Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,455	G→A	T132I (ACC→ATC)	chaA ←	calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,455	0	G	A	100.0%	118.8 / NA	37	T132I (ACC→ATC)	chaA	calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

TTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAA  >  NC_000913/1271321‑1271583                                                                                                                                       |                                                                                                                                 ttAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                                              <  1:1464587/139‑1 (MQ=255)                       aCCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                                              >  1:1055681/1‑117 (MQ=255)                       aCCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                                              <  2:1055681/117‑1 (MQ=255)                                     tCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                                          <  2:100304/139‑1 (MQ=255)                                                        tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCa                                                                                   >  2:413720/1‑127 (MQ=255)                                                        tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCa                                                                                   <  1:413720/127‑1 (MQ=255)                                                                 cAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGAtt                                                              >  1:368410/1‑139 (MQ=255)                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGcc                                                                                                               <  2:43069/87‑1 (MQ=255)                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGcc                                                                                                               >  1:43069/1‑87 (MQ=255)                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGcc                                                                                     <  2:1422249/113‑1 (MQ=255)                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGcc                                                                                     >  1:1422249/1‑113 (MQ=255)                                                                                   ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                                              >  1:1470789/1‑73 (MQ=255)                                                                                   ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                                              <  2:1470789/73‑1 (MQ=255)                                                                                   ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGc                                            >  1:35741/1‑139 (MQ=255)                                                                                             gcAATTAAATACTGCTTGATACCAAACAGATACATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTg                                  >  1:635761/1‑139 (MQ=255)                                                                                                         tGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                              >  1:230957/1‑83 (MQ=255)                                                                                                         tGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                              <  2:230957/83‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGAc                                                                                                                   >  2:539873/1‑44 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGAc                                                                                                                   <  1:539873/44‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                              >  1:566774/1‑81 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                                              <  2:566774/81‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATaa                                                                   >  2:74831/1‑92 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATaa                                                                   <  1:74831/92‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAAt                                                          >  2:1068323/1‑101 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAAt                                                          <  1:1068323/101‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATagag                                                      <  1:333399/105‑1 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATagag                                                      >  2:333399/1‑105 (MQ=255)                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCg                    >  1:1590047/1‑139 (MQ=255)                                                                                                                     aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGaa                                                                                                  <  2:1103288/51‑1 (MQ=255)                                                                                                                     aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGaa                                                                                                  >  1:1103288/1‑51 (MQ=255)                                                                                                                     aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                                     >  1:346216/1‑80 (MQ=255)                                                                                                                     aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                                     <  2:346216/80‑1 (MQ=255)                                                                                                                          aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                                           >  2:1447758/1‑85 (MQ=255)                                                                                                                          aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                                           <  1:1447758/85‑1 (MQ=255)                                                                                                                          aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCa                                           >  2:1396581/1‑101 (MQ=255)                                                                                                                          aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCa                                           <  1:1396581/101‑1 (MQ=255)                                                                                                                             cATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGaa  >  1:1578057/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 TTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAA  >  NC_000913/1271321‑1271583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑