Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 965,917 A→G D533G (GAT→GGT)  ycaI → ComEC family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913965,9170AG100.0% 246.6 / NA 70D533G (GAT→GGT) ycaIComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/38);  total (32/38)

CGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGATAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATG  >  NC_000913/965785‑966049
                                                                                                                                    |                                                                                                                                    
cGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTgg                                                                                                                                >  2:749729/1‑139 (MQ=255)
     tgGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCGGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGcaac                                                                                                                           >  1:1466954/1‑139 (MQ=255)
       gCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGcaacaa                                                                                                                         <  2:1141830/139‑1 (MQ=255)
             aCCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGAtt                                                                                                                   <  1:749729/139‑1 (MQ=255)
                 cGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 >  1:58488/1‑121 (MQ=255)
                 cGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 <  2:779432/121‑1 (MQ=255)
                 cGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 <  2:58488/121‑1 (MQ=255)
                 cGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 >  1:779432/1‑121 (MQ=255)
                            gTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCAc                                                                                                    <  1:925373/139‑1 (MQ=255)
                            gTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCAc                                                                                                    >  2:611801/1‑139 (MQ=255)
                                      tACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGCCTGGCATGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACAGGCATAATc                                                                                          >  1:1106051/1‑139 (MQ=255)
                                          tGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGa                                                                                      >  2:1481621/1‑139 (MQ=255)
                                            cTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGAt                                                                                                                    <  1:505937/107‑1 (MQ=255)
                                            cTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGAt                                                                                                                    >  2:505937/1‑107 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCGGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                <  2:1466954/139‑1 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATcccc                                                                                                             >  2:33900/1‑110 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATcccc                                                                                                             <  1:33900/110‑1 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     >  1:398146/1‑118 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     <  2:398146/118‑1 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                >  1:1162779/1‑139 (MQ=255)
                                                aTGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCCCCACTGGCATAATCTTGAACCGGa                                                                                <  2:1100724/139‑1 (MQ=255)
                                                     gggCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCg                                                                           <  1:458738/139‑1 (MQ=255)
                                                     gggCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCg                                                                           >  2:475750/1‑139 (MQ=255)
                                                      ggCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                >  1:353323/1‑133 (MQ=255)
                                                      ggCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                <  2:353323/133‑1 (MQ=255)
                                                             gCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTg                                                                   <  2:518026/139‑1 (MQ=255)
                                                                      ggTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 <  2:1160845/68‑1 (MQ=255)
                                                                      ggTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTg                                                                                                                                 >  1:1160845/1‑68 (MQ=255)
                                                                       gTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAAc                                                         <  1:1045722/139‑1 (MQ=255)
                                                                       gTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAAc                                                         <  1:475750/139‑1 (MQ=255)
                                                                         gATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACAt                                                       <  2:695451/139‑1 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     <  2:1368812/91‑1 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     >  1:1368812/1‑91 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCg                                                                           >  1:202911/1‑117 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCg                                                                           <  2:202911/117‑1 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTAGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                <  1:705115/112‑1 (MQ=255)
                                                                           tAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTAGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGa                                                                                >  2:705115/1‑112 (MQ=255)
                                                                            aGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAg                                                                 <  1:1413767/126‑1 (MQ=255)
                                                                            aGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAg                                                                 >  2:1413767/1‑126 (MQ=255)
                                                                                 gAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCa                                               <  1:173266/139‑1 (MQ=255)
                                                                                  aaaCGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCAt                                                             <  2:679940/124‑1 (MQ=255)
                                                                                  aaaCGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCAt                                                             >  1:679940/1‑124 (MQ=255)
                                                                                  aaaCGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCAc                                              >  1:866443/1‑139 (MQ=255)
                                                                                             cGATTCTCTATGGCACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCt                                   <  1:64361/139‑1 (MQ=255)
                                                                                             cGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCt                                   <  2:1162779/139‑1 (MQ=255)
                                                                                                ttCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCt                                   <  2:1159186/136‑1 (MQ=255)
                                                                                                ttCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCt                                   >  1:1159186/1‑136 (MQ=255)
                                                                                                ttCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGa                                <  2:866443/139‑1 (MQ=255)
                                                                                                          cacaGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGAtt                              <  2:1297183/131‑1 (MQ=255)
                                                                                                          cacaGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGAtt                              >  1:1297183/1‑131 (MQ=255)
                                                                                                             aGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGc                                                                                                         <  1:1069228/53‑1 (MQ=255)
                                                                                                             aGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGc                                                                                                         >  2:1069228/1‑53 (MQ=255)
                                                                                                             aGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCat                   >  1:193894/1‑139 (MQ=255)
                                                                                                             aGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCAtt                                                            >  2:722831/1‑96 (MQ=255)
                                                                                                             aGGACTGGCCTGGCCTGAAGGGGGTAGTGGGCACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATg                                                            <  1:722831/97‑1 (MQ=255)
                                                                                                               gACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAAGTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACCTCTGGATCACCGGGGAGGGCTGGATTCAATATTGCatat                 >  1:146973/1‑139 (MQ=255)
                                                                                                               gACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACa                                                        <  1:677121/100‑1 (MQ=255)
                                                                                                               gACTGGCCTGGCCTGAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACa                                                        >  2:677121/1‑100 (MQ=255)
                                                                                                                    gcctggcctgAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCCTGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGc            <  2:1106051/139‑1 (MQ=255)
                                                                                                                    gcctggcctgAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGc            <  1:611801/139‑1 (MQ=255)
                                                                                                                      ctggcctgAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCg          <  1:1481621/139‑1 (MQ=255)
                                                                                                                         gcctgAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATg                                                            >  1:1405096/1‑86 (MQ=255)
                                                                                                                         gcctgAAGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATg                                                            <  2:1405096/86‑1 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     <  1:325019/40‑1 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCa                                                                                                     >  2:325019/1‑40 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCa                            >  1:350754/1‑113 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCa                            <  2:350754/113‑1 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATAtt                       >  1:1373204/1‑118 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATAtt                       <  2:1373204/118‑1 (MQ=255)
                                                                                                                              aaGGGGGTAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATg  >  1:1582530/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                    
CGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGGGCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGATAGTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATG  >  NC_000913/965785‑966049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: