Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,869	G→A	*301* (TAG→TAA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,869	0	G	A	100.0%	228.4 / NA	71	*301* (TAG→TAA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/37);  total (34/37)

TTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATGGCTC  >  NC_000913/892735‑893004                                                                                                                                       |                                                                                                                                        ttGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                     <  1:534748/139‑1 (MQ=255)   ggTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   <  2:1400218/139‑1 (MQ=255)   ggTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   <  1:419185/139‑1 (MQ=255)   ggTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   <  2:317285/139‑1 (MQ=255)          gatgtgtATGCGTCACCAGGGATGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACAtt                                                                                                                            <  1:461654/136‑1 (MQ=255)              tGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                <  1:962108/131‑1 (MQ=255)              tGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTg                                                                                                                        >  2:1529514/1‑139 (MQ=255)              tGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACAACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                >  2:962108/1‑131 (MQ=255)                aaTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATGGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   >  1:1138392/1‑126 (MQ=255)                aaTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   <  2:1138392/126‑1 (MQ=255)                 aTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                     <  2:113594/139‑1 (MQ=255)                 aTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                     <  1:155604/139‑1 (MQ=255)                  tGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACTGGTATCGACATCGCGGGTAAGATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATcaca                                                                                                                              >  1:1600035/1‑129 (MQ=255)                  tGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACTGGTATCGACATCGCGGGTAAGATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATcaca                                                                                                                              <  2:1600035/129‑1 (MQ=255)                            gggCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGcc                                                                                                          >  1:257736/1‑139 (MQ=255)                                 ggaaggaaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTgcgc                                                                                                     >  1:968743/1‑139 (MQ=255)                                       aaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                <  1:962143/106‑1 (MQ=255)                                       aaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                >  2:962143/1‑106 (MQ=255)                                       aaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTg                                                                                               >  2:165043/1‑139 (MQ=255)                                                 accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtgt                                                                                     >  2:75012/1‑139 (MQ=255)                                                     ccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCg                                                                                 <  2:968743/139‑1 (MQ=255)                                                      cGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCgg                                                                                >  2:1157815/1‑139 (MQ=255)                                                                 tCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAGACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCg                                                                     >  2:1305376/1‑139 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGt                                                                                                                <  2:863395/95‑1 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGt                                                                                                                >  1:863395/1‑95 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCtt                                                                            <  1:1398330/131‑1 (MQ=255)                                                                  cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCtt                                                                            >  2:1398330/1‑131 (MQ=255)                                                                   gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                     >  2:522636/1‑73 (MQ=255)                                                                   gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                     <  1:522636/73‑1 (MQ=255)                                                                     gggTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAag                                                                 <  1:165043/139‑1 (MQ=255)                                                                       gTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                <  1:1014837/74‑1 (MQ=255)                                                                       gTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                >  2:1014837/1‑74 (MQ=255)                                                                        tAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                              <  2:267382/139‑1 (MQ=255)                                                                              gATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCTATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                        <  1:1157815/139‑1 (MQ=255)                                                                              gATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                        <  1:924335/139‑1 (MQ=255)                                                                               aTCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACa                                                       <  2:257736/139‑1 (MQ=255)                                                                                    cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                        <  2:996525/85‑1 (MQ=255)                                                                                    cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                        >  1:996525/1‑85 (MQ=255)                                                                                    cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAAtt                                                  >  2:92195/1‑139 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                     <  1:1469827/48‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                     >  2:1469827/1‑48 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                <  1:489549/53‑1 (MQ=255)                                                                                            aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                                >  2:489549/1‑53 (MQ=255)                                                                                              cGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                              <  1:875732/85‑1 (MQ=255)                                                                                              cGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGc                                                                                              >  2:875732/1‑85 (MQ=255)                                                                                                  aCGCTACGACAGAATATTGCCTGAAGACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                                    <  1:1305376/139‑1 (MQ=255)                                                                                                  aCGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                                    >  2:428659/1‑139 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   <  2:1540960/43‑1 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                                   >  1:1540960/1‑43 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                                  <  1:545067/44‑1 (MQ=255)                                                                                                   cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                                  >  2:545067/1‑44 (MQ=255)                                                                                                        cGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                           >  1:1200238/1‑46 (MQ=255)                                                                                                        cGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                           <  2:1200238/46‑1 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGcc                                                                                                          <  1:1404667/57‑1 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGcc                                                                                                          >  2:1404667/1‑57 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                      >  1:483663/1‑109 (MQ=255)                                                                                                              aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                      <  2:483663/109‑1 (MQ=255)                                                                                                                tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCg                                         >  1:1130003/1‑120 (MQ=255)                                                                                                                tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCg                                         <  2:1130003/120‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                        >  1:193525/1‑84 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                        <  2:193525/84‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGt                                                          >  1:1430636/1‑98 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGt                                                          <  2:1430636/98‑1 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACg                 >  1:1275615/1‑139 (MQ=255)                                                                                                                     ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATAATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACg                 >  2:886036/1‑139 (MQ=255)                                                                                                                        gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                         >  2:450560/1‑96 (MQ=255)                                                                                                                        gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                         <  1:450560/96‑1 (MQ=255)                                                                                                                        gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTg              >  1:1426831/1‑139 (MQ=255)                                                                                                                               ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAt       >  2:869394/1‑139 (MQ=255)                                                                                                                               ggtggtTAATCGCAATCACATTACTGATAATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAt       <  1:886036/139‑1 (MQ=255)                                                                                                                                    ttAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATGGCTc  <  1:428659/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAATGGCTC  >  NC_000913/892735‑893004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑