Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,109	G→A	*270* (TAG→TAA)	pabC →	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,109	0	G	A	100.0%	141.2 / NA	44	*270* (TAG→TAA)	pabC	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

GTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTACCC  >  NC_000913/1153977‑1154241                                                                                                                                     |                                                                                                                                     gTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGa                                                                                                                                >  2:1267264/1‑139 (MQ=255) gTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGa                                                                                                                                >  2:104122/1‑139 (MQ=255)       ggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAg                                                                                                                          >  2:224593/1‑139 (MQ=255)            atgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTa                                                                                                                     >  2:358942/1‑139 (MQ=255)                gatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGa                                                                                                                 <  1:1469848/139‑1 (MQ=255)                    gTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAAt                                                                                                             <  1:104122/139‑1 (MQ=255)                              aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                                   <  2:1273386/139‑1 (MQ=255)                               tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                           <  2:700297/114‑1 (MQ=255)                               tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                           >  1:700297/1‑114 (MQ=255)                               tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                                  >  2:965473/1‑139 (MQ=255)                               tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                                  >  2:103606/1‑139 (MQ=255)                                                    cGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccg                                                                             <  1:965473/139‑1 (MQ=255)                                                      tATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCt                                                                           >  2:648577/1‑139 (MQ=255)                                                       aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaa                                                                                                                            >  2:773407/1‑89 (MQ=255)                                                       aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaa                                                                                                                            <  1:773407/89‑1 (MQ=255)                                                               gtgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaa                                                                                                                             <  2:1332067/80‑1 (MQ=255)                                                               gtgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaa                                                                                                                             >  1:1332067/1‑80 (MQ=255)                                                                tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                               <  2:1490790/109‑1 (MQ=255)                                                                tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                               >  1:1490790/1‑109 (MQ=255)                                                                tgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg                                                                 >  2:1426797/1‑139 (MQ=255)                                                                     aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                       <  2:472021/80‑1 (MQ=255)                                                                     aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                       >  1:472021/1‑80 (MQ=255)                                                                      tGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccgc                                                                            <  1:600191/122‑1 (MQ=255)                                                                      tGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccgc                                                                            >  2:600191/1‑122 (MQ=255)                                                                             ttttCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCg                                                    <  1:1426797/139‑1 (MQ=255)                                                                                  gTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                   <  1:1561877/55‑1 (MQ=255)                                                                                  gTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCa                                                                                                                                   >  2:1561877/1‑55 (MQ=255)                                                                                         aCGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGa                                        <  1:224593/139‑1 (MQ=255)                                                                                          cGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGAc                                       >  2:952072/1‑139 (MQ=255)                                                                                                     tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                               >  1:1589719/1‑72 (MQ=255)                                                                                                     tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                               <  2:1589719/72‑1 (MQ=255)                                                                                                     tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCt                            >  1:295303/1‑139 (MQ=255)                                                                                                         tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGtt                                                                                                                      <  2:1276540/45‑1 (MQ=255)                                                                                                         tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGtt                                                                                                                      >  1:1276540/1‑45 (MQ=255)                                                                                                         tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTAtt                                                                                                    >  2:1105161/1‑63 (MQ=255)                                                                                                         tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTAtt                                                                                                    <  1:1105161/63‑1 (MQ=255)                                                                                                         tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATc                        >  2:840423/1‑139 (MQ=255)                                                                                                                aCTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGa                                                           >  1:816754/1‑97 (MQ=255)                                                                                                                aCTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGa                                                           <  2:816754/97‑1 (MQ=255)                                                                                                                 cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAagaaga                >  1:1412511/1‑139 (MQ=255)                                                                                                                   ttGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAgaga              <  2:843719/139‑1 (MQ=255)                                                                                                                               gAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTAccc  <  1:840423/139‑1 (MQ=255)                                                                                                                                aaTTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCg                                         <  2:352838/99‑1 (MQ=255)                                                                                                                                aaTTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCg                                         >  1:352838/1‑99 (MQ=255)                                                                                                                                     |                                                                                                                                     GTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTACCC  >  NC_000913/1153977‑1154241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑