Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,008	G→A	*453* (TAG→TAA)  V3I (GTT→ATT)	murF → mraY →	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,008	0	G	A	100.0%	194.6 / NA	60	*453* (TAG→TAA)  V3I (GTT→ATT)	murF mraY	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

TAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGC  >  NC_000913/95876‑96124                                                                                                                                     |                                                                                                                     tAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtggc                                                                                                                <  1:1106619/139‑1 (MQ=255)  aaaaCTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                 >  1:571247/1‑137 (MQ=255)  aaaaCTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                 <  2:571247/137‑1 (MQ=255)           ttAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACACGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACa                                                                                                      >  1:607680/1‑139 (MQ=255)             aaTTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                    <  1:814779/139‑1 (MQ=255)                    cgTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCaa                                                                                             >  1:658465/1‑139 (MQ=255)                           tataagagacagCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTTCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAAtattat                                                                                      <  2:1388667/128‑1 (MQ=255)                                 tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGc                                                                                >  2:676911/1‑139 (MQ=255)                                   aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                               >  1:1248591/1‑122 (MQ=255)                                   aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                               <  2:1248591/122‑1 (MQ=255)                                          aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAt                                                                                                     >  2:1426298/1‑109 (MQ=255)                                          aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAt                                                                                                     <  1:1426298/109‑1 (MQ=255)                                          aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTc                                                                                    >  2:1375280/1‑126 (MQ=255)                                          aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTc                                                                                    <  1:1375280/126‑1 (MQ=255)                                                 ggTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCt                                                                >  1:1446583/1‑139 (MQ=255)                                                  gTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCGATGGAAGAGGTAGTACGCGTTTAACAGGCGATTGGGAAAAGTCAATTGAGCTGGCCGAAGATTTGGTCAAATATTATTCCGGCTTTAActgtctcttata                                                               >  1:1388667/1‑128 (MQ=255)                                                    aaTTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATc                                                             >  2:1313806/1‑139 (MQ=255)                                                      ttACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                            >  2:556940/1‑122 (MQ=255)                                                      ttACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                            <  1:556940/122‑1 (MQ=255)                                                          gATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGt                                                       >  2:930953/1‑139 (MQ=255)                                                                         ttCACGTAGTGTCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                            <  1:138861/103‑1 (MQ=255)                                                                         ttCACGTAGTGTCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                            >  2:138861/1‑103 (MQ=255)                                                                           cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGTGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGcc                                               <  1:951624/130‑1 (MQ=255)                                                                           cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGcc                                               >  2:951624/1‑130 (MQ=255)                                                                                 gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                 <  2:944659/58‑1 (MQ=255)                                                                                 gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtgg                                                                                                                 >  1:944659/1‑58 (MQ=255)                                                                                 gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCctgctg                                >  2:1133062/1‑139 (MQ=255)                                                                                  tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                               >  2:348644/1‑139 (MQ=255)                                                                                     cgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                            <  2:1442071/139‑1 (MQ=255)                                                                                               gagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                  <  1:348644/139‑1 (MQ=255)                                                                                                 ggTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAtc                 >  2:102871/1‑138 (MQ=255)                                                                                                 ggTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAtc                 <  1:102871/138‑1 (MQ=255)                                                                                                      taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                <  2:688181/102‑1 (MQ=255)                                                                                                      taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                >  1:688181/1‑102 (MQ=255)                                                                                                      taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa              <  2:1081910/136‑1 (MQ=255)                                                                                                      taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa              >  1:1081910/1‑136 (MQ=255)                                                                                                      taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtg           >  2:45494/1‑139 (MQ=255)                                                                                                           gcTTTACAGGAGAATTGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt      >  1:1078341/1‑139 (MQ=255)                                                                                                           gcTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt      >  2:1027809/1‑139 (MQ=255)                                                                                                               tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  1:1519668/139‑1 (MQ=255)                                                                                                               tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  1:1133062/139‑1 (MQ=255)                                                                                                               tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  1:1027809/139‑1 (MQ=255)                                                                                                               tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  1:1313806/139‑1 (MQ=255)                                                                                                               tACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  2:658465/139‑1 (MQ=255)                                                                                                                    gagaATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCt                                                                <  2:1419260/72‑1 (MQ=255)                                                                                                                    gagaATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCt                                                                >  1:1419260/1‑72 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                               >  1:1253351/1‑36 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                               <  2:1253351/36‑1 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                  >  1:1031685/1‑113 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                  <  2:1031685/113‑1 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt      <  2:691600/125‑1 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt      >  1:691600/1‑125 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  >  1:1266556/1‑129 (MQ=255)                                                                                                                         tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  2:1266556/129‑1 (MQ=255)                                                                                                                              cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCtt                                                                     <  2:723388/57‑1 (MQ=255)                                                                                                                              cATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCtt                                                                     >  1:723388/1‑57 (MQ=255)                                                                                                                              cATGTTAATTTGGCTGGCCGAACATTTGGTC‑AATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  <  2:1413810/123‑1 (MQ=255)                                                                                                                              cATGTTAATTTGGCTGGCCGAACATTTGGTC‑AATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc  >  1:1413810/1‑123 (MQ=255)                                                                                                                                 gTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                          <  2:783280/97‑1 (MQ=255)                                                                                                                                 gTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                          >  1:783280/1‑97 (MQ=255)                                                                                                                                     |                                                                                                                     TAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGC  >  NC_000913/95876‑96124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑