Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,117,958	A→G	G173G (GGT→GGC)	yceI ←	periplasmic high pH‑inducible lipid‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,117,958	0	A	G	100.0%	275.6 / NA	76	G173G (GGT→GGC)	yceI	periplasmic high pH‑inducible lipid‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/37);  total (39/37)

AACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCA  >  NC_000913/1117829‑1118093                                                                                                                                  |                                                                                                                                        aacaCTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCt                                                                                                                                >  2:644758/1‑139 (MQ=255)      tgtgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt                                                                                                                            >  2:1563151/1‑138 (MQ=255)      tgtgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt                                                                                                                            <  1:1563151/138‑1 (MQ=255)      tgtgCAGTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCtt                                                                                                                           >  1:1226645/1‑139 (MQ=255)        tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  >  2:895405/1‑130 (MQ=255)        tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  <  1:895405/130‑1 (MQ=255)        tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa                                                                                                                         >  2:1565934/1‑139 (MQ=255)        tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa                                                                                                                         >  1:258611/1‑139 (MQ=255)                    ccAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCt                                                                                                             >  2:513578/1‑139 (MQ=255)                       aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  <  2:1357267/115‑1 (MQ=255)                       aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  >  1:1357267/1‑115 (MQ=255)                         aGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGc                                                                                                        <  2:180696/139‑1 (MQ=255)                               aataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAAtt                                                                                                  <  2:258611/139‑1 (MQ=255)                                     aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  <  2:289009/101‑1 (MQ=255)                                     aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  >  1:289009/1‑101 (MQ=255)                                     aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt                                                                                                                            <  2:1463477/107‑1 (MQ=255)                                     aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt                                                                                                                            >  1:1463477/1‑107 (MQ=255)                                        gCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCg                                                                                         <  1:644758/139‑1 (MQ=255)                                         ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  <  2:791313/97‑1 (MQ=255)                                         ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  >  1:791313/1‑97 (MQ=255)                                         ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCt                                                                                                             <  1:1079741/118‑1 (MQ=255)                                         ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTGCTGCTATACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCAGTCTTGATATTGAAGTCCt                                                                                                             >  2:1079741/1‑118 (MQ=255)                                            gATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt                                                                                     <  1:1565934/139‑1 (MQ=255)                                             attcGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt                                                                                           <  2:1571488/129‑1 (MQ=255)                                             aTGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt                                                                                           >  1:1571488/1‑132 (MQ=255)                                                  tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGc                                                                               >  1:1389734/1‑139 (MQ=255)                                                       tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTaaa                                                                                                                                   >  2:1420967/1‑82 (MQ=255)                                                       tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTaaa                                                                                                                                   <  1:1420967/82‑1 (MQ=255)                                                        ccGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCAc                                                                         <  2:1226645/139‑1 (MQ=255)                                                            cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGa                                                                                                                   <  1:387659/93‑1 (MQ=255)                                                            cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGa                                                                                                                   >  2:387659/1‑93 (MQ=255)                                                               cATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacc                                                                  >  1:208986/1‑139 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  <  1:914060/53‑1 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt                                                                                                                                  >  2:914060/1‑53 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt                                                                                     >  2:159032/1‑98 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt                                                                                     <  1:159032/98‑1 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGa                                            >  1:12821/1‑139 (MQ=255)                                                                                     tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGa                                            <  2:12821/139‑1 (MQ=255)                                                                                       cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa                                                                                                                         <  2:701623/60‑1 (MQ=255)                                                                                       cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa                                                                                                                         >  1:701623/1‑60 (MQ=255)                                                                                       cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAcc                                          >  2:1051117/1‑139 (MQ=255)                                                                                       cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAcc                                          >  1:1486551/1‑139 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt                                                                                           >  2:1080524/1‑87 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt                                                                                           <  1:1080524/87‑1 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac                                                                   <  2:116958/111‑1 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac                                                                   >  1:116958/1‑111 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                >  2:1316905/1‑130 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                <  1:1316905/130‑1 (MQ=255)                                                                                          cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaat                                       >  2:870863/1‑139 (MQ=255)                                                                                            tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTg                                                                                                                    >  2:390148/1‑60 (MQ=255)                                                                                            tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTg                                                                                                                    <  1:390148/60‑1 (MQ=255)                                                                                            tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCt                                                                             >  2:1517840/1‑99 (MQ=255)                                                                                            tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCt                                                                             <  1:1517840/99‑1 (MQ=255)                                                                                                      taatCAGATCCACTTCCTGAGAAGCTGGGCCTAAATc                                                                                                                                 >  1:232399/1‑37 (MQ=255)                                                                                                      taatCAGATCCACTTCCTGAGAAGCTGGGCCTAAATc                                                                                                                                 <  2:232399/37‑1 (MQ=255)                                                                                                        atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTg                                                                                                                          <  1:299069/42‑1 (MQ=255)                                                                                                        atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTg                                                                                                                          >  2:299069/1‑42 (MQ=255)                                                                                                        atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc                                           <  1:389508/121‑1 (MQ=255)                                                                                                        atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc                                           >  2:389508/1‑121 (MQ=255)                                                                                                             aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac                                                                   >  1:1311258/1‑92 (MQ=255)                                                                                                             aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac                                                                   <  2:1311258/92‑1 (MQ=255)                                                                                                             aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTg                    <  1:1051117/139‑1 (MQ=255)                                                                                                              tCCACTTCCTGAGAAGCTGGGCTTAAATCTGTCTTGATATTGAAGTCCTTGGGCTTAATTTGGCCTTCGGCCTCGAAGCCGGCACGTTTACCACCCCTGGGGTCGTGGCCCTGACCATTTAATTTCGCTT‑CAGCGTGAc                  <  2:353942/139‑1 (MQ=255)                                                                                                              tCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                   <  2:328200/139‑1 (MQ=255)                                                                                                                 aCTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                   <  1:830912/136‑1 (MQ=255)                                                                                                                 aCTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                   >  2:830912/1‑136 (MQ=255)                                                                                                                  cTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg               >  1:602672/1‑139 (MQ=255)                                                                                                                   ttCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTT‑CAGCGTGACGGGtt             >  1:353942/1‑139 (MQ=255)                                                                                                                       tGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTgg          <  1:513578/139‑1 (MQ=255)                                                                                                                         agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt                                 <  2:470923/114‑1 (MQ=255)                                                                                                                         agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt                                 >  1:470923/1‑114 (MQ=255)                                                                                                                         agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa                         >  2:1014009/1‑122 (MQ=255)                                                                                                                         agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa                         <  1:1014009/122‑1 (MQ=255)                                                                                                                             gCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc    <  2:1596967/139‑1 (MQ=255)                                                                                                                             gCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc    >  1:1596967/1‑139 (MQ=255)                                                                                                                               tGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCa  <  2:208986/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        AACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCA  >  NC_000913/1117829‑1118093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑