Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	942,735	A→G	M593V (ATG→GTG)	dmsA →	dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	942,735	0	A	G	100.0%	181.1 / NA	52	M593V (ATG→GTG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

CTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATC  >  NC_000913/942601‑942870                                                                                                                                       |                                                                                                                                        cTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCg                                                                                                                                     <  1:1349335/139‑1 (MQ=255)   tACGTGATTTTCAACGATCAGGTGATTAAACCCCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGc                                                                                                                                   <  2:1459424/139‑1 (MQ=255)                             tAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGACGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCg                                                                                                         >  2:408174/1‑139 (MQ=255)                                aCCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGAtt                                                                                                      >  1:1122426/1‑139 (MQ=255)                                  cgcgCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTcc                                                                                                    <  1:1599753/139‑1 (MQ=255)                                   gcgcTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCt                                                                                                   >  1:275502/1‑139 (MQ=255)                                   gcgcTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCt                                                                                                   <  1:408174/139‑1 (MQ=255)                                           aaTGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGcc                                                                                           <  2:443695/139‑1 (MQ=255)                                             tGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCaa                                                                                         <  1:141019/139‑1 (MQ=255)                                                 aGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGt                                                                                                                             >  2:626566/1‑99 (MQ=255)                                                 aGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGt                                                                                                                             <  1:626566/99‑1 (MQ=255)                                                     cATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAAc                                                                                        >  2:902555/1‑132 (MQ=255)                                                     cATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAAc                                                                                        <  1:902555/132‑1 (MQ=255)                                                         tATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGt                                                                             >  1:959510/1‑139 (MQ=255)                                                            gAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTcc                                                                          <  2:959510/139‑1 (MQ=255)                                                               aTGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCa                                                                       <  2:577392/139‑1 (MQ=255)                                                                 gACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGa                                                                                                        >  2:152212/1‑104 (MQ=255)                                                                 gACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGa                                                                                                        <  1:152212/104‑1 (MQ=255)                                                                  aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTg                                                                                   <  1:240767/124‑1 (MQ=255)                                                                  aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTg                                                                                   >  2:240767/1‑124 (MQ=255)                                                                  aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGc                                                                    >  1:699157/1‑139 (MQ=255)                                                                  aCCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGACGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGc                                                                    >  1:1016921/1‑139 (MQ=255)                                                                   ccAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCa                                                                   >  2:149297/1‑139 (MQ=255)                                                                   ccAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTACGCAAGCa                                                                   >  1:979878/1‑139 (MQ=255)                                                                     aGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCg                                                                         <  1:686642/131‑1 (MQ=255)                                                                     aGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCg                                                                         >  2:686642/1‑131 (MQ=255)                                                                       cGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAgggg                                                               >  2:352555/1‑139 (MQ=255)                                                                          aCTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATc                                                            <  2:1122426/139‑1 (MQ=255)                                                                            tGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCtt                                                          >  2:655566/1‑139 (MQ=255)                                                                              gCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTa                                                        >  2:673886/1‑139 (MQ=255)                                                                                     gTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcg                                                 <  2:275502/139‑1 (MQ=255)                                                                                       cTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGcgcg                                               >  2:212163/1‑139 (MQ=255)                                                                                                 aGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAgg                                     >  1:1143556/1‑139 (MQ=255)                                                                                                      cAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATc                                >  1:221976/1‑139 (MQ=255)                                                                                                         tttACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAggg                                                                >  1:853974/1‑104 (MQ=255)                                                                                                         tttACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAggg                                                                <  2:853974/104‑1 (MQ=255)                                                                                                           tACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAg                                                                     >  2:1508026/1‑97 (MQ=255)                                                                                                           tACTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAg                                                                     <  1:1508026/97‑1 (MQ=255)                                                                                                            aCTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTg                          <  2:699157/139‑1 (MQ=255)                                                                                                             cTGAAGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGc                         <  1:673886/139‑1 (MQ=255)                                                                                                                aaGGCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTta                      >  2:655835/1‑139 (MQ=255)                                                                                                                   gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGc                                                                                                          >  1:353277/1‑52 (MQ=255)                                                                                                                   gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGc                                                                                                          <  2:353277/52‑1 (MQ=255)                                                                                                                   gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcgc                                                >  2:1019826/1‑110 (MQ=255)                                                                                                                   gCCGTACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcgc                                                <  1:1019826/110‑1 (MQ=255)                                                                                                                       tACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGcc               >  1:1114230/1‑139 (MQ=255)                                                                                                                       tACCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCACAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGcc               >  2:574040/1‑139 (MQ=255)                                                                                                                        aCCCAGGAAGAGTGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCt              <  1:655835/139‑1 (MQ=255)                                                                                                                                    tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACg                             <  1:286749/112‑1 (MQ=255)                                                                                                                                    tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACg                             >  2:286749/1‑112 (MQ=255)                                                                                                                                    tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATc  >  2:442991/1‑139 (MQ=255)                                                                                                                                    tGGGTGCGGCATCTGTATGCCCAGTCGCGGGAAGCG‑TTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAgcg                                                 >  2:605167/1‑91 (MQ=255)                                                                                                                                       |                                                                                                                                        CTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGCTTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATC  >  NC_000913/942601‑942870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑