccaTTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATAc                               >  1:423371/1‑109 (MQ=255)                                                                                                                   caTTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGc                                    <  1:717876/103‑1 (MQ=255)                                                                                                                   caTTCAACCGTTTTTGCAGCTCTGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGc                                    >  2:717876/1‑103 (MQ=255)                                                                                                                                      ctctGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGa                                                            >  2:26843/1‑60 (MQ=255)                                                                                                                                         |                                                                                                                   CGCCGTTTTGCAGCCAGTTCCAGAAATCGAACAATCCCCATGCGCTCAACAGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCA  >  NC_000913/844106‑844356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑