| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 988,952 | C→T | G12S (GGC→AGC) | asnS ← | asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 988,952 | 0 | C | T | 98.4% | 192.6 / NA | 62 | G12S (GGC→AGC) | asnS | asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (30/31); minor base G (1/0); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAG > NC_000913/988824‑989079 | gatgaCAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACg < 1:232089/139‑1 (MQ=255) gaCAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCg > 2:888965/1‑139 (MQ=255) ggATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa > 2:614356/1‑139 (MQ=255) gATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAAc > 2:311920/1‑139 (MQ=255) gATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAAc < 1:311920/139‑1 (MQ=255) tCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc < 1:888965/139‑1 (MQ=255) cAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTa > 2:1182681/1‑126 (MQ=255) cAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTa < 1:1182681/126‑1 (MQ=255) aaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCa > 1:1313908/1‑139 (MQ=255) aGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCataat < 2:1313908/139‑1 (MQ=255) gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTc < 2:38356/132‑1 (MQ=255) gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTc > 1:38356/1‑132 (MQ=255) gAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAatat > 2:657464/1‑139 (MQ=255) aaCCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATAtt > 2:898907/1‑139 (MQ=255) aCCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGTTACAGGCACAACGCTCATAATATtc < 2:791052/139‑1 (MQ=255) cGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctc < 1:898907/139‑1 (MQ=255) cGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctc < 1:614356/139‑1 (MQ=255) gTCATAAACGGCGAGGAAGGAGATGCCAGATTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACCCTCATAATATtctct > 2:1077796/1‑139 (MQ=255) tCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctctc > 1:298783/1‑139 (MQ=255) aTAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc > 2:1270096/1‑133 (MQ=255) aTAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc < 1:1270096/133‑1 (MQ=255) aCGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct < 2:1456044/132‑1 (MQ=255) aCGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct > 1:1456044/1‑132 (MQ=255) cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGt > 1:42238/1‑96 (MQ=255) cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGt < 2:42238/96‑1 (MQ=255) cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGt > 1:73807/1‑139 (MQ=255) cGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGGGACTTCGCTGTCAACGGCTACACGGCGCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCCGTTAATAGt > 1:744736/1‑139 (MQ=255) aaggaGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGaa < 2:15048/139‑1 (MQ=255) aggaGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGaaa < 2:73807/139‑1 (MQ=255) aTGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct > 2:1544506/1‑117 (MQ=255) aTGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct < 1:1544506/117‑1 (MQ=255) cAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAaca < 2:298783/139‑1 (MQ=255) cTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACtt > 2:391190/1‑139 (MQ=255) ttGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAATGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAATAAATAAACACTTGTc < 1:1077796/139‑1 (MQ=255) ttGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTc < 1:113231/139‑1 (MQ=255) gcggGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa > 2:474824/1‑139 (MQ=255) tacgtacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATgggg < 1:643677/139‑1 (MQ=255) acgtacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAGTAGTCGCTCTGTTAATAGTCGGAAAAAATAAACGCTTGGCCACCCGTAAATggggg < 2:744736/139‑1 (MQ=255) tacCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGAt > 2:1363719/1‑139 (MQ=255) aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa < 1:361649/66‑1 (MQ=255) aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACaa > 2:361649/1‑66 (MQ=255) aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATggggg < 1:371253/131‑1 (MQ=255) aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATggggg > 2:371253/1‑131 (MQ=255) aTCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTa > 1:395708/1‑139 (MQ=255) tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc > 2:124112/1‑79 (MQ=255) tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc < 1:124112/79‑1 (MQ=255) tCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTAt > 2:565655/1‑139 (MQ=255) ccGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc < 2:690165/67‑1 (MQ=255) ccGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGc > 1:690165/1‑67 (MQ=255) cgcACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa > 2:901172/1‑102 (MQ=255) cgcACGGTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa < 1:901172/102‑1 (MQ=255) ggTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa > 1:1391039/1‑97 (MQ=255) ggTGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATaaa < 2:1391039/97‑1 (MQ=255) tGACTTCGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCa > 2:1299943/1‑139 (MQ=255) cGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc > 1:1098950/1‑62 (MQ=255) cGCTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtc < 2:1098950/62‑1 (MQ=255) cTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa > 2:338091/1‑105 (MQ=255) cTGTCAACGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTaaa < 1:338091/105‑1 (MQ=255) aaCGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct > 1:905125/1‑58 (MQ=255) aaCGGCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATtctct < 2:905125/58‑1 (MQ=255) ggCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAg < 1:391190/139‑1 (MQ=255) ggCTACACGGCTCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAg < 1:1363719/139‑1 (MQ=255) | GATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTGCAATCAGACAAG > NC_000913/988824‑989079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |