Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,205	A→G	R76R (CGT→CGC)	aat ←	leucyl/phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,205	0	A	G	100.0%	230.3 / NA	66	R76R (CGT→CGC)	aat	leucyl/phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

CGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGAT  >  NC_000913/927081‑927338                                                                                                                             |                                                                                                                                      cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                                         <  2:1359122/139‑1 (MQ=255)   aCCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTg                                                                                                                       >  2:622974/1‑139 (MQ=255)      acgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                                    <  1:1160237/139‑1 (MQ=255)            cgcgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGcc                                                                                                              <  2:1149532/139‑1 (MQ=255)             gcgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCa                                                                                                             <  1:622974/139‑1 (MQ=255)                       ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                                   <  2:405676/139‑1 (MQ=255)                         ttCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcgcg                                                                                                 >  1:1170562/1‑139 (MQ=255)                             ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa                                                                                             >  2:582511/1‑139 (MQ=255)                             ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa                                                                                             >  2:1333930/1‑139 (MQ=255)                              tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGAt                                                                                            >  1:72545/1‑139 (MQ=255)                                  cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg                                                                                        >  2:569800/1‑139 (MQ=255)                                   gcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                                       <  1:68184/139‑1 (MQ=255)                                     gATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                                         >  1:772008/1‑103 (MQ=255)                                     gATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGGGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                                         <  2:772008/103‑1 (MQ=255)                                      aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                                    <  2:72545/139‑1 (MQ=255)                                      aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                                    <  2:343702/139‑1 (MQ=255)                                          cTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGaccacc                                                                                <  1:1333930/139‑1 (MQ=255)                                                   ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgg                                                                       >  1:621988/1‑139 (MQ=255)                                                   ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgg                                                                       >  1:1291124/1‑139 (MQ=255)                                                    cTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                                      <  1:1572897/139‑1 (MQ=255)                                                           gacctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg                                                                                      <  1:232359/116‑1 (MQ=255)                                                           gacctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg                                                                                      >  2:232359/1‑116 (MQ=255)                                                               tgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGgag                                                           >  2:66198/1‑139 (MQ=255)                                                                gaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGgaga                                                          >  2:116384/1‑139 (MQ=255)                                                                 accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTgg                                                                                                                <  2:1528484/84‑1 (MQ=255)                                                                 accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTgg                                                                                                                >  1:1528484/1‑84 (MQ=255)                                                                 accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                         <  2:1170562/139‑1 (MQ=255)                                                                 accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaa                                                         >  1:1329904/1‑139 (MQ=255)                                                                      aaGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCa                                                    <  2:621988/139‑1 (MQ=255)                                                                           tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGaa                                               <  1:569800/139‑1 (MQ=255)                                                                              ttCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcgcg                                                                                                 >  1:210583/1‑86 (MQ=255)                                                                              ttCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcgcg                                                                                                 <  2:210583/86‑1 (MQ=255)                                                                                        cacGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTg                                  >  2:69156/1‑139 (MQ=255)                                                                                         acGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgggg                                                                     >  2:449950/1‑103 (MQ=255)                                                                                         acGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgggg                                                                     <  1:449950/103‑1 (MQ=255)                                                                                         acGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTgg                                 >  1:1134870/1‑139 (MQ=255)                                                                                          cGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                                <  2:133465/139‑1 (MQ=255)                                                                                             tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGc                                                                 <  1:721277/103‑1 (MQ=255)                                                                                             tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGc                                                                 >  2:721277/1‑103 (MQ=255)                                                                                                ggCGAGCGTTTATGAAATCGCTTGATACTGCGGCTGATATGCAg                                                                                                                         <  2:1094058/44‑1 (MQ=38)                                                                                                ggCGAGCGTTTATGAAATCGCTTCATACTGCTACTGATATGCCGTGGTTCTGGCCATAGCCCCGCGCGGATATCCAGCTAACACCAGAGACTGGGGTCGACTGGTGAAAACCACGGAAAAATACCACGCTGGTaaacaa                          >  1:1048966/1‑135 (MQ=255)                                                                                                ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                                         >  1:1094058/1‑44 (MQ=255)                                                                                                ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt                                                                                                                  <  1:1247119/51‑1 (MQ=255)                                                                                                ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt                                                                                                                  >  2:1247119/1‑51 (MQ=255)                                                                                                  cGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                                    >  2:303100/1‑47 (MQ=255)                                                                                                  cGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                                    <  1:303100/47‑1 (MQ=255)                                                                                                    aGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCgggg                                                                                              <  2:1613492/67‑1 (MQ=255)                                                                                                    aGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCgggg                                                                                              >  1:1613492/1‑67 (MQ=255)                                                                                                     gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACg                                                  >  2:769448/1‑110 (MQ=255)                                                                                                     gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACg                                                  >  2:82023/1‑110 (MQ=255)                                                                                                     gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACg                                                  <  1:82023/110‑1 (MQ=255)                                                                                                     gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACg                                                  <  1:769448/110‑1 (MQ=255)                                                                                                     gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAAc                     >  1:1029761/1‑139 (MQ=255)                                                                                                      cGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATg                                                                        <  2:1512546/87‑1 (MQ=255)                                                                                                      cGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATg                                                                        >  1:1512546/1‑87 (MQ=255)                                                                                                       gTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                       <  2:482350/55‑1 (MQ=255)                                                                                                       gTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                       >  1:482350/1‑55 (MQ=255)                                                                                                            tGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg              <  2:1291124/139‑1 (MQ=255)                                                                                                            tGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg              <  1:66198/139‑1 (MQ=255)                                                                                                             gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             <  2:1134870/139‑1 (MQ=255)                                                                                                             gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             <  1:69156/139‑1 (MQ=255)                                                                                                                   gCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             >  2:1071685/1‑133 (MQ=255)                                                                                                                   gCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             <  1:1071685/133‑1 (MQ=255)                                                                                                                      tCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             >  2:1290455/1‑130 (MQ=255)                                                                                                                      tCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc             <  1:1290455/130‑1 (MQ=255)                                                                                                                        aTACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGAt  <  1:582511/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                      CGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGAT  >  NC_000913/927081‑927338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑