Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	966,584	G→A	*755* (TAG→TAA)	ycaI →	ComEC family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	966,584	0	G	A	100.0%	229.3 / NA	70	*755* (TAG→TAA)	ycaI	ComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/34);  total (36/34)

ATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCG  >  NC_000913/966453‑966719                                                                                                                                    |                                                                                                                                        aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGc                                                                                                                                  >  1:292096/1‑139 (MQ=255)  ttGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTATGGTGTGCCAGTGGATAACGGGTAAAATATGCg                                                                                                                                 <  2:1077673/139‑1 (MQ=255)   tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                                <  2:292096/139‑1 (MQ=255)     aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCt                                                                                                                              >  1:1206058/1‑139 (MQ=255)           cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAat                                                                                                                                      <  1:687422/125‑1 (MQ=255)           cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAat                                                                                                                                      >  2:687422/1‑125 (MQ=255)           cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTca                                                                                                                        >  2:1340037/1‑139 (MQ=255)           cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTca                                                                                                                        >  2:1461544/1‑139 (MQ=255)             caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTcaac                                                                                                                      >  2:100657/1‑139 (MQ=255)             caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTcaac                                                                                                                      <  1:21028/139‑1 (MQ=255)             caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTcaac                                                                                                                      >  1:1113531/1‑139 (MQ=255)                cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACaaa                                                                                                                   >  2:668713/1‑139 (MQ=255)                  aGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                                 <  1:1461544/139‑1 (MQ=255)                   ggTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGc                                                                                                                >  1:378405/1‑139 (MQ=255)                   ggTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGAGTAGCAGCCTCAGGGAACAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGc                                                                                                                >  2:677969/1‑139 (MQ=255)                    gTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCt                                                                                                               <  2:1206058/139‑1 (MQ=255)                     tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTg                                                                                                              >  2:332339/1‑139 (MQ=255)                        aacaacGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGtt                                                                                                           >  2:459133/1‑139 (MQ=255)                            acGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGtttttt                                                                                                       <  1:668713/139‑1 (MQ=255)                               gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    <  1:196551/139‑1 (MQ=255)                               gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    >  2:1017031/1‑139 (MQ=255)                               gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    >  1:100942/1‑139 (MQ=255)                               gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    <  2:61259/139‑1 (MQ=255)                                    tttttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               <  1:459133/139‑1 (MQ=255)                                    tttttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               <  1:1352975/139‑1 (MQ=255)                                             gcAAGGCTGGCGGATTAACAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaa                                                                                       <  2:1236727/138‑1 (MQ=255)                                             gcAAGGCTGGCGGATTAACAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaa                                                                                       >  1:1236727/1‑138 (MQ=255)                                              cAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAg                                                                                     <  2:378405/139‑1 (MQ=255)                                               aaGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGa                                                                                    <  2:1113531/139‑1 (MQ=255)                                                aGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAt                                                                                   <  2:1536704/139‑1 (MQ=255)                                                 ggCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTAt                                                                                                                            <  2:549050/97‑1 (MQ=255)                                                 ggCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTAt                                                                                                                            >  1:549050/1‑97 (MQ=255)                                                                  ccTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACa                                                                 <  2:100942/139‑1 (MQ=255)                                                                   cTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAtct                                                                                 >  1:1581672/1‑122 (MQ=255)                                                                   cTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAtct                                                                                 <  2:1581672/122‑1 (MQ=255)                                                                            cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                                >  1:118768/1‑66 (MQ=255)                                                                            cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                                <  2:118768/66‑1 (MQ=255)                                                                            cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    >  1:145609/1‑94 (MQ=255)                                                                            cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                    <  2:145609/94‑1 (MQ=255)                                                                              aaTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAACATATGCGGCTATTTCAACAGATGCTGGTTTTTTGAATGCATAACGACAAAGAGCTCTCTCCGTGTCAGACATTCCGCCGACtg                                                     <  1:677969/139‑1 (MQ=255)                                                                              aaTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACtg                                                     <  1:100657/139‑1 (MQ=255)                                                                               aTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               <  2:908206/96‑1 (MQ=255)                                                                               aTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               >  1:908206/1‑96 (MQ=255)                                                                                               atcatcAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGcc                                    >  1:724268/1‑139 (MQ=255)                                                                                                   tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGc                                                                                                                               >  1:270191/1‑44 (MQ=255)                                                                                                   tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGc                                                                                                                               <  2:270191/44‑1 (MQ=255)                                                                                                     aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCt                                                                                                                              >  2:1348822/1‑43 (MQ=255)                                                                                                     aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCt                                                                                                                              <  1:1348822/43‑1 (MQ=255)                                                                                                     aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               <  2:1212680/74‑1 (MQ=255)                                                                                                     aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                               >  1:1212680/1‑74 (MQ=255)                                                                                                       tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                                 >  1:480909/1‑54 (MQ=255)                                                                                                       tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                                 <  2:480909/54‑1 (MQ=255)                                                                                                       tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                        <  1:223670/79‑1 (MQ=255)                                                                                                       tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                        >  2:223670/1‑79 (MQ=255)                                                                                                        ggTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                                 >  1:77918/1‑53 (MQ=255)                                                                                                        ggTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                                 <  2:77918/53‑1 (MQ=255)                                                                                                        ggTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                           <  2:724268/139‑1 (MQ=255)                                                                                                        ggTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                           >  1:962614/1‑139 (MQ=255)                                                                                                               cGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                            <  1:989611/131‑1 (MQ=255)                                                                                                               cGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                            >  2:989611/1‑131 (MQ=255)                                                                                                                   ccAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCg                >  1:165231/1‑139 (MQ=255)                                                                                                                            aaCGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACAt                                                                >  2:561933/1‑82 (MQ=255)                                                                                                                            aaCGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACAt                                                                <  1:561933/82‑1 (MQ=255)                                                                                                                              cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTa     >  2:11068/1‑139 (MQ=255)                                                                                                                               gggTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg    <  2:165231/139‑1 (MQ=255)                                                                                                                                ggTAAAATATGCGGCTATTTCAACAAATACTGGTTTTTTGAATGCATAACGAAAAAGATCTCTCTACGTGGCAGAAATTCAGCCGACTGTGGCCAACCATAGCGCCTTTCACAGCGGGTCTGATCGTGGCGGGCGTAGc   >  1:1560802/1‑139 (MQ=255)                                                                                                                                 gTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaaa                             >  2:1024664/1‑112 (MQ=255)                                                                                                                                 gTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                            >  1:1426428/1‑113 (MQ=255)                                                                                                                                 gTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                            <  2:1426428/113‑1 (MQ=255)                                                                                                                                 gTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCg  >  1:201172/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        ATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCG  >  NC_000913/966453‑966719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑