Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,011	A→G	C106R (TGC→CGC)	mdtH ←	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,011	0	A	G	100.0%	167.0 / NA	47	C106R (TGC→CGC)	mdtH	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATAC  >  NC_000913/1124879‑1125139                                                                                                                                     |                                                                                                                                 tGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaaa                                                                                                                            >  1:588716/1‑139 (MQ=255)                tGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACgg                                                                                                             >  2:642805/1‑139 (MQ=255)                     tGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                        <  2:1376245/139‑1 (MQ=255)                            tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                >  1:1087049/1‑124 (MQ=255)                            tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                <  2:1087049/124‑1 (MQ=255)                                   cGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                <  2:373623/117‑1 (MQ=255)                                   cGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                >  1:373623/1‑117 (MQ=255)                                     agaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAAtgt                                                                                        >  2:1039838/1‑139 (MQ=255)                                                     ctgctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                        <  2:588716/139‑1 (MQ=255)                                                        ctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcgc                                                                     >  2:559043/1‑139 (MQ=255)                                                                          caccacCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                <  2:372662/78‑1 (MQ=255)                                                                          caccacCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa                                                                                                                >  1:372662/1‑78 (MQ=255)                                                                            ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaa                                                                                                                             <  1:1146081/63‑1 (MQ=255)                                                                            ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaa                                                                                                                             >  2:1146081/1‑63 (MQ=255)                                                                            ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGT‑GGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAatc                                                >  1:401886/1‑139 (MQ=255)                                                                                  gcgcCGAATGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCAt                                                             >  2:1049714/1‑121 (MQ=255)                                                                                  gcgcCGAACGCGGCGGATCAAACAAAGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAGCCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCAt                                                             <  1:1049714/121‑1 (MQ=255)                                                                                    gcCGAACGCGGCGGATCAAACAACGTGCCTCCGAGTCCCGAGAGCTGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCTGtt                                         <  1:1039838/139‑1 (MQ=255)                                                                                     ccGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcgg                                                                          >  2:1018831/1‑105 (MQ=255)                                                                                     ccGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcgg                                                                          <  1:1018831/105‑1 (MQ=255)                                                                                     ccGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGttt                                        >  1:677896/1‑139 (MQ=255)                                                                                      cGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTg                                       >  1:1015208/1‑139 (MQ=255)                                                                                             ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATtgtg                                                                                       >  2:474175/1‑84 (MQ=255)                                                                                             ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATtgtg                                                                                       <  1:474175/84‑1 (MQ=255)                                                                                                  aTCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCgg                           <  1:559043/139‑1 (MQ=255)                                                                                                           cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCg                                                                                                         <  2:1338356/52‑1 (MQ=255)                                                                                                           cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCg                                                                                                         >  1:1338356/1‑52 (MQ=255)                                                                                                           cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAAtt                  >  1:651439/1‑139 (MQ=255)                                                                                                           cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAAtt                  >  1:1301442/1‑139 (MQ=255)                                                                                                           cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCCCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAAtt                  >  2:1199853/1‑139 (MQ=255)                                                                                                            gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGtt                                         >  2:64048/1‑115 (MQ=255)                                                                                                            gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGtt                                         <  1:64048/115‑1 (MQ=255)                                                                                                              gCCACCGAGTCCCGAGAGAAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa               >  1:895390/1‑139 (MQ=255)                                                                                                               ccACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa               <  2:125351/138‑1 (MQ=255)                                                                                                               ccACCGAGTCCCGAGAACAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCa               >  1:125351/1‑138 (MQ=255)                                                                                                                cACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAcc             <  2:1015208/139‑1 (MQ=255)                                                                                                                  ccGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAccgc           <  1:681930/139‑1 (MQ=255)                                                                                                                     aGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGa        >  2:214869/1‑139 (MQ=255)                                                                                                                      gTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCCCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaa       <  1:1199853/139‑1 (MQ=255)                                                                                                                       tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                    <  2:393376/45‑1 (MQ=255)                                                                                                                       tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                    >  1:393376/1‑45 (MQ=255)                                                                                                                       tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaaa      <  1:214869/139‑1 (MQ=255)                                                                                                                           gagaGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATAc  <  2:274558/139‑1 (MQ=255)                                                                                                                                 aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcg                                                                           >  2:1586901/1‑60 (MQ=255)                                                                                                                                 aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcg                                                                           <  1:1586901/60‑1 (MQ=255)                                                                                                                                 aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAatcat                                              <  1:1364071/89‑1 (MQ=255)                                                                                                                                 aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAatcat                                              >  2:1364071/1‑89 (MQ=255)                                                                                                                                     |                                                                                                                                 TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATAC  >  NC_000913/1124879‑1125139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑