Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	983,623	G→A	*183* (TAG→TAA)	ycbK →	M15A protease‑related family periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	983,623	0	G	A	100.0%	216.8 / NA	66	*183* (TAG→TAA)	ycbK	M15A protease‑related family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/34);  total (32/34)

TCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAA  >  NC_000913/983496‑983758                                                                                                                                |                                                                                                                                        tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTaa                                                                                                                              <  1:1128618/139‑1 (MQ=255) tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTaa                                                                                                                              >  1:578487/1‑139 (MQ=255) tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTaa                                                                                                                              >  2:1397559/1‑139 (MQ=255) tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACAGCACTGGTAATAATTGCTTaa                                                                                                                              >  1:1083955/1‑139 (MQ=255)           aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACAGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                                    <  2:1083955/139‑1 (MQ=255)            ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   <  2:1342226/139‑1 (MQ=255)            ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   <  2:578487/139‑1 (MQ=255)               aTCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAg                                                                                                                >  2:1472699/1‑139 (MQ=255)                 cgcgTTAAGCAATATTCGCATAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACGGGGGCAgga                                                                                                              >  1:948689/1‑137 (MQ=255)                 cgcgTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                         >  2:544256/1‑128 (MQ=255)                 cgcgTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                         <  1:544256/128‑1 (MQ=255)                             tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       >  1:622217/1‑134 (MQ=255)                             tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       <  2:622217/134‑1 (MQ=255)                             tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGta                                                                                                  <  1:295296/139‑1 (MQ=255)                               ttCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTg                                                                                                                                   <  2:1218476/104‑1 (MQ=255)                               ttCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTg                                                                                                                                   >  1:1218476/1‑104 (MQ=255)                                                        cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   <  2:627392/95‑1 (MQ=255)                                                        cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   >  1:627392/1‑95 (MQ=255)                                                        cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                            <  1:133968/102‑1 (MQ=255)                                                        cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                            >  2:133968/1‑102 (MQ=255)                                                         gcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                      <  2:892627/139‑1 (MQ=255)                                                          cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                          <  2:582459/102‑1 (MQ=255)                                                          cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                          >  1:582459/1‑102 (MQ=255)                                                           aGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                                    <  1:1510634/139‑1 (MQ=255)                                                           aGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                                    <  2:1500895/139‑1 (MQ=255)                                                            ggtggtGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                   <  1:1397559/139‑1 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                          >  1:485477/1‑85 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                          <  2:485477/85‑1 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       >  2:1363048/1‑88 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       <  1:1363048/88‑1 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                   >  2:287030/1‑124 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                   <  1:287030/124‑1 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtg                                                    >  2:1453957/1‑139 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgt                                                     >  2:140880/1‑138 (MQ=255)                                                                           tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgt                                                     <  1:140880/138‑1 (MQ=255)                                                                                aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAAc                                               >  1:1438234/1‑139 (MQ=255)                                                                                   tagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACaaa                                            <  2:592040/139‑1 (MQ=255)                                                                                       aaCTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                                    <  2:141915/63‑1 (MQ=255)                                                                                       aaCTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                                    >  1:141915/1‑63 (MQ=255)                                                                                            tgtgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTgg                                   >  1:1404936/1‑139 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                         <  2:1236064/51‑1 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                         >  1:1236064/1‑51 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATtc                                                                              >  2:228504/1‑94 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATtc                                                                              <  1:228504/94‑1 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGtt                                                                 >  1:180594/1‑107 (MQ=255)                                                                                              tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGtt                                                                 <  2:180594/107‑1 (MQ=255)                                                                                                   atTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                   >  2:928367/1‑100 (MQ=255)                                                                                                   atTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                   <  1:928367/100‑1 (MQ=255)                                                                                                     tGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCa                                                                                                                 <  2:566424/52‑1 (MQ=255)                                                                                                     tGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCa                                                                                                                 >  1:566424/1‑52 (MQ=255)                                                                                                      gATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGt                         <  1:1453957/139‑1 (MQ=255)                                                                                                      gATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGt                         <  1:1472699/139‑1 (MQ=255)                                                                                                        tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTattatt                                                                                             >  2:18836/1‑69 (MQ=255)                                                                                                        tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTattatt                                                                                             <  1:18836/69‑1 (MQ=255)                                                                                                           cGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGa                                                                                                           <  1:1194380/52‑1 (MQ=255)                                                                                                           cGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGa                                                                                                           >  2:1194380/1‑52 (MQ=255)                                                                                                             ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   <  2:282785/42‑1 (MQ=255)                                                                                                             ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   >  1:282785/1‑42 (MQ=255)                                                                                                             ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                >  2:1336576/1‑77 (MQ=255)                                                                                                             ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                <  1:1336576/77‑1 (MQ=255)                                                                                                             ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt                  >  1:552385/1‑139 (MQ=255)                                                                                                              gCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   >  1:909519/1‑41 (MQ=255)                                                                                                              gCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                                   <  2:909519/41‑1 (MQ=255)                                                                                                                        cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       >  1:567924/1‑43 (MQ=255)                                                                                                                        cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                       <  2:567924/43‑1 (MQ=255)                                                                                                                        cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGc       >  2:1346212/1‑139 (MQ=255)                                                                                                                             gtaataATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGaaa  >  1:1428105/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                        TCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAA  >  NC_000913/983496‑983758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑