Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,229	C→T	*233* (TAG→TAA)	thiQ ←	thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,229	0	C	T	100.0%	217.4 / NA	67	*233* (TAG→TAA)	thiQ	thiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/32);  total (35/32)

CGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72095‑72361                                                                                                                                       |                                                                                                                                     cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAc                                                                                                                                  >  1:971743/1‑139 (MQ=255) cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAc                                                                                                                                  >  2:206347/1‑139 (MQ=255)    aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:971743/139‑1 (MQ=255)      aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  2:1117419/139‑1 (MQ=255)      aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             >  2:607489/1‑139 (MQ=255)         ggTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATc                                                                                                                          <  2:131937/139‑1 (MQ=255)           ttGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATccc                                                                                                                        >  2:581253/1‑139 (MQ=255)                  ttAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGGCGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGt                                                                                                                 <  1:1086107/139‑1 (MQ=255)                           aGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:1221066/116‑1 (MQ=255)                           aGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:1221066/1‑116 (MQ=255)                                 gtgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGcc                                                                                                  >  2:1415892/1‑139 (MQ=255)                                 gtgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGcc                                                                                                  >  2:680648/1‑139 (MQ=255)                                  tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  2:1178698/1‑139 (MQ=255)                                      ggTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTACATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 <  1:1398991/135‑1 (MQ=255)                                      ggTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTACATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  2:1398991/1‑135 (MQ=255)                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:670880/104‑1 (MQ=255)                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:670880/1‑104 (MQ=255)                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  1:1228531/106‑1 (MQ=255)                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             >  2:1228531/1‑106 (MQ=255)                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                            >  2:388918/1‑139 (MQ=255)                                              gATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaaca                                                                                     <  1:443693/139‑1 (MQ=255)                                                   gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:581253/139‑1 (MQ=255)                                                   gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:680648/139‑1 (MQ=255)                                                    aCGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAt                                                                               >  2:1353425/1‑139 (MQ=255)                                                     cGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              >  2:1334846/1‑139 (MQ=255)                                                     cGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              >  1:1363364/1‑139 (MQ=255)                                                            cgcgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATa                                                                       <  1:1178698/139‑1 (MQ=255)                                                             gcgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATAc                                                                      <  1:122306/139‑1 (MQ=255)                                                                tCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:1357276/1‑79 (MQ=255)                                                                tCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:1357276/79‑1 (MQ=255)                                                                           cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 <  2:1257942/98‑1 (MQ=255)                                                                           cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  1:1257942/1‑98 (MQ=255)                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  2:491910/1‑107 (MQ=255)                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:491910/107‑1 (MQ=255)                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTc                                                >  1:956466/1‑139 (MQ=255)                                                                                    gCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCg                                               <  2:1483672/139‑1 (MQ=255)                                                                                     cACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:1536675/105‑1 (MQ=255)                                                                                     cACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  2:1536675/1‑105 (MQ=255)                                                                                      aCTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             <  1:1415892/139‑1 (MQ=255)                                                                                               aGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                  <  1:294498/125‑1 (MQ=255)                                                                                               aGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                  >  2:294498/1‑125 (MQ=255)                                                                                                  tCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      <  1:1085258/70‑1 (MQ=255)                                                                                                  tCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      >  2:1085258/1‑70 (MQ=255)                                                                                                  tCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGa                                 >  2:616578/1‑139 (MQ=255)                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  2:672155/45‑1 (MQ=255)                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             >  1:672155/1‑45 (MQ=255)                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  2:237077/1‑139 (MQ=255)                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  2:1495359/1‑139 (MQ=255)                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCg                                               <  1:1588064/122‑1 (MQ=255)                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCg                                               >  2:1588064/1‑122 (MQ=255)                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgcg                              >  2:1099629/1‑139 (MQ=255)                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgcg                              >  1:1561870/1‑139 (MQ=255)                                                                                                       tAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      >  1:1043277/1‑65 (MQ=255)                                                                                                       tAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      <  2:1043277/65‑1 (MQ=255)                                                                                                          ggCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                            <  2:1209976/88‑1 (MQ=255)                                                                                                          ggCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                            >  1:1209976/1‑88 (MQ=255)                                                                                                                cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                             <  1:1565458/97‑1 (MQ=255)                                                                                                                cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                             >  2:1565458/1‑97 (MQ=255)                                                                                                                cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATcc                   >  2:677071/1‑139 (MQ=255)                                                                                                                       cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  1:607489/139‑1 (MQ=255)                                                                                                                       cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  1:1353425/139‑1 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      <  1:689689/39‑1 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACt                                                                                                      >  2:689689/1‑39 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            >  1:828726/1‑129 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  2:828726/129‑1 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  1:206347/139‑1 (MQ=255)                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGACGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:1363364/139‑1 (MQ=255)                                                                                                                                    atatTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 <  1:1525736/41‑1 (MQ=255)                                                                                                                                    atatTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  2:1525736/1‑41 (MQ=255)                                                                                                                                       |                                                                                                                                     CGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72095‑72361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑