Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	104,352	A→G	E124G (GAG→GGG)	ftsA →	ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	104,352	0	A	G	100.0%	309.0 / NA	85	E124G (GAG→GGG)	ftsA	ATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/43);  total (42/43)

CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATG  >  NC_000913/104222‑104482                                                                                                                                   |                                                                                                                                   cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                            <  1:926124/139‑1 (MQ=255) cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                              >  2:957368/1‑137 (MQ=255) cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                              <  1:957368/137‑1 (MQ=255)   ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  2:510124/139‑1 (MQ=255)   ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  2:545730/139‑1 (MQ=255)           cTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                  <  1:1072604/139‑1 (MQ=255)                  gCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           >  1:1522331/1‑139 (MQ=255)                  gCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           >  1:1303380/1‑139 (MQ=255)                   cacaTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGGAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTATGCTGCATGTGATCCCGc                                                                                                          <  1:896192/139‑1 (MQ=255)                      aTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                >  2:127388/1‑130 (MQ=255)                      aTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                <  1:127388/130‑1 (MQ=255)                      aTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAag                                                                                                       >  2:36964/1‑139 (MQ=255)                        cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAagag                                                                                                     >  1:1251912/1‑139 (MQ=255)                        cAGCTGCCAGAATGAAATTGGTATGGTACCTACTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAagag                                                                                                     >  1:959410/1‑139 (MQ=255)                                aGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCGTGTGATCCCGCAAGAGTATGCGAt                                                                                             >  2:902025/1‑139 (MQ=255)                                aGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAt                                                                                             <  2:236208/139‑1 (MQ=255)                                aGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAt                                                                                             >  2:1290864/1‑139 (MQ=255)                                 gAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt                                                                                            <  1:1290864/139‑1 (MQ=255)                                       aaTTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTAt                                                                                      <  2:1303380/139‑1 (MQ=255)                                       aaTTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTAt                                                                                      <  2:1522331/139‑1 (MQ=255)                                        aTTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                  >  2:896809/1‑94 (MQ=255)                                           ggTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  <  2:1251912/139‑1 (MQ=255)                                            gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  2:874403/98‑1 (MQ=255)                                            gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          >  1:874403/1‑98 (MQ=255)                                                  tGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                <  1:1209054/134‑1 (MQ=255)                                                  tGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                >  2:1209054/1‑134 (MQ=255)                                                  tGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGAt                                                                           >  1:478893/1‑139 (MQ=255)                                                      tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  2:1482619/88‑1 (MQ=255)                                                      tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          >  1:1482619/1‑88 (MQ=255)                                                       aTTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCATGAGTATGCGATTGACTATCAGGAAGGGATCAAGa                                                                      >  2:1034714/1‑139 (MQ=255)                                                        tttCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATGCCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACt                                                                                        <  2:1048672/120‑1 (MQ=255)                                                        tttCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCTTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACt                                                                                        >  1:1048672/1‑120 (MQ=255)                                                            tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCg                                                                 >  2:1035608/1‑139 (MQ=255)                                                                gaagaagTTACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAg                                                             <  2:959410/139‑1 (MQ=255)                                                                   gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATTGGTCCGTGTTCGCGATGGTCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAggcc                                                          >  2:287880/1‑137 (MQ=255)                                                                   gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAc                                                          >  2:352474/1‑139 (MQ=255)                                                                   gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAc                                                          >  2:929616/1‑139 (MQ=255)                                                                     agTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATAAGGAAGGGATCAAGAATCCGGTAGGACtt                                                        >  2:521283/1‑139 (MQ=255)                                                                         aCGCAAGACGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCgg                                                    >  2:718222/1‑139 (MQ=255)                                                                         aCGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCgg                                                    <  2:596185/139‑1 (MQ=255)                                                                              agaagaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTg                                               <  2:478893/139‑1 (MQ=255)                                                                              agaagaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTCCGGGCGTg                                               <  1:352474/139‑1 (MQ=255)                                                                                 agaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCgg                                            >  2:1373342/1‑139 (MQ=255)                                                                                    tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                  <  1:1169231/50‑1 (MQ=255)                                                                                    tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          >  2:875391/1‑58 (MQ=255)                                                                                    tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  1:875391/58‑1 (MQ=255)                                                                                    tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGaa                                                                     <  2:887477/111‑1 (MQ=255)                                                                                    tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGaa                                                                     >  1:887477/1‑111 (MQ=255)                                                                                     gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                  <  2:965116/49‑1 (MQ=255)                                                                                     gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                  >  1:965116/1‑49 (MQ=255)                                                                                       ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                 <  2:924063/48‑1 (MQ=255)                                                                                       ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                 >  1:924063/1‑48 (MQ=255)                                                                                       ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACt                                                                                        >  2:645258/1‑89 (MQ=255)                                                                                       ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACt                                                                                        <  1:645258/89‑1 (MQ=255)                                                                                             cgtcgtCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaaaa                                >  2:466336/1‑139 (MQ=255)                                                                                              gtcgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg                               <  1:1035608/139‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATc                                                                                                                               >  2:1223108/1‑41 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATc                                                                                                                               <  1:1223108/41‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct                                                                                                                        >  1:826320/1‑48 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct                                                                                                                        <  2:826320/48‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCATGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                             <  1:1034714/139‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  <  1:635003/86‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  >  2:635003/1‑86 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                             >  2:1018519/1‑139 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                             <  1:50021/139‑1 (MQ=255)                                                                                                cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                             <  1:245420/139‑1 (MQ=255)                                                                                                        ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                <  1:615841/80‑1 (MQ=255)                                                                                                        ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                >  2:615841/1‑80 (MQ=255)                                                                                                         cgcgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATc                    <  1:466336/139‑1 (MQ=255)                                                                                                           cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  >  1:1272767/1‑75 (MQ=255)                                                                                                           cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  <  2:1272767/75‑1 (MQ=255)                                                                                                           cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac                  >  1:1085174/1‑139 (MQ=255)                                                                                                             aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAAAGTATGCGATTGACTATCAGGATGGGATCAAGAATCCGGTAGGACTGTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACAt                <  1:287880/139‑1 (MQ=255)                                                                                                                        tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           >  2:662710/1‑37 (MQ=255)                                                                                                                        tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           <  1:662710/37‑1 (MQ=255)                                                                                                                        tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGat     >  2:1210096/1‑139 (MQ=255)                                                                                                                          tgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          >  1:1475650/1‑100 (MQ=255)                                                                                                                          tgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          <  2:1475650/100‑1 (MQ=255)                                                                                                                          tgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          >  2:1010387/1‑100 (MQ=255)                                                                                                                          tgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          <  1:1010387/100‑1 (MQ=255)                                                                                                                           gcgcGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATg  <  1:1018519/139‑1 (MQ=255)                                                                                                                            cgcgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATg                                         >  1:1311191/1‑99 (MQ=255)                                                                                                                            cgcgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATg                                         <  2:1311191/99‑1 (MQ=255)                                                                                                                              cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          >  1:1288386/1‑96 (MQ=255)                                                                                                                              cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                          <  2:1288386/96‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATG  >  NC_000913/104222‑104482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑